SHI Shouguo,HU Tingting.Cloning and Bioinformatics Analysis of the Jujube Fruit Flavonoid-3-hydroxylase Gene(F3H)[J].Northern Horticulture,2016,40(14):89-93.[doi:10.11937/bfyy.201614022]
枣果实黄烷酮-3-羟化酶基因（F3H）的克隆及生物信息学分析
- Title:
- Cloning and Bioinformatics Analysis of the Jujube Fruit Flavonoid-3-hydroxylase Gene(F3H)
- Keywords:
- anthocyanins; clone; semi-quantitative analysis; bioinformatics
- 文献标志码:
- A
- 摘要:
- 以枣果实为试材，采用同源克隆的方法，利用转录组测序结果设计F3H基因特异引物序列，成功克隆得到了枣果实F3H基因全长，对其进行了生物信息学分析，并对该基因含量变化与花色苷的关系进行了研究。结果表明：F3H基因全长1 336 bp，编码367个氨基酸残基。根据基因序列比对结果，枣果实F3H基因编码的氨基酸序列与龙眼（Dimocarpus longan）、橄榄（Canarium album）、荔枝（Litchi chinensis）及川桑（Morus notabilis）的一致性分别为90%、90%、89%、89%。半定量RT-PCR法分析表明，该基因在枣果实的红熟期表达量最大，其次为枣果实的半红期，而枣果实的绿熟期表达量最低；该基因表达量变化与花色苷含量变化一致。
- Abstract:
- Taking jujube fruit as material,adopting the homologous cloning method,based on transcriptome sequencing results,the jujube fruit F3H full-length gene was cloned.The bio-information analysis and the F3H influence on anthocyanins were studied.The results showed that the F3H gene was 1 336 bp,encoding 367 amino acid residues.According to the results of gene sequence alignment,sequence of amino acids of F3H gene had the consistency of 90%,90%,89% and 90% compared with Dimocarpus longan,Canarium album,Litchi chinensi and Morus notabilis,respectively.Semi-quantitative RT-PCR analysis showed that the gene expression in jujube fruit red ripe period was the most,followed by a half red jujube fruit,and jujube fruit green ripe stage expression quantity was minimum；extracting anthocyanins in jujube during different periods,the change of anthocyanins content in jujube,was consistent with gene expression quantity.
参考文献/References:
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备注/Memo
第一作者简介：师守国（1973-），男，山西运城人，博士，副教授，现主要从事园艺植物遗传育种与分子生物学等研究工作。E-mail：shishouguo2009@163.com.基金项目：运城学院博士启动经费资助项目（YQ-2014021）。