HAN Wenqing,WANG Jianguo,HE Yuanyuan,et al.Cultivation of Coprinus comatus Using the Fermented Chinese Medicine Residue From Yinqiao Detoxication Pills Production Waste and Analysis of the Active Pharmaceutical Ingredient[J].Northern Horticulture,2017,41(02):145-149.[doi:10.11937/bfyy.201702035]
银翘复方药渣发酵处理试栽毛头鬼伞及主要药物成分分析
- Title:
- Cultivation of Coprinus comatus Using the Fermented Chinese Medicine Residue From Yinqiao Detoxication Pills Production Waste and Analysis of the Active Pharmaceutical Ingredient
- Keywords:
- Yingqiao residue; stack fermentation; cultivation; Coprinus comatus; chlorogenic acid; arctiin
- 文献标志码:
- A
- 摘要:
- 为回收利用生产银翘片废弃药渣，以毛头鬼伞为供试菌株，发酵处理银翘药渣；建堆配料设计原则为控制总料C/N接近40∶1，以玉米芯为主料（配合添加奶牛粪、尿素），逐渐增大银翘药渣用量，以替代玉米芯；堆料共采用4个配方，配方A（CK）：玉米芯200 kg，牛粪100 kg，尿素3.5 kg，辅料6%（生石灰2%、石膏2%、过磷酸钙2%）；配方B：玉米芯100 kg，银翘药渣50 kg，牛粪50 kg，尿素1.0 kg，辅料6%；配方C：玉米芯75 kg，银翘药渣100 kg，牛粪50 kg，辅料6%；配方D：玉米芯50 kg，银翘药渣150 kg，牛粪50 kg，辅料6%。4个配方堆料总干质量均在200 kg以上，依墙建堆成半圆锥形（h≥1.0 m，r≥1.4 m）；发酵升温14 d（4-3-3-2-2）、经4次翻堆后，摊料降温装袋接种，待菌丝满袋后脱袋覆土出菇。结果表明：以配方C的产出率为最高，其相应生产指标为，初始C/N为41.66、终点C/N为23.71、腐熟度T为0.569、接种满袋率93.7%、菌袋平均产量381.9 g?kg-1、生物学效率41.0%。经HPLC检测，配方C发酵料中绿原酸、牛蒡苷含量分别为5.20～5.40、4.49～5.30 μg?g-1，其栽培所得子实体中绿原酸、牛蒡苷含量分别为0.40～0.45、3.81～4.27 μg?g-1，其中牛蒡苷从栽培料到子实体的迁移率达84.93%。
- Abstract:
- Coprinus comatus was cultivated by using the fermented Chinese medicine residue from Yinqiao detoxication pills production waste to recycle it.Corncobs were used as the main materials added with cow dung and urea to design the stack with C/N as 40∶1.Four designs A,B,C,D were adopted to build the stacks for controlling with Design A：corncobs 200 kg，cow dung 100 kg，urea 3.5 kg，accessories 6%(lime 2%,gypsum 2%,calcium superphosphate 2%).Design B：corncobs 100 kg，Yingqiao residue 50 kg，cow dung 50 kg，urea 1 kg，accessories 6%(lime 2%,gypsum 2%,calcium superphosphate 2%).Design C：corncobs 75 kg，Yingqiao residue 100 kg，cow dung 50 kg，accessories 6%(lime 2%,gypsum 2%,calcium superphosphate 2%) and Design D：corncobs 50 kg，Yingqiao residue 150 kg，cow dung 50 kg，accessories 6%(lime 2%,gypsum 2%，calcium superphosphate 2%).The dry cultivating substrate weighing over 200 kg were stacked against the wall and shaped as a semicone.After 14 days of fermentation and four times of turning-over of the stacks (respectively on the fourth day，the seventh day，the tenth day and the twelfth day)，the cultivating substrate were packed in the bags and innoculated.When the hyphae was full of the bag，it was taken out of the bag and cased with soil to make fruit developed.The results showed that the fruit body yield of Design C was the highest of the four designs，with the parametres of it as initial C/N 41.66，final C/N 23.71，T value 0.569，the bag filling ratio of innoculation 93.7%，the even bag yield of mushroom 381.9 g?kg-1，and the biological efficiency 41.0%.As the HPLC showed，the contents of chlorogenic acid and arctiin in the fermented cultivating substrate of Design C were 5.20-5.40 μg?g-1,4.49-5.30 μg?g-1 respectively and they were 0.40-0.45 μg?g-1,3.81-4.27 μg?g-1 in the fruit body.The transferring rate of arctiin from the cultivating substrate to the fruit body was high up to more than 84.93%.
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备注/Memo
第一作者简介：韩文清(1971-)，男，硕士，副教授，现主要从事微生物应用等研究工作。E-mail：hanwenqing7@126.com.基金项目：内蒙古自治区高等学校科学技术研究资助项目（NJZC14359）。