ZHANG Wanbo,PIAO Xuanchun,YANG Fan,et al.Effect of Plant Growth Regulators and Sucrose Concentrations on the Proliferation, Growth and Effective Compounds Content of Orostachys cartilaginous Callus[J].Northern Horticulture,2016,40(19):168-170.[doi:10.11937/bfyy.201619043]
植物生长调节剂和蔗糖浓度对狼爪瓦松愈伤组织生物量及有效物质积累的影响
- Title:
- Effect of Plant Growth Regulators and Sucrose Concentrations on the Proliferation, Growth and Effective Compounds Content of Orostachys cartilaginous Callus
- Keywords:
- Orostachys cartilaginous callus; MS medium concentration; plant growth regulators; sucrose concentrations; biomass; effective compounds
- 文献标志码:
- A
- 摘要:
- 以狼爪瓦松愈伤组织为试材,采用正交实验的方法,研究了MS基本培养基浓度及6-苄氨基腺嘌呤(6-BA)、萘乙酸(NAA)2种植物生长调节剂对狼爪瓦松愈伤组织生物量和有效物质含量的影响;同时,对适宜的蔗糖浓度进行了筛选。结果表明:MS+3.5 mg?L-1 6-BA+0.1 mg?L-1 NAA组合,最有利于愈伤组织生长和多糖、总酚及黄酮的积累;MS+3.5 mg?L-1 6-BA+0.1 mg?L-1 NAA培养基中加入30 g?L-1的蔗糖时,愈伤组织生长最佳,有效物质含量最高,在此蔗糖浓度下狼爪瓦松愈伤组织可生产711.2 mg?L-1多糖、165.1 mg?L-1总酚和154.5 mg?L-1黄酮。
- Abstract:
- Orostachys cartilaginous callus was used as experiment material,the effect of MS strength and concentrations of plant growth regulators,6-BA and NAA on callus biomass and effective compound accumulation was explored by orthogonal experiment method.The suitable sucrose concentration was also selected.The results showed that,MS medium supplemented with 3.5 mg?L-1 6-BA and 0.1 mg?L-1 NAA was favorable for callus growth and effective compound accumulation,the maxium fresh and dry weigth of calluses were found when the medium was supplied 30 g?L-1 sucrose,711.2 mg?L-1 of polysaccarides,165.1 mg?L-1 of phenolics and 154.5 mg?L-1 of flavonoids were obtained in Orostachys cartilaginous callus.
参考文献/References:
[1]吴征镒.中国植物志:第1卷[M].北京:科学出版社,2004. [2]李承范,王思宏,李东浩.狼爪瓦松提取物的抗氧化活性的研究[J].食品科技,2015,40(4):285-287. [3]尹秀梅,王思宏.长白山景天科植物品种、植物成分及酶抑制活性的研究进展[J].延边大学学报(自然科学版),2015,41(1):89-94. [4]郑万金,张萍,仲英.瓦松属植物的研究进展[J].齐鲁药事,2008,27(3):161-163. [5]李金蓉,丁遥,姜利健,等.MS和生长素浓度对大樱桃“明珠”组培苗生根的影响[J].延边大学农学学报,2015,37(2):123-126. [6]曲伟红,赵建国.瓦松愈伤组织诱导研究[J].时珍国医国药,2010,11(11):2832-2833. [7]李庆伟,梁明勤,樊亚敏,等.不同取材时期和处理方法对瓦松无菌体系建立的影响[J].江苏农业科学,2014,42(1):55-58. [8]陈丽娟,苏宾,闭志强,等.细胞分裂素6-BA浓度对红蕉组培快繁芽增殖的影响[J].广西农业科学,2001(2):67-68. [9]苏瑞军,邹利娟,吴庆贵,等.瓦松愈伤组织诱导及植株再生[J].中药材,2014,37(1):1-4. [10]李慧娟,高日,廉美兰,等.IBA、NAA和蔗糖浓度对东北刺人参不定根增殖生长的影响[J].中国农学通报,2011,27(5):281-283. [11]齐树亭,江莎.花卉克隆与栽培[M].北京:中国农业科学技术出版社,2004. [12]张静,李予霞,张霞.糖浓度对肉苁蓉(Cistanche deserticola)细胞系生长及甜菜碱积累的影响[J].种子,2010,29(4):44-45,49. [13]赵松峰.不同培养基及糖浓度对丰花月季叶愈伤组织诱导的影响[J].北方园艺,2009(4):56-58.
相似文献/References:
[1]邵元健,周蒋陈.一品红组培苗快繁技术的研究[J].北方园艺,2012,36(02):127.
SHAO Yuan-jian,ZHOU Jiang-chen.Study on the Tissue Culture Technology of the Seedling Propagation in Euphorbia pulcherrima Wild[J].Northern Horticulture,2012,36(19):127.
[2]高岳,朱旭东,司文会,等.微波灭菌对MS培养基的影响及效果[J].北方园艺,2012,36(23):129.
GAO Yue,ZHU Xu-dong,SI Wen-hui,et al.Study on Influence and Effect of Microwave Sterilization on MS Medium[J].Northern Horticulture,2012,36(19):129.
备注/Memo
第一作者简介:张万博(1991-),男,硕士研究生,研究方向为植物组织培养。E-mail:732635406@qq.com.责任作者:金英花(1982-),女,博士,讲师,现主要从事长白山经济植物生态特性与开发利用等研究工作。E-mail:yhuajin@ybu.edu.cn.基金项目:吉林省博士后科研资助项目(RB201307)。