CAI Yangxing,CAO Xiuming,LIN Dongmei,et al.Study on Polysaccharides in the Fruit Body of Hericium erinaceus Cultivated With JUNCAO[J].Northern Horticulture,2016,40(21):140-145.[doi:10.11937/bfyy.201621035]
菌草栽培猴头菌子实体多糖的研究
- Title:
- Study on Polysaccharides in the Fruit Body of Hericium erinaceus Cultivated With JUNCAO
- Keywords:
- Hericium erinaceus; polysaccharide; optimum condition of extraction; separation and purification
- 文献标志码:
- A
- 摘要:
- 以菌草栽培和木屑栽培的猴头菌子实体为试材，采用L9（34）正交实验设计方法对2种猴头菌子实体粗多糖进行提取，研究了不同浸提次数、料液比、浸提时间和温度对猴头菌子实体粗多糖得率的影响；用DEAE SepHarose Fast Flow离子柱层析法和SepHadex G-100凝胶柱层析色谱法对提取出的粗多糖进行初步分离和纯化，以期得到几个组分，且每组分为具有单一对称峰的多糖。结果表明：猴头菌子实体粗多糖提取的最佳条件是，浸提2次，料液比1∶25 g?mL-1，浸提时间4 h，温度90 ℃。菌草栽培猴头菌子实体多糖得到3个组分，分别记为cHEP1、cHEP2、cHEP3；木屑栽培猴头菌的子实体多糖纯化后只得到2个组分，分别记为sHEP1、sHEP2。对分离出的cHEP2、cHEP3和 sHEP2纯度鉴定结果表明，3种组分均显示为单一对称峰。
- Abstract:
- Using the fruit bodies of Hericium erinaceus which cultivated with JUNCAO and sawdust as test materials，the effect of coarse polysaccharide′s extraction rate from two kinds of fruit bodies on extraction condition such as extraction times，ratio of solid material to liquid，extraction time and temperature were investigated by L9（34）orthogonal experimental design method；the coarse polysaccharides were isolated and purified preliminary by DEAE SepHarose Fast Flow column chromatography method and SepHadex G-100 Gel chromatography method.Some components with single symmetrical peak were expected to obtain.The results showed that the optimize extract conditions of Hericium erinaceus fruit body′s polysaccharides were extract two times，ratio of material to water 1∶25 g?mL-1，time of 4 hours and temperature of 90 ℃.Three components were obtained from Hericium erinaceus fruit body′s polysaccharide under JUNCAO cultivation，there were cHEP1，cHEP2 and cHEP3；and only two components were abtained from the polysaccharide under traditional sawdust cultivation，there were sHEP1 and sHEP2.Purity identification showed that they were all single symmetrical peak.
参考文献/References:
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备注/Memo
第一作者简介：蔡杨星（1985-），男，硕士，助理实验师，现主要从事菌草技术研究及应用与推广的辅助教学和科研工作。E-mail：ccx5835963@163.com.责任作者：林占熺（1943-），男，研究员，博士生导师，菌草技术发明人，国家菌草工程技术研究中心首席科学家，现主要从事菌草技术研究与应用及菌草产业发展的教学与科研工作。E-mail：lzxjuncao@163.com.基金项目：福建省农业生物资源保藏资助项目（FJZZZY-1536）。