HOU Yinjie,HUANG Xin,FENG Mengjiao,et al.Identification of Salt Tolerance of 12 Watermelon Germplasm Resources[J].Northern Horticulture,2025,(7):1-8.[doi:10.11937/bfyy.20243868]
十二份西瓜种质资源耐盐性鉴定
- Title:
- Identification of Salt Tolerance of 12 Watermelon Germplasm Resources
- 文章编号:
- 1001-0009(2025)07-0001-08
- 分类号:
- S 651
- 文献标志码:
- A
- 摘要:
- 以12个西瓜种质资源为试材，采用盆栽300 mmol·L-1NaCl灌根进行胁迫处理的方法，研究盐胁迫9 d后对西瓜幼苗生理指标的影响，以期为筛选耐盐性较强的西瓜种质提供参考依据。结果表明：盐胁迫后西瓜幼苗的丙二醛含量（MDA）、脯氨酸含量（Pro）、超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性普遍增加；不同材料对盐胁迫的耐受能力存在差异，发生盐害的程度各不相同。通过盐害指数结合隶属函数法综合评价西瓜苗期的耐盐性，将12份西瓜种质划分为3类，3份高耐盐种质（‘W005’‘W046’和‘W004’），2份敏感种质（‘W015’和‘W023’）和7份中耐盐种质。
- Abstract:
- Twelve watermelon germplasm resources were used as test materials，and the method of stress treatment by 300 mmol·L-1 NaCl potted irrigation was used to study the effects of salt stress on physiological indexes of watermelon seedlings after 9 days，in order to provide reference for screening watermelon germplasm with strong salt tolerance.The results showed that,malondialdehyde (MDA)，proline (Pro)，superoxide dismutase (SOD)，peroxidase (POD) and catalase (CAT) activities of watermelon seedlings were generally increased after salt stress，and there were differences in salt tolerance and the degree of salt damage in different materials.The salt tolerance of watermelon at the seedling stage was comprehensively evaluated by the salt damage index combined with the subordinate function method，and the 12 watermelon germplasm were classified into three categories,three highly salt-tolerant germplasm (‘W005’ ‘W046’ and ‘W004’)，two sensitive germplasm (‘W015’ and ‘W023’) and seven medium salt-tolerant germplasm.
参考文献/References:
［1］胡远艺,谭炎宁,刘小林,等.中国耐盐碱水稻产业化发展面临的问题与建议［J］.杂交水稻,2023，38(5):1-5.［2］LU C,QIU N,LU Q,et al.Does salt stress lead to increased susceptibility of photosystem Ⅱ to photoinhibition and changes in photosynthetic pigment composition in halophyte Suaeda salsa grown outdoors?〖KG-*4〗［J］.Plant Science,2002,163(5):1063-1068.［3］ARIF Y,SINGH P,SIDDIQUI H,et al.Salinity induced physiological and biochemical changes in plants:An omic approach towards salt stress tolerance［J］.Plant Physiology and Biochemistry,2020,156:64-77.［4］BINTE M M,AKTER IVY N,MOYNUL HAQUE M,et al.Oxidative stress tolerance mechanism in rice under salinity［J］.Phyton,2020,89(3):497-517.［5］FLOWERS T J,MUNNS R,COLMER T D.Sodium chloride toxicity and the cellular basis of salt tolerance in halophytes［J］.Annals of Botany,2015,115(3):419-431.［6］黎天,李继强,冯玉喜.人工模拟盐、碱胁迫对油菜种子萌发的影响［J］.中国农业信息,2011(10):32-33.［7］王喜涛.黄瓜种质资源耐盐性评价及其遗传分析［D］.哈尔滨:东北农业大学,2014.［8］李世玉.26份甜瓜材料耐盐性评价及外源NO对甜瓜盐胁迫的缓解效应［D］.杨凌:西北农林科技大学,2022.［9］ZHANG H,GONGG,GUO S,et al.Screening the USDA watermelon germplasm collection for drought tolerance at the seedling stage［J］.HortScience,2011,46(9):1245-1248.［10］ABDEL H A L,HE C.Arbuscular mycorrhizal influence on growth,photosynthetic pigments,osmotic adjustment and oxidative stress in tomato plants subjected to low temperature stress［J］.Acta Physiologiae Plantarum,2011,33(4):1217-1225.［11］张秋芳.盐胁迫对盐生植物叶片SOD及光合特性的效应［D］.济南:山东师范大学,2002.［12］韩志平,郭世荣,李娟.设施栽培西瓜品种对盐胁迫的响应［J］.江苏农业学报,2008,24(6):888-895.［13］周广,孙宝腾,张乐华,等.井冈山杜鹃叶片抗氧化系统对高温胁迫的响应［J］.西北植物学报,2010,30(6):1149-1156.［14］张涛,马肖静,朱新红,等.NaCl胁迫对不同耐盐性辣椒幼苗生理生化指标的影响［J］.山东农业科学,2021,53(12):38-43.［15］秦红艳,艾军,李昌禹,等.山葡萄组培苗盐害指数和耐盐指数主成分分析［J］.北方园艺,2013(16):18-21.［16］张清航,张永涛.植物体内丙二醛(MDA)含量对干旱的响应［J］.林业勘查设计,2019,48(1):110-112.［17］杨建军,张国斌,郁继华,等.盐胁迫下内源NO对黄瓜幼苗活性氧代谢和光合特性的影响［J］.中国农业科学,2017,50(19):3778-3788.［18］KHAMPHENG B,沈镭,钟帅,等.脯氨酸引发提高烟草种子和幼苗抗逆性及其与抗氧化系统的关系［J］.山西农业科学,2019,47(1):39-48.［19］徐亚军,赵龙飞,邢鸿福,等.内生细菌对盐胁迫下小麦幼苗脯氨酸和丙二醛的影响［J］.生态学报,2020,40(11):3726-3737.［20］江毅,李晨,刘凡凡,等.盐胁迫下不同野生草莓品种生理响应及耐盐性差异分析［J］.北方园艺,2024(18):32-40.［21］LIU X，SHI J，ZHANG X Y，et al.Screening salt tolerance germplasms and tagging the tolerance gene(s) using microsatellite (SSR) markers in wheat［J］.Acta Botanica Boreali-Occidentalia Sinica，2001,43(9):948-954.
相似文献/References:
[1]林燚,王驰,杨瑜斌,等.西瓜嫁接栽培植株凋萎原因及对策[J].北方园艺,2013,37(04):59.
[2]陈 潇,盛萍萍,刘润进,等.西瓜与辣椒间作体系接种AM真菌对连作西瓜植株[J].北方园艺,2014,38(12):6.
CHEN Xiao,SHENG Ping-ping,LIU Run-jin,et al.Effect of Intercropping System of Pepper-Watermelon Crops Inoculating Arbuscular Mycorrhizal Fungus on Growth and Defence Enzymes Activity of the Continuous Cropping Watermelon[J].Northern Horticulture,2014,38(7):6.
[3]李 勤,皇甫自起,陈大伟.豫东地区地膜覆盖栽培西瓜品种比较试验[J].北方园艺,2014,38(12):23.
LI Qin,HUANGFU Zi-qi,CHEN Da-wei.Comparison Test on Film Mulching Cultivating Watermelon Varieties in the East of Henan Province[J].Northern Horticulture,2014,38(7):23.
[4]冀媛媛,杨静慧,李金龙,等.六种植物耐盐性的离体快速分析[J].北方园艺,2014,38(12):53.
JI Yuan-yuan,YANG Jing-hui,LI Jin-long,et al.Study on Plant Salt Tolerance of Six Species by a Rapid Method in vitro[J].Northern Horticulture,2014,38(7):53.
[5]杨 飞,郭海波,吴 菊,等.NaCl胁迫对白菜种子萌发及幼苗生长的影响[J].北方园艺,2014,38(01):26.
YANG Fei,GUO Hai-bo,WU Ju,et al.Effect of NaCl Stress on the Seed Germination and Seedling Growth of Chinese Cabbage Cultivars[J].Northern Horticulture,2014,38(7):26.
[6]郭松,刘声峰,于 蓉,等.压砂地西瓜水肥耦合模型及优化组合方案[J].北方园艺,2013,37(17):8.
GUO Song,LIU Sheng-feng,YU Rong,et al.Optimized Combination Scheme and Water-Fertilizer Coupling Model on Watermelon in Sand Field[J].Northern Horticulture,2013,37(7):8.
[7]徐雪莲,张银东,符悦冠,等.蚜虫侵害对西瓜保护酶活性的影响[J].北方园艺,2014,38(01):109.
XU Xue-lian,ZHANG Yin-dong,FU Yue-guan,et al.Effect of Aphid Invasion on Protective Enzyme Activities of Different Watermelon Varieties[J].Northern Horticulture,2014,38(7):109.
[8]王林闯,汪翠芳,罗德旭,等.木薯渣复合基质对西瓜幼苗生长的影响[J].北方园艺,2014,38(04):5.
WANG Lin-chuang,WANG Cui-fang,LUO De-xu,et al.Effect of Compound Substrate With Cassava Dregs on the Growth of Watermelon Seedling[J].Northern Horticulture,2014,38(7):5.
[9]韩亚楠,毕美光,刘润进,等.AM真菌对连作西瓜生长及其枯萎病的影响[J].北方园艺,2013,37(13):150.
HAN Ya-nan,BI Mei-guang,LIU Run-jin,et al.Effects of Arbuscular Mycorrhizal Fungus on Growth and Fusarium Wilt of Watermelon Seedlings in Continuous Cropping Soils[J].Northern Horticulture,2013,37(7):150.
[10]江超,夏阳,杨克强.盐胁迫下紫花苜蓿苗期根部生长及生理特性研究[J].北方园艺,2014,38(05):68.
JIANG Chao,XIA Yang,YANG Ke-qiang.Research on the Root Physiological Characteristics of Medicago sativa L.Seedling Under Salt Stress[J].Northern Horticulture,2014,38(7):68.
备注/Memo
第一作者简介：侯尹婕(1999-)，女，硕士研究生，研究方向为西瓜种质资源调查与抗性鉴定。E-mail：2058376912@qq.com.责任作者：魏春华(1986-)，男，博士，副教授，现主要从事蔬菜育种与生物技术等研究工作。E-mail：xjwend020405@nwafu.edu.cn.基金项目：宁夏农林科学院高质量发展和生态保护科技创新示范资助项目（NGSB-2021-7）；国家现代农业产业技术体系资助项目（CARS-25）。收稿日期：2024-09-27