[1]周梓晗,杨建帮,沙凤霞,等.两个枸杞品种不同器官褪黑素含量及其合成酶基因表达[J].北方园艺,2026,(6):27-32.[doi:10.11937/bfyy.20253384]
　ZHOU Zihan,YANG Jianbang,SHA Fengxia,et al.Melatonin Content and Expression of Biosynthetic Gene in Different Organs of Two Lycium barbarum L. Cultivars[J].Northern Horticulture,2026,(6):27-32.[doi:10.11937/bfyy.20253384]
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两个枸杞品种不同器官褪黑素含量及其合成酶基因表达

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2026年6 页码: 27-32 栏目: 出版日期: 2026-03-30
Title:
Melatonin Content and Expression of Biosynthetic Gene in Different Organs of Two Lycium barbarum L. Cultivars
文章编号:
1001-0009(2026)06-0027-06
作者:
周梓晗1; 杨建帮2; 沙凤霞1; 姚文孔1; 3; 张宁1; 3; 冯美1; 3
（1.宁夏大学 葡萄酒与园艺学院,宁夏 银川 750021；2.中宁县喊叫水乡人民政府,宁夏 中卫 755107；3.农业农村部园艺作物节水抗逆高效生产创新重点实验室,宁夏 银川 750021）
Author(s):
ZHOU Zihan1; YANG Jianbang2; SHA Fengxia1; YAO Wenkong1; 3; ZHANG Ning1; 3; FENG Mei1; 3
(1.College of Wine and Horticulture，Ningxia University，Yinchuan，Ningxia 750021；2.Zhongning County Hanjiao Water Township People′s Government，Zhongwei，Ningxia 755107；3.Key Laboratory of Innovation in Water-saving and Stress-resistant Efficient Production of Horticultural Crops，Ministry of Agriculture and Rural Affairs，Yinchuan，Ningxia 750021)
关键词:
枸杞; 褪黑素含量; 基因表达; 褪黑素合成酶
Keywords:
Lycium barbarum L.; melatonin content; gene expression; melatonin biosynthetic enzymes
分类号:
S567.19
DOI:
10.11937/bfyy.20253384
文献标志码:
A
摘要:
以‘宁杞1号’‘宁杞菜1号’夏季的根、茎、叶、花鲜样为试材，采用高效液相色谱法及荧光定量PCR技术，研究了不同器官褪黑素含量及合成酶基因表达量，以期为后续宁夏枸杞褪黑素合成研究及药用价值挖掘提供参考依据。结果表明：‘宁杞菜1号’各器官褪黑素含量显著高于‘宁杞1号’，2个枸杞品种褪黑素含量均表现为花＞叶＞茎＞根含量。TDC、T5H、COMT00、COMT01这4种酶基因在根、茎、叶、花中均有表达。
Abstract:
Taking the fresh roots，stems，leaves，and flowers of ‘Ningqi 1’ and ‘Ningqicai 1’ sampled in summer as the experimental materials,high-performance liquid chromatography (HPLC) and quantitative real-time polymerase chain reaction (qRT-PCR) were utilized to investigate melatonin contents and the expression profiles of melatonin biosynthetic genes across different organs，in order to provide reference for the subse quent research on the synthesis of melationin in Lycium barbarum L.and the exploration of its medicinal value.The results showed that the melatonin content in each organ of ‘Ningqicai 1’ was significantly higher than that of ‘Ningqi 1’.For both cultivars，the melatonin content followed the descending order of flower>leaf>stem>root.Additionally，the four biosynthetic genes，namely TDC,T5H,COMT00，and COMT01，were ubiquitously expressed in roots，stems，leaves，and flowers.

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备注/Memo

第一作者简介：周梓晗（1997-），女，硕士研究生，研究方向为果树学。E-mail:15052642926@163.com.责任作者：冯美（1970-），女，硕士，教授，硕士生导师，现主要从事果树学等研究工作。E-mail：feng_m@nxu.edu.cn.基金项目：宁夏自然科学基金资助项目(2024AAC02026)；国家自然科学基金资助项目（3236140164）。收稿日期：2025-09-23

更新日期/Last Update: 2026-04-01