FANG Zhongming,BAI Genxiang,ZENG Qisen,et al.Effect of Natural Additives on Protocorm Proliferation，Differentiation and Rooting of Dendrobium officinale[J].Northern Horticulture,2015,39(21):107-110.[doi:10.11937/bfyy.201521028]
天然添加物对铁皮石斛类原球茎增殖、分化及生根的影响
- Title:
- Effect of Natural Additives on Protocorm Proliferation，Differentiation and Rooting of Dendrobium officinale
- Keywords:
- :Dendrobium officinale; tissue culture; natural additives
- 文献标志码:
- A
- 摘要:
- 以云南软脚铁皮石斛为试材，研究了不同天然添加物对其类原球茎增殖、分化及小苗生根的影响。结果表明：高浓度的香蕉汁抑制类原球茎的增殖，而高浓度的马铃薯汁促进类原球茎的增殖，铁皮石斛类原球茎增殖最适培养基为MS+6-BA 1 mg/L+NAA 1 mg/L+蔗糖20 g/L+琼脂8 g/L+活性炭2 g/L+马铃薯汁200 g/L，接种60 d后类原球茎增殖倍数为6.8，继续培养30 d分化率达97%。培养基中添加香蕉汁、马铃薯汁均有利于铁皮石斛小苗生根，最适生根的天然添加物为马铃薯汁100 g/L。
- Abstract:
- Taking Yunnan Ruanjiao D.officinale as metarials，the effect of natural additives on protocorms proliferation，differentiation and rooting were studied.The results showed that high concentrations of banana juice could inhibit the proliferation of protocorm of D.officinale but banana juice was favorable to proliferation.The best protocorm proliferation medium of D.officinale was MS+6-BA 1 mg/L+NAA 1 mg/L+sucrose 20 g/L+agar 8 g/L+activated carbon 2 g/L+potato juice 200 g/L，with multiplication rate 6.8 after 60 days，and differentiation rate reached to 97% after 30 days.When adding banana，or potato juice in MS，it had an obvious effect on rooting.The optimal natural additives for rooting was potato juice 100 g/L.
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备注/Memo
第一作者简介：方中明(1984-)，男，博士，讲师，研究方向为植物生物技术。E-mail：zmfang@mail.hzau.edu.cn.责任作者：曾宋君(1965-)，男，博士，研究员，研究方向为植物生物技术。E-mail：zengsongjun@scib.ac.cn.基金项目：国家自然科学基金资助项目（31301250）;湖北省教育厅科学技术研究资助项目(B2015394)。