[1]刘丹丹,刘畅.甜瓜细菌性果斑病菌鉴定及杀菌剂毒力测定[J].北方园艺,2017,41(18):6-10.[doi:10.11937/bfyy.20170373]
　LIU Dandan,LIU Chang.Identification of Bacterial Fruit Blotch and Toxicity Test of Fungicides[J].Northern Horticulture,2017,41(18):6-10.[doi:10.11937/bfyy.20170373]
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甜瓜细菌性果斑病菌鉴定及杀菌剂毒力测定

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年18 页码: 6-10 栏目: 出版日期: 2017-09-30
Title:
Identification of Bacterial Fruit Blotch and Toxicity Test of Fungicides
作者:
刘丹丹; 刘畅
沈阳化工大学 环境与安全工程学院，辽宁 沈阳 110142
Author(s):
LIU Dandan; LIU Chang
Department of Environmental and Safety Engineering,Shenyang University of Chemical Technology,Shenyang,Liaoning 110142
关键词:
瓜类细菌性果斑病; 甜瓜; 杀菌剂; 毒力测定; 16S rRNA; 化学防治
Keywords:
Acidovorax citrulli; melon; fungicide; toxicity test; 16S rRNA; chemical prevention
DOI:
10.11937/bfyy.20170373
文献标志码:
A
摘要:
以内蒙古发病甜瓜上分离的病原细菌为试材，采用形态学、生理生化测定和16S rRNA序列比对的方法，进行了病原菌的生物学鉴定，并通过离体试验和活体试验相结合的方法，研究了不同杀菌剂对致病菌的防治效果，以期为细菌性果斑病的防治提供参考依据。结果表明:试验菌株为西瓜食酸菌(Acidovorax citrulli),毒力测定结果显示，不同杀菌剂对西瓜食酸菌的毒力差异较大，同一杀菌剂在离体和活体试验中的结果也不相同，需要2种试验方法相结合，才能对药剂生物活性作出准确评价。
Abstract:
The pathogenic bacteria were isolated and purified from the melon of Inner Mongolia.The morphological,physiological and biochemical tests and 16S rRNA sequence alignment were used to identify the pathogens.The results showed that the effects of different fungicides on pathogenic bacteria were studied by the combination of in vitro and in vivo experiments,and the virulence was evaluated comprehensively.Identification result showed that the strain was Acidovorax citrulli.The results showed that the virulence of different fungicides to pathogens varied greatly,and the effect of the same fungicides in vitro and in vivo was different.Two methods were needed to evaluate the bioactivity of the fungicides.

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备注/Memo

第一作者简介：刘丹丹（1981-），女，博士，讲师，现主要从事环境污染治理与修复及分子生物学与植物病害防治等研究工作。E-mail:liudandan.553@163.com.

基金项目：国家自然科学基金资助项目(31400481)；辽宁省博士科研启动资助项目(20141086)。
收稿日期：2017-05-15

更新日期/Last Update: 2017-09-30