LIU Tailin,LI Li,ZHOU Zhengtao,et al.Selection of Solid Media Formulations for Six Armillaria mellea[J].Northern Horticulture,2015,39(18):145-148.[doi:10.11937/bfyy.201518039]
六种蜜环菌固体培养基配方的筛选
- Title:
- Selection of Solid Media Formulations for Six Armillaria mellea
- Keywords:
- Armillaria mellea; strain; solid medium; selection
- 文献标志码:
- A
- 摘要:
- 引种了6个蜜环菌菌种分别接种于8个不同配方的固体培养基中进行筛选试验。从萌发时间、满皿时间、生长势、菌索干重等方面进行评价。结果表明：菌种AM7在培养基配方8（马铃薯20 g，木屑20 g，麦麸20 g，胡萝卜20 g，蛋白胨5 g，牛肉膏5 g，葡萄糖20 g，琼脂10 g，KH2PO4 2 g，MgSO4 1 g，维生素B110 mg，水1 000 mL）上萌发时间最短，生长速度快，生长生物量最大，与其它菌种和配方组合差异达显著或极显著水平，是最佳组合；菌种AM6和宁强A9菌种在配方8培养基上生长生物量较高，与其它组合差异显著，也是较好的菌种。该研究筛选出的优良品种和配方组合可以用于生产。
- Abstract:
- Six Armillaria mellea strains were introduced to culture in eight different solid media for selection.The time of germination and overgrow of utensil，the growth potential and the shoestring dry weight were evaluated.The results showed that the best combination was the AM7 strain cultured in medium 8 (potato 20 g，sawdust 20 g，wheat bran 20 g，carrot 20 g，peptone 5 g，beef extract 5 g，glucose 20 g，agar 10 g，KH2PO4 2 g，MgSO4 1 g，vitamin B110 mg，ddH2O 1 000 mL)，which had the shortest germination，fast growth，the largest growth biomass.There was a significant or extremely significant difference with other combinations.The bacteria strains AM6 and Ning Qiang A9 in the formula 8 medium had a significant difference with other combinations，which were also good strains.The good species and formula combination selected by this study can be used for production.
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备注/Memo
第一作者简介：刘太林(1983-)，男，硕士，讲师，现主要从事药用植物栽培育种与生物技术等研究工作。E-mail：huajizi83@126.com. 基金项目：天津天狮学院校内资助项目（K13005）。