[1]张永强,刘贵群,黄建昌,等.十九份酸豆资源遗传多样性的SRAP标记分析[J].北方园艺,2018,42(23):32-36.[doi:10.11937/bfyy.20181522]
　ZHANG Yongqiang,LIU Guiqun,HUANG Jianchang,et al.Analysis of Genetic Diversity for Nineteen Genetic Resources of Tamarind (Tamarindus indica L.) by SRAP[J].Northern Horticulture,2018,42(23):32-36.[doi:10.11937/bfyy.20181522]
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十九份酸豆资源遗传多样性的SRAP标记分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年23 页码: 32-36 栏目: 出版日期: 2018-12-10
Title:
Analysis of Genetic Diversity for Nineteen Genetic Resources of Tamarind (Tamarindus indica L.) by SRAP
作者:
张永强1; 刘贵群2; 黄建昌2; 肖艳2
（1.广州市绿化公司，广东 广州 510440；2.仲恺农业工程学院 园艺园林学院，广东 广州 510225）
Author(s):
ZHANG Yongqiang1; LIU Guiqun2; HUANG Jianchang2; XIAO Yan2
(1.Guangzhou Landscaping Company,Guangzhou,Guangdong 510440；2.College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225)
关键词:
酸豆; 种质资源; SRAP; 遗传多样性
Keywords:
tamarind(Tamarindus indica L.); germplasm resources; SRAP; genetic diversity
DOI:
10.11937/bfyy.20181522
文献标志码:
A
摘要:
以19份酸豆资源为试材，采用SRAP标记技术，研究了酸豆遗传多样性，以期为酸豆资源的育种利用提供参考依据。结果表明:126对引物组合中有15对引物扩增条带清晰，多态性条带百分率大于25%，扩增出135条清晰的条带，其中72条为多态性条带，平均每对引物组合扩增出9条带，其中4.8条多态性条带，平均条带多态性百分率为53.12%，19份酸豆资源的遗传相似性系数在0.602~0.947，平均为0.791，UPGMA聚类分析，可将19份酸豆资源聚类四大类，野生材料之间的遗传差异小；非野生材料之间的遗传差异大于野生种。
Abstract:
In order to provide the foundation for the breeding,genetic diversity among 19 genetic resources of tamarind (Tamarindus indica L.) from Yunnan and Hainan was analyzed by using sequence-related amplified polymorphism (SRAP) markers.The results showed that 15 of 126 SRAP pairs of primers could amplify the clear and high polymorphism bands,polymorphic band percentage >25%.Using these 15 pairs of primers amplified 19 tamarinds by SRAP,a total of 135 amplified clear bands were obtained,of which 72 were polymorphic bands,per primer combination could amplify 9 bands,in which 4.8 polymorphic bands,the average percentage of polymorphic bands was 53.12%,the genetic similarity coefficient was 0.602-0.947,averaging 0.791;19 Tamarindus resources were divided into four classes by the results of UPGMA clustering.Genetic differences between wild materials were small,genetic differences between non-wild materials were greater than that of wild species.

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备注/Memo

第一作者简介：张永强(1969-)，男，本科，园林施工高级工程师和林业高级工程师，现主要从事风景园林施工和园林植物等研究工作。E-mail：zyq20020102@163.com.责任作者：黄建昌(1963-)，男，博士，教授，现主要从事园艺植物资源利用等研究工作。E-mail:jchuang63@126.com.收稿日期：2018-06-27

更新日期/Last Update: 2019-01-10