ZHANG Xiaoyu,ZHOU Yonghui,LYU Ting,et al.Optimization of Culture Conditions for Morchella vulgaris Sclerotium From Huzhu′ Beishan Forest in Qinghai Province[J].Northern Horticulture,2016,40(18):146-148.[doi:10.11937/bfyy.201618036]
青海互助北山林场羊肚菌菌核培养条件的优化
- Title:
- Optimization of Culture Conditions for Morchella vulgaris Sclerotium From Huzhu′ Beishan Forest in Qinghai Province
- Keywords:
- Qinghai Province; Morchella vulgaris; sclerotium; mycelium; artificial culture
- 文献标志码:
- A
- 摘要:
- 以青海互助北山林场羊肚菌为试材，采用单因素变量法，研究不同的碳源、氮源、温度、pH对青海互助北山林场羊肚菌菌核数量及长势变化的影响。结果表明：当培养温度为22 ℃或26 ℃，pH介于6～7，并且分别以可溶性淀粉和硝酸钾为碳源和氮源培养基时，基面菌核和气生菌核数量最多，长势最好，说明上述培养条件是羊肚菌人工培养的最佳组合。
- Abstract:
- Taking Morchella vulgaris of Huzhu′ Beishan Forest in Qinghai Province as experimental material,the effect of different conditions such as carbon and nitrogen sources，temperature，pH on growing and number of Morchella vulgaris sclerotium was studied by using single factor variable method.The results showed that there were lots of datum sclerotium and aerial sclerotium and their growing was the best when the temperature was 22 ℃ or 26 ℃，pH was 6-7，soluble starch and KNO3 were regarded as carbon source and nitrogen source，respectively.Otherwise，the above culture condition should be the optimal choice.
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备注/Memo
第一作者简介：张晓宇（1992-），女，山西大同人，硕士研究生，研究方向为青藏高原植物资源的开发和利用。E-mail：zhangxiaoyu0918@163.com.责任作者：苏旭（1980-），男，山东鄄城人，博士，教授，研究方向为资源植物学。E-mail：xusu8527972@126.com.基金项目：青藏高原药用动植物资源重点实验室资助项目（2015-Z-Y06）；青海师范大学2015年度本科生科技创新资助项目（2015-12，2015-13）。