LOU Xuan,TAO Gang,WANG Nian,et al.Colonization and Symbiosis Between Arbuscular Mycorrhizal Fungi and Pepper in Field[J].Northern Horticulture,2019,43(05):56-60.[doi:10.11937/bfyy.20182672]
丛枝菌根真菌与大田辣椒定殖共生研究
- Title:
- Colonization and Symbiosis Between Arbuscular Mycorrhizal Fungi and Pepper in Field
- Keywords:
- arbuscular mycorrhizal fungus; pepper; symbiosis; colonization; spore density
- 文献标志码:
- A
- 摘要:
- 以贵州省贵阳市3个辣椒种植地块的辣椒植株及根围土壤为试材，采用优化的湿筛倾注-蔗糖离心法从辣椒根围土壤中分离丛枝菌根真菌（AMF）孢子并测算根围中AMF孢子密度，乳酸甘油染色法对辣椒根样染色，观察辣椒根组织中AMF定殖情况，根据侵染分级强度法统计AMF的侵染率、侵染强度和丛枝丰度，对自然条件下辣椒根组织和根围中AMF的定殖共生进行了初步调查，以期为进一步研究AMF在辣椒大田种植生产中的作用提供依据和参考。结果表明：不同采样点的辣椒根组织中均发现AMF定殖及典型的共生丛枝菌根结构，侵染率达到70%以上；同时，在辣椒根际土壤中，存在丰富的AMF孢子，平均达到6个?g-1土壤。不同采样点辣椒的AMF侵染率、侵染强度和根际孢子量等存在差异，并随着AMF侵染强度的增强，丛枝丰度和孢子密度也随之增加。上述结果表明，在自然条件下，大田辣椒根际土壤中存在丰富的AMF，并且AMF能够在辣椒根组织中定殖共生。
- Abstract:
- The pepper plants and their rhizospheric soil samples from 3 pepper planting sites,Guiyang in Guizhou Province were collected as experimental materials.The AMF spores were extracted from soil samples by using optimized wet sieve decantation and sucrose centrifugation method，and the spore density in pepper rhizosphere was measured.The root samples of pepper were stained by the method of lactic acid glycerol staining，and the AMF colonization and their structures of arbuscular mycorrhizae (AM) in pepper roots were checked under the microscope.The AMF colonization rate，colonization intensity and arbuscular abundance were counted on the basis of the method of colonization grade intensity.The colonization and symbiosis between arbuscular mycorrhizal fungi (AMF) and pepper under natural conditions were primarily investigated.This study would provide the basis and reference for further research of the AMF roles for the cultivation and production of pepper.The results showed that AMF colonization and their AM structures within pepper roots were discovered among different sample sites，and the colonization rate was above 70%.Meanwhile，there were abundant AMF spores in rhizosphere of pepper at different sites，with an average of 6 spores?g-1 soil.The AMF colonization rate，colonization intensity and rhizospheric spore density were different among sample sites.With increasing the AMF colonization intensity，the AM abundance and rhizospheric spore density also increased.In this study，it was found that there were rich AMF in the pepper rhizosphere，and they could colonise in pepper roots and formed symbionts with pepper under the natural conditions.
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备注/Memo
第一作者简介：娄璇（1993-），女，硕士研究生，研究方向为植物真菌病害生物防治。E-mail：lxx621412@sina.com.责任作者：陶刚（1972-），男，博士，研究员，现主要从事植物真菌病害生物防治等研究工作。E-mail：ttg729@sina.com.基金项目：贵州省优秀青年科技人才专项资助项目（黔科合人字[2015]27号）；贵州省科学技术联合基金重点资助项目（黔科合J 字 LKN[2013]01 号）;国家自然科学基金资助项目（31860520）。收稿日期：2018-10-08