GENG Jing-zhang.Ginkgolic Acid Extraction from Seed Coat of Ginkgo biloba L.[J].Northern Horticulture,2012,36(13):42-44.
银杏种皮的银杏酸提取工艺优化研究
- Title:
- Ginkgolic Acid Extraction from Seed Coat of Ginkgo biloba L.
- 文章编号:
- 1001-0009(2012)13-0042-03
- 分类号:
- TS 255.1
- 文献标志码:
- A
- 摘要:
- 以银杏的种皮为试验材料，进行银杏酸提取工艺研究，并通过L9(34)正交实验探讨了料液比、提取溶剂浓度、时间、温度对提取效果的影响。结果表明：影响银杏酸提取效果的最主要因素是温度，并得出银杏酸提取的最佳工艺条件为：料液比1∶40、甲醇浓度90%、时间2.5 h、温度60℃，在此条件下分别在242、310 nm检测，含量分别为5.946、3.722 mg/g。
- Abstract:
- With seed coat of Ginkgo biloba as matierials，by the L9 (34) orthiogonal experiment，the Ginkgolic acid was extracted from Ginkgo biloba，the influences of the ratio of solid and liquid，extraction solvent concentration，time and temperature on the extraction effect were investigated.The results showed that the most important factor acid was temperature and the optimum reaction condition was：the ratio of solid and liquid 1∶40，90% methanol concentration，time 2.5 h，temperature 60℃.The best extraction content of Ginkgolic acid at 242 nm，310 nm were 5.946 mg/g and 3.722 mg/g.
参考文献/References:
[1]郝立勤.云南银杏产业的发展前景[J].资源开发与市场,1999,15(4):210-212.
[2]郑玉芝.银杏叶提取物及其在保健食品中的应用[J].食品工业科技,1996(2):47-48.
[3]李艳.银杏叶中槲皮素黄酮苷类抗紫外线作用的研究[J].中草药,1999,30(9):671-673．
[4]黄桂宽.银杏叶多糖的化学研究[J].中草药,1997,28(8):459-461．
[5]仰榴青,吴向阳,陈钧,等.银杏的分光光度法测定[J].分析化学,2004,32(5):661-664.
[6]潘苏华,董李娜.不容忽视的毒性物质-银杏酸[J].中国现代药物应用,2007(12):107-108.
[7]Baron-Ruppen G,Luepke N P.Evidence for toxic effects of alkyl phehols from Ginkgo biloha in the hen’s egg test(HET) [J]．Phytomedicine,2001,8(2):133-138.
[8]Hecker H,Johannisson R,Koch E,et al.In vitro evaluation of the cytotoxic potential of alkyl phenols from Ginkgo biloba L[J].Toxicology,2002,177(23):167-177.
[9]Itokawa I,Totsuka N,Nakahara K.Antitumor principles from Ginkgo biloba L.[J].Chem.Pharon.Bull,1987,35(7):3016.
[10]Lee J S,Cho Y S,Park E J,et al.Phospholipase Crl inhibitory principles from the sarcotestas of Ginkgo biloba[J].J Nat Prond,1998,61:867-871.
[11]赵成林,吕建峰.银杏外种皮中酚酸性成分的抗真菌作用[J].江苏药学与临床研究,2004,12(1):32-33.
[12]姜晓明,赵献军.银杏外种皮提取物对霉菌的抑制作用[J].西北农业学报,2007,16(6):30-33.
[13]倪学文,吴谋成.银杏外种皮中银杏酚酸的提取及应用研究[J].湖北中医学院学报,2001,3(4):22-24．
备注/Memo
作者简介：耿敬章(1980-)，男，硕士，讲师，研究方向为食品质量控制与资源开发利用。E-mail：jingz-geng@163.com.
基金项目：陕西理工学院科研资助项目(SLGKY11-05)。
收稿日期：2012-03-26