ZHANG Lin,LI Xiangyu,LIN Yanping,et al.Research Progress of Carrot Genetic Transformation[J].Northern Horticulture,2016,40(13):201-204.[doi:10.11937/bfyy.201613051]
胡萝卜遗传转化研究进展
- Title:
- Research Progress of Carrot Genetic Transformation
- 文献标志码:
- A
- 摘要:
- 综述了胡萝卜遗传转化的研究进展，主要介绍了农杆菌介导法和DNA直接转化方法(包括基因枪法、电击法和PEG介导法)的分子机制和进展，并对遗传转化中受体基因型、外植体类型和菌株类型等影响因素进行阐述，以期为建立一个高效胡萝卜遗传转化体系提供理论依据。
- Abstract:
- The development of genetic transformation of carrot was reviewed in this research.This paper mainly introduced the molecular mechanisms and progress of the Agrobacterium-mediated methods and direct DNA transformation methods including particle bombardment，electroporation and PEG-mediated method.Some influencing factors in genetic transformation were described，for example，the receptor gene type，explant type，strain of the type and so on.It provided theoretical basis for the establishment of an efficient carrot genetic transformation system.
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备注/Memo
第一作者简介：张琳（1990-），女，硕士研究生，研究方向为功能基因组学。E-mail:1181351498@qq.com.责任作者：张美萍（1964-），女，博士，教授，博士生导师，现主要从事系统基因组学研究与应用等研究工作。E-mail:mpzhang@tamu.edu.基金项目：中国科学技术部863计划资助项目(2013AA102604-3)。