XU Ruirui,CAO Cuiyu.Characteristics and Expression Analysis of Two CCCH Zinc Finger Genes SlC3H64 and SlC3H65 in Tomato[J].Northern Horticulture,2016,40(19):110-114.[doi:10.11937/bfyy.201619028]
番茄CCCH型锌指蛋白基因SlC3H64和SlC3H65的特征与表达分析
- Title:
- Characteristics and Expression Analysis of Two CCCH Zinc Finger Genes SlC3H64 and SlC3H65 in Tomato
- Keywords:
- tomato; CCCH zinc finger proteins; gene; tissues; stress treatment; expression analysis
- 文献标志码:
- A
- 摘要:
- 以番茄品种“中蔬6号”为试材，采用生物信息学方法和实时荧光定量PCR技术，分析研究了番茄SlC3H64和SlC3H65锌指蛋白结构特点，启动子序列内所含胁迫响应元件类别，以及2个基因组织表达模式和非生物胁迫条件下表达量变化差异。结果表明：番茄SlC3H64和SlC3H65的蛋白预测结构差异较大；启动子序列分析发现，2个基因中分别含有11个和7个不同的胁迫响应元件，且二者除了TC-rich repeat元件相同外，其余元件均不同；SlC3H64和SlC3H65在“中蔬6号”根、茎和叶中都有表达，且都在叶中表达量最高，5种不同胁迫处理条件下，SlC3H64响应水杨酸（SA）和高温处理，而SlC3H65则仅响应甘露醇处理。
- Abstract:
- Taking ‘Zhongshu No.6’ as test material，using the methods of bioinformatics and quantitative RT-PCR，protein structure of SlC3H64 and SlC3H65 CCCH zinc finger proteins were analyzed，several known stress responsive elements in the promoter of these two CCCH zinc finger genes were identified，the expression of these two CCCH zinc finger genes was analyzed in different vegetative organs and under five different stress treatments.The results showed that two CCCH zinc finger proteins had the almost completely different protein tertiary structures.Two CCCH zinc finger genes contained eleven and seven different stress response element，respectively.These elements were different between SlC3H64 and SlC3H65 CCCH zinc finger genes，except the TC-rich repeat element.The existence of stress-related elements in CCCH zinc finger gene promoter regions provided further evidence in future research.Expression analysis showed that two CCCH zinc finger genes expressed in root，stem and leaf in ‘Zhongshu No.6’，with the highest expression level in leaf，similarly.Under five different stress conditions，SlC3H64 was up-regulated under SA and high temperature stress treatment，while SlC3H65 only responsed to mannitol treatment.
参考文献/References:
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备注/Memo
第一作者简介：许瑞瑞（1982-），女，博士，副教授，研究方向为基因表达调控。E-mail:xuruirui2006@163.com.基金项目：山东省优秀中青年科学家科研奖励基金资助项目（BS2014SW014）；潍坊学院自然科学青年资助项目（2014Z12）。