LU Xiaoxiao,GUO Weitao,ZHOU Junguo,et al.Induction of Callus and Regeneration of Cucurbita maxima Duch.in vitro[J].Northern Horticulture,2017,41(15):42-47.[doi:10.11937/bfyy.20165033]
印度南瓜愈伤组织诱导及其离体再生培养
- Title:
- Induction of Callus and Regeneration of Cucurbita maxima Duch.in vitro
- Keywords:
- Cucurbita maxima; explants; callus; regeneration
- 文献标志码:
- A
- 摘要:
- 以印度南瓜“北观”幼苗的下胚轴、子叶、真叶、茎段为外植体，采用离体再生方法，研究了不同种类激素对愈伤组织发育及离体再生的影响，以期为建立高效的南瓜再生体系奠定基础。结果表明：以印度南瓜幼苗的下胚轴、子叶、真叶、茎段为外植体均可诱导出愈伤组织，其中子叶和茎段较易诱导出愈伤组织；愈伤组织在MS + 1.0 mg?L-1 6-BA（或2.0 mg?L-1 KT）+ 0.2 mg?L-1 NAA培养基上进行继代培养效果较好，可获得质量较好的愈伤组织；在诱导愈伤组织再生培养中，通过不同激素配比处理外植体均未分化出不定芽，仅部分分化出不定根。
- Abstract:
- The hypocotyls,cotyledons,true leaves and stem segments of C.maxima ‘Beiguan’ inbred seedlings were used as explants,regenenration in vitro method was used to study the effects of different kinds of hormones on the development of callus in subculture,and to explore the regeneration of callus in vitro respectively for constructing high efficient regeneration system by callus way of pumpkin.The results showed that the callus could be induced from the hypocotyls,cotyledons,true leaves and stem segments of C.maxima ‘Beiguan’ inbred seedlings,and cotyledons and stem as explants were easier to induce callus than hypocotyls and true leaves.MS+1.0 mg?L-1 6-BA（or 2.0 mg?L-1 KT）+0.2 mg?L-1 NAA medium was better in callus subculture by obtaining better quality of the callus.In the induction of callus regeneration culture,none the adventitious bud was inducted from all callus explants with different hormone combinations,only part of the callus differentiated into adventitious roots.
参考文献/References:
[1]林德佩.南瓜植物的起源和分类[J].中国西瓜甜瓜,2000(1):36-38. [2]刘洋,屈淑平,崔崇士.南瓜营养品质与功能成分研究现状与展望[J].中国瓜菜,2006(2):27-29. [3]盛英,郭琪.南瓜多糖的提取及其药理作用研究概况[J].天津药学,2003(2):58-60. [4]黄黎慧,黄群,于美娟.南瓜的营养保健价值及产品开发[J].现代食品科技,2005(3):176-179. [5]李贞霞,李新峥,董卫华.南瓜组织培养体系建立研究[J].北方园艺,2005(3):75-76. [6]HAN J S.In vitro regeneration from cotyledon explants in figleaf gourd (Cucurbita ficifolia Bouche.),a rootstock for Cucurbitaceae[J].Plant Biotechnol Rep,2010（4）:101-107. [7]LEE Y K,CHUNG W,EZURA H.Efficient plant regeneration via organogenesis in winter squash(Cucurbita maxima Duch.)[J].Plant science,2003,164:413-418. [8]ANANTHAKRISHNAN G,XIA X,ELMAN C,et al.Shoot production in squash (Cucurbita pepo) by in vitro organogenesis[J].Plant Cell Reports,2003,21:739-746. [9]YOSHIHIKO N,YUTAKA T.Cucumber (Cucumis sativus L.) and Kabocha squash (Cucurbita moschata Duch)[J].Methods Mol Biol,2015,1223:299-310. [10]张玉园.南瓜子叶节离体再生体系的构建与耐盐StNHX1基因的转化[D].新乡:河南科技学院,2015. [11]张加一,刘霞,胡芳芳,等.南瓜子叶节再生体系的建立[J].中国瓜菜,2016(5):5-7,10. [12]CURUK S,ELMAN C,SCHLARMAN E,et al.A novel pathway for rapid shoot regeneration from the proximal zone of the hypocotyl of melon(Cucumis melon L.)[J].In Vitro Cellular & Developmental Biology-Plant,2002,38:260-267. [13]蒋素华,顾东亚,崔波,等.番茄真叶愈伤组织诱导及植株再生研究[J].北方园艺,2009(10):113-114. [14]吴秀华,张艳玲,周月,等.‘海沃德’猕猴桃叶片高频直接再生体系的建立[J].植物生理学报,2013(8):759-763. [15]高莉萍,包满珠.月季‘萨蔓莎’愈伤组织的诱导及植株再生[J].园艺学报,2005(3):534-536. [16]张卫华,孙小镭,王志峰,等.南瓜组织培养再生体系的研究[J].山东农业科学,2006(6):4-6. [17]邹克琴,张拥军,楼纪东,等.南瓜疏松愈伤组织诱导的研究[J].北方园艺,2007(12):201-203. [18]王爱玲,张敏,廖新福,等.甜瓜‘黄醉仙’下胚轴再生体系的初步建立[J].北方园艺,2013(12):100-103. [19]徐恒戬.KT和NAA对黑籽南瓜器官离体再生的影响[J].北方园艺,2007(4):200-202. [20]李代丽,康向阳.植物愈伤组织培养中内外源激素效应的研究现状与展望[J].生物技术通讯,2007(3):546-548. [21]MOHIUDDIN A K M,CHOWDHURY M K U,ABDULLAH Z C,et al.Influence of silver nitrate (ethylene inhibitor) on cucumber in vitro shoot regeneration[J].Plant Cell Tissue Organ Cult,1997,51:75-78. [22]赵巧阳,赖钟雄.硝酸银在离体培养和转化中的作用及其机理[J].亚热带农业研究,2008(1):62-66. [23]刘娟,汤浩茹,王小蓉,等.硝酸银在植物离体培养中的应用之研究进展[J].中国农学通报,2007(10):400-406. [24]张亚锋,曹家树,武涛.南瓜属植物再生体系的建立及其应用[J].植物生理学通讯,2007(3):599-604. [25]倪德祥.光在植物组织培养中的调控作用[J].自然杂志,1986(3):35-40. [26]〖JP2〗王维荣,王咏冬,欧阳光察,等.光质对黄瓜及番茄愈伤组织培养中分化和有关酶的影响[J].植物生理学报,1991(2):118-124. [27]CURUK S,ANANTHAKRISHNAN G,SINGER S,et al.Regeneration in vitro from the hypocotyl of Cucumis species produces almost exclusively diploid shoots,and does not require light[J].Hort Science,2003,38(1):105-109.
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备注/Memo
第一作者简介：鲁晓晓（1991-），女，硕士研究生，研究方向为蔬菜种质资源与育种。E-mail:luxiaoxiao914@163.com.责任作者：周俊国（1967-），男，博士，教授，现主要从事园艺植物育种与蔬菜栽培生理等研究工作。E-mail:junguo1020@163.com.基金项目：国家农业科技成果转化资金资助项目（2013GB2D000301）；河南科技学院2014年自然科学重大培育资助项目（ZD2014003）。收稿日期：2017-02-28