QIAN Changjiang,DU Yong,MU Jun,et al.Influence of GA3 and Temperature on Germination of Primula lithophila Seeds of Endemic Ornamental Plant of Guizhou[J].Northern Horticulture,2016,40(14):74-77.[doi:10.11937/bfyy.201614019]
赤霉素和温度对贵州特有观赏植物习水报春种子萌发的影响
- Title:
- Influence of GA3 and Temperature on Germination of Primula lithophila Seeds of Endemic Ornamental Plant of Guizhou
- Keywords:
- Primula lithophila; germination rate; germination potential; germination index; temperature; GA3 solution
- 文献标志码:
- A
- 摘要:
- 以习水报春种子为试验材料，采用不同温度梯度和20 ℃恒温下不同浓度赤霉素处理，研究了各种处理对习水报春种子的发芽率、发芽势和发芽指数的影响，以期找到最适萌发条件。结果表明：15 ℃处理发芽率（44.45%）最高；20 ℃处理发芽势（32.22%）最高和发芽指数（4.64）最高；25 ℃处理各项指标最低，发芽率1.11%，与10、15、20 ℃处理差异显著（P＜0.05），发芽势1.11%，与20 ℃处理差异显著（P＜0.05），发芽指数0.27，与20 ℃处理差异显著（P＜0.05）。最适萌发温度范围为15~20 ℃。在20 ℃恒温条件下，200 mg?L-1 GA3溶液处理发芽率（41.11%）最高；100、200 mg?L-1 GA3溶液处理发芽势（34.44%）最高，100 mg?L-1 GA3溶液处理发芽指数最高（5.53）。400、500 mg?L-1 GA3溶液处理与CK相比发芽率、发芽势差异均显著（P＜0.05），200、300、400、500 mg?L-1 GA3溶液处理与CK相比发芽指数差异显著（P＜0.05），GA3溶液的最适萌发浓度范围为100~200 mg?L-1。
- Abstract:
- The seeds of Primula lithophila were used as experimental material,with different temperature gradients and different concentrations of GA3 at 20 ℃ constant temperature to treat these seeds,and the influence of different treatments on germination rate，germination potential and germination index to find the optimal conditions for germination was studied.The results showed that the germination rate(44.45%) of 15 ℃ treatment was the highest;the germination potential(32.22%) and germination index(4.64) of 20 ℃ treatment was the highest.The every index of 25 ℃ treatment was the lowest，the germination rate(1.11%) of 25 ℃ treatment comparing with 10 ℃，15 ℃ and 20 ℃ treatment was significant difference(P<0.05).The germination potential(1.11%) of 25 ℃ treatment comparing with 20 ℃ treatment was significant difference(P<0.05)，and the germination index(0.27) of 25 ℃ treatment comparing with 20 ℃ treatment was significant difference(P<0.05).So the optimum germination temperature for these seeds was 15-20 ℃.At 20 ℃ constant temperature,the germination rate(41.11%) of 200 mg?L-1 GA3 solution treatment was the highest，and the germination potential(34.44%) of 100 mg?L-1 and 200 mg?L-1 GA3 solution treatment was the highest，and the germination index(5.53) of 100 mg?L-1 GA3 solution treatment was the highest.The control group(CK) treatment comparing with 400 mg?L-1 and 500 mg?L-1 GA3 solution treatment，the germination rate and germination potential was significant difference(P<0.05)，but the control group(CK) treatment comparing with 200 mg?L-1，300 mg?L-1，400 mg?L-1 and 500 mg?L-1 GA3 solution treatment，the germination index was significant difference(P<0.05).The optimum germination concentration of GA3 solution for these seeds was 100-200 mg?L-1.
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备注/Memo
第一作者简介：钱长江（1984-），男，博士研究生，副教授，研究方向为森林培育与植物分类和观赏植物资源。E-mail：qianchj520zh@163.com.基金项目：贵阳市乌当区科学技术计划资助项目（[2012]乌科技合同第54号）；贵州省科学技术厅科学技术基金资助项目（黔科合J字（2012）2287）；贵州省科学技术厅推广资助项目（黔科合字（2012）5033）；贵州省学位办生物学重点支持学科建设资助项目（2011231）。