REN Hai-long,LI Wen-hui,XU Lin,et al.The Seperation and Bacteria 16S rDNA Identification of Tomato Ralstonia solanacearum in Off-season Breed Area[J].Northern Horticulture,2013,37(02):80-82.
南繁基地番茄青枯菌的分离与16S rDNA鉴定
- Title:
- The Seperation and Bacteria 16S rDNA Identification of Tomato Ralstonia solanacearum in Off-season Breed Area
- 文章编号:
- 1001-0009(2013)02-0080-03
- Keywords:
- off-season bread area; tomato; Ralstonia solanacearum; 16S rDNA
- 分类号:
- Q 949.3
- 文献标志码:
- A
- 摘要:
- 对新疆农业科学院南繁试验基地的得青枯病的番茄植株进行病原菌分离，采用细菌16S rDNA测序方法进行鉴定。结果表明：鉴定出致病菌为番茄青枯菌（Ralstonia solanacearum），并探讨了南繁番茄青枯病的防治策略。
- Abstract:
- Pathogens was separated from the disease tomato plants，which came from off-season bread area of Xinjiang Academy of Agricultural Sciences，and it was indentified by the method of bacteria 16S rDNA.The results showed that the pathogen was Ralstonia solanacearum，and the strategies of prevention and treatment for tomato bacterial wilt in off-season bread area were discussed.
参考文献/References:
[1]张春奇,查素娥,李红波.番茄育种研究概况及展望[J].农业科技通讯,2011(3):29-33. [2]肖雷,余庆辉.南繁基地加工番茄的栽培技术[J].农村科技,2006(2):30-31. [3]林明宝,汪国平,卢婷,等.番茄抗青枯病材料及抗病基因SSR标记的初步筛选[J].安徽农业科学,2008,36(9):3538-3539. [4]刘达凤,陈须文,汪金莲.番茄不同品种抗青枯病研究[J].江西农业大学学报,1996,18(3):360-362. [5]Hauward A C.Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum[J].Annu Rev Phytopathol,1991,29:65-87. [6]汪国平,袁四清,熊正葵,等.广东省番茄青枯病相关研究概况[J].广东农业科学,2003(3):32-34. [7]佘小漫,何自福,李华平,等.广东茄科雷尔氏菌菌株生化变种的测定[A].中国植物病理学会2008年学术年会论文集[C].2008:337-339. [8]Chen D，Zhang Q，Q Ian K C，et al.Sequence analysis of 16S rRNA gene from 3 microcystis flos-aquae aeruginosa in Guangdong Reservoirs[J].Ecologic Science,2006,25(1):41-42. [9]杨雪颖,张执欣,杨亚珍,等.甘草根瘤菌的16S rDNA全序列测定及系统进化分析[J].西北植物学报,2006,26(4):707-711. [10]佘小漫,何自福,虞皓,等.广东茄科雷尔氏菌16S rDNA序列分析[J].华南农业大学学报,2009，30(4):24-28. [11]邹庆道,朱华,张子君,等.番茄抗青枯病材料的筛选[J].北方园艺,2011(2):28-29. [12]寿森炎,冯壮志,苗立祥,等.番茄抗青枯病基因的AFLP分子标记[J].遗传,2006,28(2):195-199.
相似文献/References:
[1]吴晓燕,张丽荣,马建华,等.一种新型微生物制剂对设施番茄土壤微生物数量的影响[J].北方园艺,2013,37(04):42.
[2]刘配军,李景富,许向阳,等.番茄hp1和dg基因高效检测体系的建立[J].北方园艺,2013,37(04):99.
[3]王晓娥,李建国,赵姝荣,等.咸阳市设施番茄根结线虫病的发生与防治[J].北方园艺,2013,37(04):133.
[4]谷婧玥,李景富,许向阳,等.番茄不同果穗间品质性状差异性研究[J].北方园艺,2014,38(12):26.
GU Jing-yue,LI Jing-fu,XU Xiang-yang,et al.Study on Tomato’s Quality and Traits at Different Tassel[J].Northern Horticulture,2014,38(02):26.
[5]吕 涛,于 杰,赵 勇.日光温室越冬栽培番茄适宜品种筛选试验[J].北方园艺,2014,38(12):40.
LV Tao,YU Jie,ZHAO Yong.Selection of Overwintering Tomato Varieties Suitable for Solar Greenhouse in Heilongjiang[J].Northern Horticulture,2014,38(02):40.
[6]李美芹,裴华丽,乔 宁,等.TYLCV外壳蛋白基因植物表达载体构建及转化番茄的研究[J].北方园艺,2014,38(12):84.
LI Mei-qin,PEI Hua-li,QIAO Ning,et al.Study on the Construction of Plant Expression Vector of TYLCV-CP Gene and Its Transferring Into Tomato[J].Northern Horticulture,2014,38(02):84.
[7]李文枫,李景富,许向阳.不同hp基因类型番茄中番茄红素与主要品质性状的差异及相关分析[J].北方园艺,2014,38(13):97.
LI Wen-feng,LI Jing-fu,XU Xiang-yang.Correlation and Difference Analysis Between Lycopene Content and Main Quality Characters of Tomato with Different hp Gene Types[J].Northern Horticulture,2014,38(02):97.
[8]张 浩,李福云,徐志然,等.不同套作模式对温室连作番茄生长产量及土壤微生物与酶活性的影响[J].北方园艺,2014,38(13):46.
ZHANG Hao,LI Fu-yun,XU Zhi-ran,et al.Effects of Different Relay Intercropping Patterns on Continuous Cropping Tomato Growth，Yield and Soil Microflora and Enzymes Activities in Solar Greenhouse[J].Northern Horticulture,2014,38(02):46.
[9]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(02):158.
[10]祁连弟,管建慧,其日格,等.不同比例牛羊粪便栽培基质对温室番茄苗期生长的影响[J].北方园艺,2013,37(22):47.
QI Lian-di,GUAN Jian-hui,QI Ri-ge,et al.Effects of Different Nursery Substrates with Cow and Sheep Dung on the Seedlings Growth of Tomato in Solar Greenhouse[J].Northern Horticulture,2013,37(02):47.
备注/Memo
第一作者简介：任海龙（1985-），男，硕士，助理研究员，研究方向为作物抗病遗传育种。E-mail:renhailong_2006@163.com.