ZHANG Nan,LI Yong,XU Jie,et al.Medium Optimization for Cordycepin Production From Cordyceps militaris by Submerged Fermentation[J].Northern Horticulture,2017,41(05):134-141.[doi:10.11937/bfyy.201705032]
蛹虫草深层发酵产虫草素培养基的优化
- Title:
- Medium Optimization for Cordycepin Production From Cordyceps militaris by Submerged Fermentation
- Keywords:
- Cordyceps militari; submerged fermentation; cordycepin; mycelial growth; medium; optimization
- 文献标志码:
- A
- 摘要:
- 以蛹虫草(Cordyceps militaris (L.) Link)NS-810为菌种，通过对接种量的考察，探索不同孢子浓度对蛹虫草液体一级种制作效果的影响；通过单次单因子试验和正交实验，优化深层发酵产虫草素的最佳培养基，筛选制备蛹虫草液体一级种和液体发酵生产虫草素的最佳工艺。结果表明：孢子浓度3.0×108 cfu?mL-1时制作的母种最适合作为蛹虫草菌种扩大培养中的一级种子液；深层发酵的最佳培养基配方为葡萄糖25 g?L-1、土豆100 g?L-1、鱼蛋白胨18 g?L-1、(NH4)2SO4 0.8 g?L-1、KH2PO4 1.0 g?L-1、MgSO4?7H2O 0.5 g?L-1、蚕蛹粉5.0 g?L-1、维生素B1 18 mg?L-1、水1 L。优化后虫草素的总产量为1 144.31 mg?L-1，较基础培养基提高了1.46倍。分别以价格低廉葡萄糖和鱼蛋白胨作为发酵培养基的碳源和有机氮源，利于蛹虫草产业化发酵生产虫草素。
- Abstract:
- In order to explore the optimal preparation method of liquid seed and medium for cordycepin production in submerged cultivation of Cordyceps militaris (L.) Link NS-810，effect of liquid seed on mycelial growth by inoculating different concentrations of spores in seed culture was investigated.Single factor experiment and orthogonal experiment were used to select the optimal medium compositions for cordycepin production in liquid fermentation.The results showed that the best concentration of spores for first-degree spawn was 3.0×108 cfu?mL-1.Under this condition，the pale yellow hypha ball dispersed homogeneously in the seed liquid and their diameters were in the range of 1.1 mm to 1.3 mm，and the seed liquid was clear and transparent.Furthermore，the optimal media was composed of the following factors:glucose 25 g?L-1，potato 100 g?L-1，peptone (fish) 18 g?L-1，(NH4)2SO4 0.8 g?L-1，KH2PO4 1.0 g?L-1，MgSO4?7H2O 0.5 g?L-1，silkworm pupa powder 5.0 g?L-1，water 1 L.This optimization strategy enhanced the production of cordycepin nearly 1.46 times compared with the initial level.Low-cost glucose and peptone (fish) were used as quick-release carbon source and organic nitrogen source of fermentation medium respectively，which was beneficial for industrial production of cordycepin from the strain NS-810 by submerged fermentation.
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备注/Memo
第一作者简介：张楠（1980-），男，博士，副教授，研究方向为微生物学与食用菌种植。E-mail:nan25000@163.com.责任作者：段辉国(1967-)，男，硕士，教授，硕士生导师，研究方向为生物化学。E-mail:duanhuiguo6@163.com.基金项目：四川省教育厅重点资助项目（11ZA029）；川西珍稀植物资源研究与利用创新团队资助项目（14TD0025）。