[1]陈翠翠,兰阿峰,郭素芬,等.连作羊肚菌对土壤性质和微生物群落的影响[J].北方园艺,2026,(10):88-98.[doi:10.11937/bfyy.20260097]
　CHEN Cuicui,LAN Afeng,GUO Sufen,et al.Effects of Continuous Cropping of Morchella on Soil Properties and Microbial Communities[J].Northern Horticulture,2026,(10):88-98.[doi:10.11937/bfyy.20260097]
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连作羊肚菌对土壤性质和微生物群落的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年10 页码: 88-98 栏目: 出版日期: 2026-05-30
Title:
Effects of Continuous Cropping of Morchella on Soil Properties and Microbial Communities
文章编号:
1001-0009(2026)10-0088-11
作者:
陈翠翠1; 兰阿峰1; 2; 3; 郭素芬1; 3; 曹乐1; 彭浩1; 2; 3
（1.陕西理工大学 生物科学与工程学院，陕西 汉中 723001；2.陕西理工大学 秦巴生物资源与生态环境省部共建国家重点实验室（培育），陕西 汉中 723001；3.陕西省资源生物重点实验室，陕西 汉中 723001）
Author(s):
CHEN Cuicui1; LAN Afeng1; 2; 3; GUO Sufen1; 3; CAO Le1; PENG Hao1; 2; 3
(1.College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723001；2.Qinba State Key Laboratory of Biological Resources and Ecological Environment,Shaanxi University of Technology,Hanzhong,Shaanxi 723001;3.Shaanxi Province Key Laboratory of Bio-resources,Hanzhong,Shaanxi 723001)
关键词:
连作羊肚菌; 高通量测序; 土壤性质; 微生物群落
Keywords:
continuous cropping of Morchella; high-throughput sequencing; soil properties; microbial communities
分类号:
S646;S154.3
DOI:
10.11937/bfyy.20260097
文献标志码:
A
摘要:
以未种植过羊肚菌的土壤（CK）及羊肚菌连作3年（Y3）、6年（Y6）的根际土壤为试材，采用常规分析和高通量测序方法，研究了连作羊肚菌对土壤性质和微生物群落的影响，以期为缓解羊肚菌连作障碍提供参考依据。结果表明：随着连作年限的增加，羊肚菌连作土壤中速效磷和速效钾显著增加，碱解氮含量和电导率先增加后降低，其他理化性质变化不显著。与对照相比，土壤中细菌群落丰富度和多样性随连作年限延长先下降后增加，真菌群落丰富度和多样性随连作年限延长呈下降趋势。连作导致有益菌门如放线菌门Actinobacteriota、硝化螺旋菌门Nitrospirota、芽单胞菌门Gemmatimonadota等丰度显著降低，硝化螺旋菌属Nitrospira、芽孢杆菌属Bacillus、慢生根瘤菌属Bradyrhizobium、芽单胞菌属Gemmatimonas等丰度显著降低。连作导致担子菌门Basidiomycota丰度降低，被孢霉门Mortierellomycota丰度增加，子囊菌门Ascomycota趋向于最优势菌群，Pseudopithomyces、暗梗单孢霉属Chloridium、木霉属Trichodcrma、Saitozyma丰度降低。相关性分析表明，细菌群落结构易受SWC(P＜0.05)和AK影响，而真菌群落结构易受土壤EC值(P＜0.05)和pH的影响。综上，连作导致羊肚菌土壤性质改变，微生物群落结构失衡，微生物群落受土壤性质影响较大。
Abstract:
Taking soil samples from uncultivated M.sextelata (CK) and rhizosphere soils of continuous cropping for 3 years (Y3) and 6 years (Y6) as the test materials,the effects of continuous M.sextelata cropping on soil properties and microbial communities were studied by using conventional analytical methods and high-throughput sequencing technology,in order to provide a reference for alleviating continuous cropping obstacles in M.sextelata cultivation.The results showed that with increasing years of continuous cropping,available phosphorus and potassium in M.sextelata cropping soils significantly increased,while alkaline-hydrolyzable nitrogen content and electrical conductivity first increased then decreased.Other physicochemical properties showed no significant changes.Compared with the control,bacterial community richness and diversity in soil first decreased then increased with longer continuous cropping periods,while fungal community richness and diversity showed a declining trend over time.Continuous cropping significantly reduced the abundance of beneficial bacterial phyla such as Actinobacteriota,Nitrospirota,and Gemmatimonadota,while the abundance of Nitrospira,Bacillus,Bradyrhizobium,and Gemmatimonas.Continuous cropping deecreased the abundance of Basidiomycota and increased that of Mortierellomycota,making Ascomycota the dominant fungal group.The abundance of Pseudopithomyces,Chloridium,Trichoderma,and Saitozyma decreased.Correlation analysis indicated that bacterial community structure was significantly influenced by soil water content (P＜0.05),available potassium (AK).Fungal community structure was significantly affected by electrical conductivity value (P＜0.05),pH.In conclusion,continuous cropping resulted in alterations in rhizosphere soil properties,disruption of microbial community structure,and significant influence of soil properties on microbial communities.

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备注/Memo

第一作者简介：陈翠翠（2001-），女，硕士研究生，研究方向为微生物资源保育及开发应用。E-mail:15991885433@163.com.责任作者：兰阿峰(1978-)，男，博士，讲师，现主要从事微生物多样性等研究工作。E-mail:lanafeng@sunt.edu.cn.基金项目：陕西省重点研发计划资助项目(2023-YBNY-091)；陕西省教育厅科技计划资助项目(20JS024)。收稿日期：2026-01-09

更新日期/Last Update: 2026-06-04