[1]于沛侠,赵慧琪,刘勇,等.侵染番茄的黄瓜花叶病毒、烟草花叶病毒和马铃薯S病毒CP基因克隆及序列分析[J].北方园艺,2017,41(22):12-18.[doi:10.11937/bfyy.20171234]
　YU Peixia,ZHAO Huiqi,LIU Yong,et al.Cloning and Sequence Analysis of Coat Protein Genes of CMV,TMV and PVS From Lycopersicon esculentum Mill.Plant[J].Northern Horticulture,2017,41(22):12-18.[doi:10.11937/bfyy.20171234]
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侵染番茄的黄瓜花叶病毒、烟草花叶病毒和马铃薯S病毒CP基因克隆及序列分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年22 页码: 12-18 栏目: 出版日期: 2017-11-27
Title:
Cloning and Sequence Analysis of Coat Protein Genes of CMV,TMV and PVS From Lycopersicon esculentum Mill.Plant
作者:
于沛侠1; 赵慧琪1; 刘勇2; 王德富1; 牛颜冰1
1.山西农业大学 生命科学学院，山西 晋中 030801；2.湖南省农业科学院 植物保护研究所，湖南 长沙 410125
Author(s):
YU Peixia1; ZHAO Huiqi1; LIU Yong2; WANG Defu1; NIU Yanbing1
1.College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801；2.Hunan Plant Protection Institute,Hunan Academy of Agricultural Science,Changsha,Hunan 410125
关键词:
番茄; 复合侵染; 烟草花叶病毒; 黄瓜花叶病毒; 马铃薯S病毒
Keywords:
Lycopersicon esculentum Mill.; mixed infection; tobacco mosaic virus; cucumber mosaic virus; potato virus S
DOI:
10.11937/bfyy.20171234
文献标志码:
A
摘要:
以山西省晋中地区表现褪绿黄化、卷曲、叶脉变黑症状的番茄植株叶片为试材，采用双链RNA（double-stranded RNA,dsRNA）技术和非序列依赖PCR扩增（Sequence-independent amplification，SIA）方法，对侵染番茄病样的病毒病原进行检测，以期为山西省番茄病毒病的防治提供参考依据。结果表明：该病样由黄瓜花叶病毒（CMV）、烟草花叶病毒（TMV）和马铃薯S病毒（PVS）3种病毒复合侵染，将其分别命名为CMV-SXFQ、TMV-FQ和PVS-SXFQ。为明确CMV-SXFQ、TMV-FQ和PVS-SXFQ的分类地位，进一步克隆到了这3个病毒的CP基因序列并进行相似性比较，发现CMV-SXFQ与CMV各亚组代表株系相似性为74.9%~98.8%，氨基酸序列的相似性为82.5%~99.5%；TMV-FQ与TMV代表株系相似性为74.4%~99.8%，氨基酸序列的相似性为83.6%~100.0%；PVS-SXFQ与PVS代表株系相似性为93.2%~99.3%，氨基酸序列的相似性为92.4%~100.0%；系统进化分析表明：CMV-SXFQ分离物与亚组ⅠB的CH、HLJ、XJ2、Am、As株系亲缘关系最近；TMV-FQ分离物与IM、Jimo、SXFQ株系亲缘关系最近；PVS-SXFQ分离物与Cm、St株系亲缘关系最近。
Abstract:
The leaves of tomato (Lycopersicon esculentum) from Jinzhong region of Shanxi Province shown chlorotic yellows,curly,black leaf pathogen were used as materials,the technology of double-stranded RNA (dsRNA) and sequence-independent amplification(SIA) was used to identify the virus pathogen that induced tomato,in order to provide reference for the prevention and control of virus disease infecting tomato in Shanxi Province.The results showed that samples of plants were infected with tobacco mosaic virus(TMV),cucumber mosaic virus(CMV) and potato virus S(PVS) respectively and named CMV-SXFQ,TMV-FQ and PVS-SXFQ.To further characterize the isolate of CMV-SXFQ,TMV-FQ and PVS-SXFQ,the coat protain(CP) of CMV-SXFQ,TMV-FQ and PVS-SXFQ were cloned and analyzed.Sequence alignments showed that the nucleotide homology similarity between CMV-SXFQ and CMV subgroup virus were 74.9%-98.8%,and the amino acide sequence similarity were 82.5%-99.5%.The nucleotide homology similartity between TMV-FQ and TMV subgroup virus was 74.4%-99.5%,and the amino acide sequence similarity were 83.6%-100.0%.The nucleotide homology similartity between PVS-SXFQ and PVS subgroup virus were 93.3%-99.3%,and the amino acide sequence similartity were 92.4%-100.0%.Phylogenetic analysis suggested that CMV-SXFQ was the same as CH,HLJ,XJ2,Am and As,which belonged to the CMV subgroup I B;TMV-FQ was closely related to IM,Jimo and SXFQ;PVS-SXFQ was closely related to Cm,St.

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备注/Memo

第一作者简介：于沛侠（1992-），男，硕士研究生，研究方向为分子植物病毒学。E-mail：13546640863@163.com.

责任作者：牛颜冰（1968-），女，博士，教授，现主要从事分子植物病毒学和中药GAP等研究工作。E-mail：niuyanbingbest@163.com.
基金项目：公益性行业（农业）科研专项资助项目（201303028）；国家自然科学基金资助项目（31540050）。
收稿日期：2017-07-24

更新日期/Last Update: 2017-11-22