ZHAO Xiaoming,ZHANG Ning,AN Xinyue.Role of miRNAs in Tomato Under Potassium Deficiency Stress[J].Northern Horticulture,2019,43(08):14-20.[doi:10.11937/bfyy.20183322]
MiRNAs在番茄钾营养胁迫中的作用
- Title:
- Role of miRNAs in Tomato Under Potassium Deficiency Stress
- Keywords:
- tomato; K+-deficiency stress; miRNAs; target gene
- 文献标志码:
- A
- 摘要:
- 以低钾不同耐性的2种基因型番茄（JZ18-钾敏感型和JZ34-耐低钾型）为试材，采用新一代高通量测序技术，对低钾胁迫下2种不同基因型番茄根系miRNAs进行测序，根据检测结果进行生物信息学分析，以期为研究响应低钾胁迫的关键miRNA提供参考依据。结果表明：低钾胁迫下，2个基因型番茄根系中表达差异显著，且表达趋势相反的miRNAs 包括miR167b、miR167g-5p、miR168a、miR171b。对其基因表达进行荧光实时定量(qRT-PCR)验证，并进一步对靶基因进行预测分析与功能研究，明确miR168a与其靶基因SlAGO1[STBZ]存在负调控关系并参与调控番茄的低钾胁迫响应。
- Abstract:
- High-throughput sequencing was performed using two tomato genotypes root (low-K+ sensitive JZ18 and low-K+ tolerant JZ34),and bioinformatics analysis was carried out according to the test results，in order to provide reference for studying key miRNAs in response to K+-deficiency stress.The results showed that the miRNAs (miR167b,miR167g-5p,miR168a,miR171b) were significantly expression and opposite expressed in the roots of the two genotypes under K+-deficiency stress.The gene expression was verified by real-time quantity (qRT-PCR),and the target gene was further predicted and functionally studied to confirm that miR168a had a negative regulatory relationship with its target gene SlAGO1,and was involved in the regulation of K+-deficiency stress response in tomato.
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备注/Memo
第一作者简介：赵晓明（1986-），女，博士，讲师，研究方向为蔬菜分子育种。E-mail:04012020zxm@163.com.基金项目：国家自然科学基金资助项目（31372054，31672138）；吉林农业科技学院青年基金资助项目（119012015027）。收稿日期：2018-12-17