LI Chao,LI Hong,ZHANG Min.Formula Optimization of Auricularia auricula Liquid Spawn[J].Northern Horticulture,2017,41(16):170-173.[doi:10.11937/bfyy.20164845]
黑木耳液体培养基配方优化
- Title:
- Formula Optimization of Auricularia auricula Liquid Spawn
- Keywords:
- Auricularia auricula; liquid spawn; formula
- 文献标志码:
- A
- 摘要:
- 以“辽黑木耳1号”为试材，采用单因素试验和L9（34）正交优化实验，研究了不同碳源氮源和接种量对黑木耳菌丝生物量的影响，以期筛选出最佳黑木耳菌丝液体培养基配方。结果表明：黑木耳液体培养的最佳培养基配方为葡萄糖 2.0%，酵母膏 1.0%，磷酸二氢钾0.3%，硫酸镁0.15%，最佳接种量为10%，该条件下菌丝生物量可达12.3 g?L-1。
- Abstract:
- The single factor analysis and L9（34）orthogonal test were applied to get the best formula of Auricularia auricula liquid spawn.The results showed that the optimal composition of Auricularia auricula liquid spawn were as follows：glucose 2.0%，yeast extract 1.0%，KH2PO4 0.3%，MgSO4 0.15%.The optimal inoculation quantity of Auricularia auricula was 10%，in these conditions,the biomass could be achieved 12.3 g?L-1。
参考文献/References:
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备注/Memo
第一作者简介：李超（1976-），男，硕士，副研究员，研究方向为食用菌育种及栽培。E-mail：lnnkylxy@163.com.