[1]冯如,李泽宏,袁红慧,等.银杏两个WD40转录因子基因克隆及序列分析[J].北方园艺,2017,41(18):100-108.[doi:10.11937/bfyy.20170279]
　FENG Ru,LI Zehong,YUAN Honghui,et al.Cloning and Sequence Analysis of Two WD40 Transcription Factors in Ginkgo biloba L.[J].Northern Horticulture,2017,41(18):100-108.[doi:10.11937/bfyy.20170279]
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银杏两个WD40转录因子基因克隆及序列分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年18 页码: 100-108 栏目: 出版日期: 2017-09-30
Title:
Cloning and Sequence Analysis of Two WD40 Transcription Factors in Ginkgo biloba L.
作者:
冯如; 李泽宏; 袁红慧; 程华; 程水源
武汉轻工大学 生物与制药工程学院，湖北 武汉 430023
Author(s):
FENG Ru; LI Zehong; YUAN Honghui; CHENG Hua; CHENG Shuiyuan
School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan，Hubei 430023
关键词:
银杏; 类黄酮; WD40转录因子; 基因克隆
Keywords:
Ginkgo biloba L.; flavonoid; WD40 transcription factor; gene cloning
DOI:
10.11937/bfyy.20170279
文献标志码:
A
摘要:
WD40转录因子也称WDR（WD repeat）蛋白，是一种古老蛋白家族，参与调节类黄酮次生代谢途径中多种酶基因的表达，从而影响类黄酮的积累。以三年生银杏苗为试材，采用RT-PCR和RACE-PCR方法，研究了银杏WD40基因序列，以期深入了解银杏叶类黄酮合成代谢的分子机理。结果表明：GbWD401序列包含一段2 313 bp的开放阅读框，编码一个770 aa的肽链；GbWD402序列包含一段1 605 bp的开放阅读框，编码一个534 aa的肽链。银杏GbWD401和GbWD402基因推测编码蛋白序列均含有典型WD40重复基序。GbWD401蛋白序列与无油樟AtWD401相似性为49%，GbWD402蛋白序列与无油樟AtWD402相似性为78%。系统进化树发现，GbWD401与无油樟AtWD401、荷花NnWD40划归为同一分支；而GbWD402与无油樟AtWD402划归为另一分支。
Abstract:
WD40 transcription factor,also known as WDR (WD repeat),was an ancient family of proteins.It could participate in a few genes′ co-expression in the plant flavonoid secondary metabolic pathway,effectively improved the contents of flavonoids.This study used triennial gingko trees as materials,used RT-PCR and RACE-PCR technology explored the sequences of WD40,in order to understand the molecular mechanism and transcriptional regulation mechanisms of flavonoid metabolism in Ginkgo.The results showed that GbWD401 contained a 2 313 bp ORF encoding 770 amino acid residues,and GbWD402 contained a 1 605 bp ORF encoding 534 amino acid residues.The predicted protein sequences of GbWD401 and GbWD402 genes both contained the typical WD40 structural domain.GbWD401 shared 49% similarity with AtWD401 of Amborella trichopoda, GbWD402 shared 78% similarity with AtWD402 of Amborella trichopoda.Phylogenetic tree analysis showed that GbWD401 was grouped into the same clade with AtWD401 of Amborella trichopoda and NnWD40 of Nelumbo nucifera,while GbWD402 was grouped into the other clade with AtWD402 of Amborella trichopoda.

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备注/Memo

第一作者简介：冯如（1992-），男，硕士研究生，研究方向为生药学。E-mail：799809864@qq.com.

责任作者：程水源（1965-），男，博士，教授，研究方向为经济林种质资源评价与利用。E-mail：s_y_cheng@sina.com.
基金项目：国家自然科学基金资助项目（31270717,31400556）；湖北省自然科学重点资助项目（2013BHE029）。
收稿日期：2017-05-16

更新日期/Last Update: 2017-10-06