ZHOU Li,WANG Yuan,YE Yuanting,et al.Study on High Quality Plantlets Propagation Technique of Dendrobium officinale[J].Northern Horticulture,2016,40(10):95-99.[doi:10.11937/bfyy.201610024]
铁皮石斛优质试管苗繁育技术研究
- Title:
- Study on High Quality Plantlets Propagation Technique of Dendrobium officinale
- 文献标志码:
- A
- 摘要:
- 以野生铁皮石斛为试材，采用限根培养法，研究了铁皮石斛种子非共生萌发特性及优质试管苗繁育技术。结果表明：授粉120 d的种子达到生理成熟，能够顺利萌发；授粉140 d种子达到形态成熟，萌发率可达98.51%；低温黑暗限制保存原球茎可控制其生长；在特定容器中用限根培养基MS+0.10 mg?L-1 6-BA+0.3 mg?L-1 NAA+香蕉泥80 g?L-1 培养小苗可得到优质试管苗；把试管苗移栽到松树皮中，成活率可达97.62%，该研究为铁皮石斛产业化育苗提供了新方法。
- Abstract:
- Taking wild Dendrobium officinale as test material,using root restriction culture method，a high quality plantlets breeding technology of Dendrobium officinale was introduced by researching its characteristics of the asymbiotic seed germination.The results showed that the seeds of 120 days after pollination (DAP) reached maturity，the seeds could germinate；the seeds of 140 DAP could be morphologically mature，with a seed germination rate up to 98.51%；low temperature and darkness restriction could inhibit of protocorms growth；using medium of restrictions on root growth in a particular container could get high quality seedlings，restriction on root growth medium was MS+0.10 mg?L-1 6-BA+0.3 mg?L-1 NAA+80 g?L-1 mashed banana；and the survival rate could be up to 97.62% when the plantlets were transplanted to pine bark.Therefore，this research provided a new method for the industrial production of D.officinale
参考文献/References:
[1]中华人民共和国药典委员会．中华人民共和国药典（1部）[M]．北京：中国医药科技出版社，2010：265-266. [2]屠国昌．铁皮石斛的化学成分、药理作用和临床应用[J]．海峡药学，2010，22(2)：70-71. [3]翟明恬，刘红霞，王亚妮．铁皮石斛组织快繁及移栽培养[J]．东北林业大学学报，2015，43(1)：50-54. [4]杨立昌，乙引，张宇斌，等．铁皮石斛快速繁殖体系研究员研究[J]．北方园艺，2010(22)：136-138. [5]胡佳丽，戚正华，马美兰，等．铁皮石斛种子组织培养条件的优化[J]．浙江农业学报，2015，27(8)：1399-1402. [6]谢启鑫，宋小明，黄东华，等．铁皮石斛的种子培养[J]．北方园艺，2013(6)：90-91. [7]朱艳，秦民坚．铁皮石斛茎段诱导丛生芽的研究[J]．中国野生植物资源，2003，22(2)：56-57. [8]潘梅，王景飞，姜殿强，等．铁皮石斛丛生芽增殖培养条件的优化[J]．北方园艺，2013(13)：128-130. [9]张红梅，刘建东，王岩花，等．铁皮石斛茎段快繁技术研究会[J]．山西农业大学生学报(自然科学版)，2010，30(6)：495-499. [10]黄作喜，张杨，颜小玉，等．铁皮石斛原球茎高效增殖体系的构建[J]．北方园艺，2014(14)：107-110. [11]杨瑞娟，王桥美，严亮．铁皮石斛愈伤组织诱导研究[J]．亚热带植物科学，2014，43(3)：259-261.
相似文献/References:
[1]田英翠,曹受金,曹基武.六种榉树种子形态特征及萌发特性的研究[J].北方园艺,2014,38(11):71.
TIAN Ying-cui,CAO Shou-jin,CAO Ji-wu.Study on Morphology and Germination Characteristics of Seeds of Six Species of Zelkova schneideriana [J].Northern Horticulture,2014,38(10):71.
[2]杨 凯,孙迎坤,谭 雯,等.盐胁迫对香石竹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(22):86.
YANG Kai,SUN Ying-kun,TAN Wen,et al.Effects of Salt Stress on Seeds Germination and Seedling Growth of Carnation[J].Northern Horticulture,2013,37(10):86.
[3]马子林.藏药马尿泡种子吸水特性及抗旱性的初步研究[J].北方园艺,2013,37(22):164.
MA Zi-lin.Preliminary Study on Water Absorbing Character and Drought Resistance of the Seed of Tibetan Medicine Przewalskia tangutica Maxim[J].Northern Horticulture,2013,37(10):164.
[4]徐小玉,张凤银,张方俊.氯化钠胁迫对雏菊种子萌发及幼苗生长的影响[J].北方园艺,2014,38(14):52.
XU Xiao-yu,ZHANG Feng-yin,ZHANG Fang-jun.Effect of NaCl Stress on Seed Germination and Seedling Growth of Bellis perennis[J].Northern Horticulture,2014,38(10):52.
[5]韩志平,张海霞,刘 渊,等.NaCl胁迫对不同品种黄瓜种子萌发特性的影响[J].北方园艺,2014,38(01):1.
HAN Zhi-ping,ZHANG Hai-xia,LIU Yuan,et al.Effects of NaCl Stress on the Germination Characteristics of Different Varieties of Cucumber Seeds[J].Northern Horticulture,2014,38(10):1.
[6]黄作喜,张 杨,颜小玉,等.铁皮石斛原球茎高效增殖体系的构建[J].北方园艺,2014,38(14):107.
HUANG Zuo-xi,ZHANG Yang,YAN Xiao-yu,et al.Establishment for a Highly Efficient Proliferation System of Protocorm of Dendrobium officinale Kimura et Migo[J].Northern Horticulture,2014,38(10):107.
[7]张 芹,徐学山,李保会,等.不同处理条件对暴马丁香种子萌发的影响[J].北方园艺,2014,38(01):54.
ZHANG Qin,XU Xue-shan,LI Bao-hui,et al.Effects of Different Treatments Conditions on the Germination of Syringa reticulatassp. amurensisSeeds[J].Northern Horticulture,2014,38(10):54.
[8]潘梅,王景飞,姜殿强,等.铁皮石斛丛生芽增殖培养条件的优化[J].北方园艺,2013,37(13):128.
PAN Mei,WANG Jing-fei,JIANG Dian-qiang,et al.Clustered Buds Proliferation Optimization of Culture Conditions of Dendrobium candidum[J].Northern Horticulture,2013,37(10):128.
[9]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(10):148.
[10]崔霞,梁燕,王玲慧.不同浓度化学诱变剂甲基磺酸乙酯对番茄种子发芽的影响[J].北方园艺,2013,37(14):28.
CUI Xia,LIANG Yan,WANG Ling-hui.Effect of Different Concentrations of Chemical Mutagen EMS on Tomato Seed Germination[J].Northern Horticulture,2013,37(10):28.
备注/Memo
第一作者简介：周丽(1978-)，女，贵州兴义人，硕士，副教授，现主要从事植物组织培养及植物资源保育与开发利用等研究工作。E-mail：zhouli@xynun.edu.cn. 基金项目：贵州省优秀科技教育人才省长专项资金资助项目（黔省专合字（2012）86号）；贵州省教育厅2011年教育质量提升资助项目(黔教高发[2011]278号)；贵州省教育厅教育创新团队资助项目（黔教合人才团队[2013]30号）。