ZHANG Xiao-mei,ZHU Wei-hong,JIA Chun feng.Culture Characteristic Comparison and Condition Control of Fermentation of Two Polysaccharides Agrocybe chaxingu[J].Northern Horticulture,2014,38(23):127-129.
二株茶薪菇菌丝多糖深层发酵的培养特征比较与条件控制
- Title:
- Culture Characteristic Comparison and Condition Control of Fermentation of Two Polysaccharides Agrocybe chaxingu
- 文章编号:
- 1001-0009(2014)23-0127-03
- Keywords:
- Agrocybe chaxingu; submerged fermentation; polysaccharide; culture characteristic; compare
- 分类号:
- S 646.9
- 文献标志码:
- A
- 摘要:
- 以2株茶薪菇为试材，研究比较了其多糖深层发酵的培养特征及发酵的控制条件。结果表明：茶薪菇多糖发酵罐深层培养以26℃，pH 6.5，150～250 r/min搅拌，0.03～0.05 MPa罐压，0.2～0.4 m3/h通风，发酵96 h较适宜；菌丝胞内多糖达937～1 080 mg/g。2个菌株罐批培养多糖高峰期与摇瓶一致，但产量略高，较固体栽培子实体多糖高1.3～1.5倍，时间缩短12倍。比较发现，菌株U27厚垣孢子数量较多，液体培养效果较好，摇瓶种子菌球小而齐，直径1.0～2.0 mm，密度（1.5～2.5）×103个/mL，液体深层发酵的菌丝多糖达1 080 mg/g，为子实体多糖的1.5倍，是1株良好的多糖深层发酵的生产菌株。
- Abstract:
- Taking two samples of polysaccharides Agrocybe chaxingu as material,the culture characteristic and control condition of fermentation were compared.The results showed that a suitable condition of 26℃，pH 6.5，150-250 r/min stir，0.03-0.05 MPa pressure，0.2-0.4 m3/h airflow，and a duration of 96 hours to produce 937-1 080 mg/g of polysaccharide.Tank batch culture shared the same peak period of polysaccharide as that of bottle shaking，but yielded slightly higher.Compared against solid cultivation of fruiting bodies，they yielded 1.3-1.5 times more polysaccharide，and the duration has been shortened by 12-fold.By comparison，the U27 had more chlamydospore，and was more suitable for liquid culture was discovered.By bottle shaking，the fungus balls were small and neat，with diameters between 1.0 and 2.0 mm，density between (1.5-2.5)×103/mL.The submerged fermentation in liquid yielded up to 1 080 mg/g polysaccharide，which was 50% more than that of fruiting，and was proved to be a healthy choice of polysaccharide submerged fermentation.
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备注/Memo
第一作者简介：张筱梅(1957-)，女，教授，研究方向为食药用真菌应用。E-mail：zhxm06@163.com.