WANG Qian,GUO Miao,LIU Min,et al.A Method Employ Waste Material of Nejayote as Raw Material to Prepare Pleurotus nebrodensia Liquid Spawn[J].Northern Horticulture,2015,39(09):115-117.[doi:10.11937/bfyy.201509032]
一种以黄浆水为原料制备白灵菇液体菌种的方法
- Title:
- A Method Employ Waste Material of Nejayote as Raw Material to Prepare Pleurotus nebrodensia Liquid Spawn
- 文章编号:
- 1001-0009（2015）09-0115-03
- Keywords:
- Pleurotus nebrodensia; nejayote; liquid spawn
- 分类号:
- S 646.1+ 9
- 文献标志码:
- A
- 摘要:
- 以黄浆水为试材，采用正交实验法和单因素试验法，研究液体培养基和培养条件对制备白灵菇液体菌种的影响，并进行了液体菌种的应用效果比较。结果表明：白灵菇液体菌种明显优于固体菌种，以黄浆水为原料的白灵菇液体菌种的最佳培养基配方为葡萄糖3%，豆饼粉0.6%，MgSO4?7H2O 0.1%，KH2PO4 0.1%，维生素B1 5 mg/L，黄浆水补足100%；最佳工艺条件为初始pH 6.5，接种量10%，摇床转速180 r/min。
- Abstract:
- Taking waste of nejayote as test material,the effect of liquid medium and culture conditions on the preparation of Pleurotus nebrodensia liquid spawn were studied by orthogonal and the single-factor test,liquid spawn application was compared.The results showed that Pleurotus nebrodensis liquid spawn was significantly better than the solid strains，the best medium for Pleurotus nebrodensia liquid spawn using waste of nejayote as raw material was glucose 3%，soybean meal 0.6%，vitamin B1 5 mg/L，MgSO4?7H2O 0.1%，KH2PO4 0.1%,nejayote making up 100%；the best Pleurotus nebrodensia fermentation conditions were initial pH 6.5，inoculum 10%，shaking speed 180 r/min.
参考文献/References:
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备注/Memo
第一作者简介：王谦（1962-），男，本科，研究员，现主要从事食药用真菌研究与开发等研究工作。E-mail：wq6203_cn@126.com.