[1]张倩,任海林,王洁,等.猕猴桃FBA基因家族的鉴定及在干旱胁迫下的表达模式分析[J].北方园艺,2025,(23):20-29.[doi:10.11937/bfyy.20251039]
　ZHANG Qian,REN Hailin,WANG Jie,et al.Identification of the FBA Gene Family in Actinidia chinensis and Analysis of Its Expression Pattern Under Drought Stress[J].Northern Horticulture,2025,(23):20-29.[doi:10.11937/bfyy.20251039]
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猕猴桃FBA基因家族的鉴定及在干旱胁迫下的表达模式分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年23 页码: 20-29 栏目: 出版日期: 2025-12-15
Title:
Identification of the FBA Gene Family in Actinidia chinensis and Analysis of Its Expression Pattern Under Drought Stress
文章编号:
1001-0009(2025)23-0020-10
作者:
张倩1; 任海林1; 王洁1; 聂莉莉1; 王浩宇1; 张汉尧1; 2
（1.西南林业大学 云南省林内资源保护与利用重点实验室，云南 昆明 650224;2.西南林业大学 西南山区森林资源保护与利用教育部重点实验室，云南 昆明 650224）
Author(s):
ZHANG Qian1; REN Hailin1; WANG Jie1; NIE Lili1; WANG Haoyu1; ZHANG Hanyao1; 2
(1.Yunnan Provincial Key Laboratory for Conservation and Utilization of In-forest Resource，Southwest Forestry University，Kunming,Yunnan 650224;2.Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China Ministry of Education，Southwest Forestry University，Kunming,Yunnan 650224)
关键词:
猕猴桃; FBA基因家族; 干旱胁迫; 转录组
Keywords:
kiwifruit; FBA gene family; drought stress; transcriptome
分类号:
S 663.4
DOI:
10.11937/bfyy.20251039
文献标志码:
A
摘要:
以猕猴桃为试材，采用TBtools、HMMER等软件对猕猴桃基因家族成员进行生物信息学分析，研究了猕猴桃的FBA基因在干旱胁迫下的响应，以期为培育耐旱猕猴桃新品种提供参考依据。结果表明:共鉴定出9个AcFBA基因，并进而对这些基因进行了系统发育分析。该物种被划分为2个亚族，9个基因不均匀的分布在8条染色体上。基因结构分析结果表明，这些AcFBA基因内含子数量较少，暗示其基因结构相对较为保守。此外，AcFBA基因中的顺式作用元件主要可划分为两大类别：一类是与激素响应相关的顺式作用元件，另一类则是与环境压力响应相关的顺式作用元件。在干旱胁迫下，共有5个AcFBA基因出现了明显的响应，3个上调2个下调。其中AcFBA4、AcFBA6 2个FBA基因可以通过正向调控干旱胁迫，降低干旱对猕猴桃植株的伤害。富集分析表明，共有8个AcFBA基因富集到8种KEGG通路中，且每个KEGG通路中均有5个AcFBA基因是DEGs。表明AcFBA基因能够参与植物的光合和代谢过程来增强植物对干旱胁迫的耐受性。
Abstract:
Taking kiwifruit as the experimental material，bioinformatics analyses of the kiwifruit FBA gene family members were conducted using software such as TBtools and HMMER to study the response of kiwifruit FBA genes under drought stress,in order to provide reference for the breeding of drought-resistant new varieties of kiwifrunt.The results showed that a total of 9 AcFBA genes were identified，and phylogenetic analysis was performed on these genes.This species was divided into 2 subfamilies，with the 9 genes unevenly distributed across 8 chromosomes.Gene structure analysis revealed that these AcFBA genes had relatively few introns，suggesting that their gene structures were relatively conserved.In addition，the cis-acting elements in the AcFBA genes could be mainly classified into two categories,one associated with hormone responses and the other with environmental stress responses.Under drought stress，a total of 5 AcFBA genes showed significant responses，with 3 being upregulated and 2 downregulated.Among them，the AcFBA4 and AcFBA6 genes might positively regulate the response to drought stress，reducing the damage of drought to kiwi plants.Enrichment analysis indicated that 8 AcFBA genes were enriched in 8 KEGG pathways，and in each KEGG pathway，5 AcFBA genes were regarded as differentially expressed genes (DEGs).This demonstrated that AcFBA genes can participate in photosynthesis and metabolic processes to enhance plant tolerance to drought stress.

参考文献/References:

［1］NGOENNET S,HONDA M,PATIPONG T,et al.The effects of salts and osmoprotectants on enzyme activities of fructose-1,6-biphosphate aldolases in a halotolerant Cyanobacterium,Halothece sp.PCC 7418［J］.Life,2020,10(3):23.［2］钟彩虹,黄文俊,李大卫,等.世界猕猴桃产业发展及鲜果贸易动态分析［J］.中国果树,2021(7):101-108.［3］李新伟,李建强,SOEJARTO D D.中国猕猴桃科新异名［J］.植物分类学报,2007,45(5):633-660.［4］朱红红,王悦欣,武雅铭,等.秦岭北麓猕猴桃冬季修剪的适宜期研究［J］.现代园艺,2025(9):13-16,21.［5］WOLBER F M,BECK K L,CONLON C A,et al.Kiwifruit and mineral nutrition［J］.Advances in Food and Nutrition Research,2013,68:233-256.［6］罗犇,王文欣,王凤玲,等.静磁场辅助超冰温对鲜切猕猴桃贮藏品质的影响［J］.保鲜与加工,2025,25(2):1-8.［7］AVILA G A,CHEN J,LI W,et al.Seasonal abundance and diversity of egg parasitoids of Halyomorpha halys in kiwifruit orchards in China［J］.Insects,2021,12(5):428.［8］ZHAO W,LIU H,ZHANG L,et al.Genome-wide identification and characterization of FBA gene family in polyploid crop Brassica napus［J］.International Journal of Molecular Sciences,2019,20(22):5749.［9］LAMESCH P,BERARDINI T Z,LI D,et al.The Arabidopsis information resource (TAIR):Improved gene annotation and new tools［J］.Nucleic Acids Research,2012,40(Database issue):D1202-D1210.［10］SAYERS E W,BOLTON E E,RODNEY B J R,et al.Database resources of the national center for biotechnology information［J］.Nucleic Acids Research,2022,50(D1):D20-D26.［11］CHEN C,CHEN H,ZHANG Y,et al.TBtools:An integrative toolkit developed for interactive analyses of big biological data［J］.Molecular Plant,2020,13(8):1194-1202.［12］RICHARDSON D P,ANSELL J,DRUMMOND L N.The nutritional and health attributes of kiwifruit:A review［J］.European Journal of Nutrition,2018,57(8):2659-2676.［13］甘思琪.猕猴桃营养品质及其综合利用研究进展［J］.食品安全导刊,2024(8):163-166.［14］LPEZ-SOBALER A M,APARICIO VIZUETE A,ORTEGA ANTA R M.Nutritional and health benefits associted with kiwifruit consumption［J］.Nutricion Hospitalaria,2016,33(Suppl 4):340.［15］STUART M E,GOODDY D C,BLOOMFIELD J P,et al.A review of the impact of climate change on future nitrate concentrations in groundwater of the UK［J］.Science of The Total Environment,2011,409(15):2859-2873.［16］高圆圆,雍清青,左杰,等.低温胁迫下不同倍性滇山茶植物激素含量变化及其相关基因的表达分析［J］.植物生理学报,2025,61(2):201-213.［17］朱宁静,何鹏亮,罗子璇,等.不同植物生长调节剂对干旱胁迫下油茶产量的影响［J］.现代农业科技,2024(24):77-82.［18］ZHAO S,ZHANG Q,LIU M,et al.Regulation of plant responses to salt stress［J］.International Journal of Molecular Sciences,2021,22(9):4609.［19］ZHANG J,LIU Y,ZHOU Z,et al.Genome-wide characterization of fructose 1,6-bisphosphate aldolase genes and expression profile reveals their regulatory role in abiotic stress in cucumber［J］.International Journal of Molecular Sciences,2024,25(14):7687.［20］SHAO P,PENG Y,WU Y,et al.Genome-wide association study and transcriptome analysis reveal key genes controlling fruit branch angle in cotton［J］.Frontiers in Plant Science,2022（13）:988647.［21］赵莹.烟草果糖-1,6-二磷酸醛缩酶基因的系统鉴定、特性分析及其非生物胁迫响应研究［D］.泰安:山东农业大学,2020.［22］LI T,HOU X,SUN Z,et al.Characterization of FBA genes in potato (Solanum tuberosum L.) and expression patterns in response to light spectrum and abiotic stress［J］.Frontiers in Genetics,2024（15）:1364944.［23］LU W,TANG X,HUO Y,et al.Identification and characterization of fructose 1,6-bisphosphate aldolase genes in Arabidopsis reveal a gene family with diverse responses to abiotic stresses［J］.Gene,2012,503(1):65-74.［24］MICHELIS R,GEPSTEIN S.Identification and characterization of a heat-induced isoform of aldolase in oat chloroplast［J］.Plant Molecular Biology,2000,44(4):487-498.［25］LV G Y,GUO X G,XIE L P,et al.Molecular characterization,gene evolution,and expression analysis of the fructose-1,6-bisphosphate aldolase (FBA) gene family in wheat (Triticum aestivum L.)［J］.Frontiers in Plant Science,2017（8）:1030.［26］刘晗琪,陈兴浩,张新建,等.杨树FBA基因家族的鉴定及生物信息学分析［J］.分子植物育种,2020,18(18):5982-5990.［27］KURODA H,YANAGAWA Y,TAKAHASHI N,et al.A comprehensive analysis of interaction and localization of Arabidopsis SKP1-like (ASK) and F-box (FBX) proteins［J］.PLoS One,2012,7(11):e50009.［28］XIE Z,JIN L,SUN Y,et al.OsNAC120 balances plant growth and drought tolerance by integrating GA and ABA signaling in rice［J］.Plant Communications,2024,5(3):100782.［29］RADY M M,BORIEK S H K,ABD EL-MAGEED T A,et al.Exogenous gibberellic acid or dilute bee honey boosts drought stress tolerance in Vicia faba by rebalancing osmoprotectants,antioxidants,nutrients,and phytohormones［J］.Plants,2021,10(4):748.［30］HAN X,SHAO S,HAN X,et al.Preparation and characterization of methyl jasmonate microcapsules and their preserving effects on postharvest potato Tuber［J］.Molecules,2022,27(15):4728.［31］MAHMOOD T,KHALID S,ABDULLAH M,et al.Insights into drought stress signaling in plants and the molecular genetic basis of cotton drought tolerance［J］.Cells,2019,9(1):105.［32］VICENTE-SERRANO S M,PEA-ANGULO D,BEGUERA S,et al.Global drought trends and future projections［J］.Philosophical Transactions.Series A,Mathematical,Physical,and Engineering Sciences,2022,380(2238):20210285.［33］黄蓉,潘艳花,汤玲,等.芍药响应干旱胁迫的调控机制研究进展［J］.北方园艺,2024(23):120-125..［34］HURA T,HURA K,OSTROWSKA A.Drought-stress induced physiological and molecular changes in plants 2.0［J］.International Journal of Molecular Sciences,2023,24(2):1773.

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备注/Memo

第一作者简介：张倩（1998-），女，硕士研究生，研究方向为林木遗传育种。E-mail：871970420@qq.com.责任作者：张汉尧（1975-），男，博士，教授，博士生导师，现主要从事林木及酵母生物技术的科研与教学等工作。E-mail：zhanghanyao@swfu.edu.cn.基金项目：云南省院士（专家）工作站资助项目（202305AF150020）；云南省农业联合重点资助项目（202301BD070001-003）；乡村振兴科技项目-乡村振兴产业关键技术融合示范资助项目（202304BP090005）。收稿日期：2025-03-20

更新日期/Last Update: 2025-12-17