[1]孙浩元,杨丽,张俊环,等.砧木‘St.Julien A’组织培养快速繁殖技术[J].北方园艺,2018,42(21):80-84.[doi:10.11937/bfyy.20180786]
　SUN Haoyuan,YANG Li,ZHANG Junhuan,et al.Rapid Micropropagation of Rootstock ‘St.Julien A’[J].Northern Horticulture,2018,42(21):80-84.[doi:10.11937/bfyy.20180786]

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砧木‘St.Julien A’组织培养快速繁殖技术

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷期数: 2018年21 页码: 80-84 栏目: 出版日期: 2018-11-10

Title:: Rapid Micropropagation of Rootstock ‘St.Julien A’

作者:: 孙浩元; 杨丽; 张俊环; 姜凤超; 张宏; 王玉柱; （北京市林业果树科学研究院，国家林业局杏工程技术研究中心，北京落叶果树工程中心，北京林果业生态环境功能提升协同创新中心，北京 100093）

Author(s):: SUN Haoyuan; YANG Li; ZHANG Junhuan; JIANG Fengchao; ZHANG Hong; WANG Yuzhu; (Beijing Academy of Forestry and Pomology Sciences/Apricot Engineering and Technology Research Center of State Forestry Administration/Beijing Engineering Research Center for Deciduous Fruit Trees/Beijing Collaborative Innovation Center for Rco-environmental Improvement With Forestry and Fruit Trees,Beijing 100093)

关键词:: 李; 杏; 砧木; 组织培养; 快繁

Keywords:: plum; apricot; rootstock; tissue culture; rapid propagation

DOI:: 10.11937/bfyy.20180786

文献标志码:: A

摘要:: 以健康植株上的饱满芽和茎段为试材，采用组织培养方法，建立了李、杏砧木品种 ‘St.Julien A’无性繁殖技术体系,以期为李和杏规模化生产奠定基础。结果表明：以枝条上饱满的芽为外植体，以MS+BA 1.0 mg·L-1+IBA 0.1 mg·L-1作为初代培养基、WPM+BA 1.0 mg·L-1+IBA 0.1 mg·L-1作为增殖培养基、1/2MS+IBA 1.0 mg·L-1作为生根培养基，李、杏砧木品种‘St.Julien A’增殖系数可达8.5，生根率达96%；在蛭石基质中，移栽成活率达到91.6%。

Abstract:: The full buds and shoot segments of healthy plants were used for in vitro culture，a complete and practical micropropagation protocol for rapid vegetative propagation of the rootstock ‘St.Julien A’ was developed.The results showed that using fully developed buds as explants,MS+BA 1.0 mg·L-1+IBA 0.1 mg·L-1 as the initial medium,WPM+BA 1.0 mg·L-1+IBA 0.1 mg·L-1 as proliferation medium and 1/2MS+IBA 1.0 mg·L-1 as rooting medium,the proliferation coefficient and the rooting percentage reached 8.5 and 96%,respectively.Transplanted in the substrate of vermiculite,the survival percentage of transplanting plantlets reached 91.6%.

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备注/Memo

第一作者简介：孙浩元（1972-），男，博士，研究员，现主要从事杏资源育种等研究工作。E-mail：13691419570@139.com.责任作者：王玉柱（1960-），男，博士，研究员，现主要从事果树育种和栽培等研究工作。E-mail：chinabjwyz@126.com.基金项目：国家林业局948资助项目（2013-4-50）；科研创新平台建设资助项目（PT2018-09）。收稿日期：2018-04-16

更新日期/Last Update: 2018-12-19