LIANG Huan,LIU Jiangfeng,ZHU Juhong,et al.Effects of Exogenous Auxin and Cytokinin Application on the Healing Process of Oriental Melon Grafted Onto Pumpkin Under Low Night Temperature[J].Northern Horticulture,2025,(2):38-43.[doi:10.11937/bfyy.20242647]
外源物质处理对低夜温条件下薄皮甜瓜嫁接愈合进程的影响
- Title:
- Effects of Exogenous Auxin and Cytokinin Application on the Healing Process of Oriental Melon Grafted Onto Pumpkin Under Low Night Temperature
- 文章编号:
- 1001-0009(2025)02-0038-06
- Keywords:
- oriental melon; grafting healing; auxin; cytokinin; low night temperature
- 分类号:
- S 652
- 文献标志码:
- A
- 摘要:
- 以薄皮甜瓜‘美浓’为接穗，南瓜品种‘得力2号’为砧木，在28 ℃/18 ℃的温度条件下，设置20个不同浓度和类型的生长素和细胞分裂素处理，观测嫁接愈合过程中木质部和韧皮部的连通情况，探究外源物质喷施对薄皮甜瓜嫁接愈合进程的影响，以期为甜瓜高效嫁接愈合提供参考依据。结果表明：喷施0.5 mg·L-1 IAA或10 mg·L-1 KT均能促进甜瓜嫁接木质部连通，相比于CK，在嫁接后5 d时，喷施0.5 mg·L-1 IAA和10 mg·L-1 KT处理的甜瓜嫁接苗的木质部连通率分别增长了236.00%和89.00%。0.5 mg·L-1 IAA与10 mg·L-1 KT按照1∶1比例复配后喷施，对甜瓜嫁接愈合韧皮部和木质部连通均有显著促进效果，在嫁接5 d后，甜瓜嫁接苗的韧皮部连通率和木质部连通率分别增长了42.99%和246.50%。综上所述，低夜温条件下，找到合适的激素和浓度能够加快愈合进程。
- Abstract:
- Taking the thin skinned sweet melon ‘Meinong’ as the scion and the pumpkin variety ‘Deli No.2’ as the rootstock,20 different concentrations and types of auxin and cytokinin treatments were set at a temperature of 28 ℃/18 ℃ to observe the connection between the xylem and phloem during the grafting healing process.The effect of exogenous substance spraying on the grafting healing process of thin skinned sweet melon was explored,in order to provide reference for the efficient grafting and healing of melon.The results showed that spraying 0.5 mg·L-1 IAA or 10 mg·L-1 KT could promote the xylem connectivity.Compared with CK，at 5 days after grafting,the xylem connectivity of melon grafted seedlings treated with 0.5 mg·L-1 IAA and 10 mg·L-1 KT increased by 236.00% and 89.00%，respectively.Furthermore，0.5 mg·L-1 IAA and 10 mg·L-1 KT mixed with five ratio.The optimum hormone combination ratio was 1∶1，which significantly promoted the phloem and xylem connectivity of melon grafting.At 5 days after grafting，the phloem and xylem connectivity rates of melon grafted seedlings increased by 42.99% and 246.50%，respectively.In conclusion，the appropriate hormone and concentration can effectively accelerate the healing process.
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备注/Memo
第一作者简介：梁欢（1990-），女，硕士，农艺师，现主要从事蔬菜集约化育苗技术等研究工作。Email:lianghuan@wuhanagri.com.责任作者：周谟兵（1968-），男，本科，推广研究员，现主要从事蔬菜集约化育苗技术等研究工作。Email:1429773192@qq.com.基金项目：湖北省重点研发计划资助项目（2023BBB033）；武汉市知识创新专项曙光资助项目（2022020801020418）。收稿日期：2024-06-24