[1]门岩,杨忠仁,黄修梅,等.基于SSR和SRAP标记的沙芥属种质资源遗传多样性分析[J].北方园艺,2025,(2):1-7.[doi:10.11937/bfyy.20242066]
　MEN Yan,YANG Zhongren,HUANG Xiumei,et al.Genetic Diversity Analysis of Pugionium Gaertn. Resources Based on SSR and SRAP Markers[J].Northern Horticulture,2025,(2):1-7.[doi:10.11937/bfyy.20242066]
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基于SSR和SRAP标记的沙芥属种质资源遗传多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2025年2 页码: 1-7 栏目: 出版日期: 2025-01-31
Title:
Genetic Diversity Analysis of Pugionium Gaertn. Resources Based on SSR and SRAP Markers
文章编号:
1001-0009(2025)02-0001-07
作者:
门岩1; 杨忠仁1; 黄修梅1; 张晓辉2; 张凤兰1; 郝丽珍1
（1.内蒙古农业大学 园艺与植物保护学院，内蒙古 呼和浩特 010000；2.中国农业科学院 蔬菜花卉研究所，农业农村部园艺作物生物学与种质创制重点实验室，北京 100081）
Author(s):
MEN Yan1; YANG Zhongren1; HUANG Xiumei1; ZHANG Xiaohui2; ZHANG Fenglan1; HAO Lizhen1
(1.College of Horticulture and Plant Protection，Inner Mongolia Agricultural University，Hohhot，Inner Mongolia 010000；2.Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Beijing 100081)
关键词:
沙芥属; 遗传多样性; 亲缘关系; SSR标记; SRAP标记
Keywords:
Pugionium Gaertn.; genetic diversity; phylogenetic; SSR marker; SRAP marker
分类号:
S 949.748.3
DOI:
10.11937/bfyy.20242066
文献标志码:
A
摘要:
以沙芥属植物和十字花科部分属植物叶片为试材，采用SSR和SRAP 2种分子标记技术，研究了沙芥属植物的亲缘关系与遗传多样性，以期为沙芥属植物应用提供参考依据。结果表明：7对引物检测到的有效等位基因数平均值、多态性位点百分比平均值、Nei′s基因多样性平均值和Shannon多态性指数平均值分别为1.260 7、54.88%、0.159 3和0.264 4；沙芥属植物遗传分化75.18%发生在沙芥种内和斧翅沙芥种各类型内，24.82%存在于种间和斧翅沙芥种内各个类型间；SRAP扩增结果显示斧翅沙芥种内不同类型之间的有效等位基因数平均值、多态性位点比率平均值、Nei′s基因多样性平均值、Shannon多态性指数的平均值分别为1.703 2、70.32%、0.234 2、0.353 3；斧翅沙芥种遗传分化80.18%存在于类型内，19.82%存在于不同类型间。SSR分析表明沙芥种的遗传多样性水平要低于斧翅沙芥种，斧翅沙芥种内特殊类型的遗传多样性水平最高，依次是宽翅类型、距果类型、齿冠类型和斧形类型。SRAP对沙芥属与十字花科部分属植物亲缘关系分析表明沙芥属与供试的芸薹属、萝卜属、大青属、诸葛菜属、拟南芥属的亲缘关系均较远。
Abstract:
Taking the leaves of Pugionium Gaertn. plants and parts of brassicaceae plants as experimental materials,the genetic diversity and phylogenetic relationships of Pugionium Gaertn. were studied by using SSR and SRAP molecular marker techniques，in order to provide reference for the application Pugionium Gaertn..The results showed that the average number of effective alleles,the average percentage of polymorphic loci,the average values of Nei′s and Shannon′s detected by 7 pairs of primers were 1.260 7,54.88%,0.159 3 and 0.264 4，respectively；75.18% of genetic differentiation occurs within Pugionium cornutum (L.) Gaertn.and within various types of Pugionium dolabratum Maxim，while 24.82% occurs between species and within various types of Pugionium dolabratum Maxim.The SRAP amplification results showed that the average number of effective alleles,the average percentage of polymorphic loci,the average values of Nei′s and Shannon′s were 1.703 2，70.32%，0.234 2 and 0.353 3，respectively；80.18% of the genetic differentiation occurs within the types and 19.82% was between types.SSR analysis showed that the genetic diversity level of the Pugionium cornutum (L.) Gaertn.was lower than that of the Pugionium dolabratum Maxim.The genetic diversity level of the special types was the highest，followed by the broadwing type，calcanthus type，crown type and axe type.SRAP analysis of the phylogenetic relationship between the Pugionium Gaertn.plants and parts of the Brassicaceae family showed that the Pugionium Gaertn.had a relatively distant phylogenetic relationship with the tested Brassica,Raphanus,Clerodendrum,Orychophragmus and Arabidopsis.

参考文献/References:

［1］宁可真,袁学松,杨忠仁,等.邻苯二甲酸和肉桂酸对沙芥属植物生长及保护酶活性的影响［J］.西北农林科技大学学报(自然科学版),2021,49(11):97-104.［2］张韶华,张晓辉,杨忠仁,等.高温胁迫对沙芥与斧翅沙芥幼苗生长和叶绿素荧光动力参数的影响［J］.北方园艺,2024(10):1-8.［3］张凤兰.沙芥属植物遗传多样性及其亲缘关系研究［D］.呼和浩特:内蒙古农业大学,2009.［4］胡慧.中国苎麻属无融合生殖种质的遗传多样性研究［D］.北京:中国农业科学院,2013.［5］程远毅.秦岭南麓野生草莓属植物分类及形态学遗传多样性研究［D］.长春:吉林农业大学,2020.［6］王晓英,王长宪,华芳霞.春兰的遗传多样性研究进展［J］.山东林业科技,2013,43(3):96-99.［7］周嘉康.基于叶片形态、理化性质和SSR分子标记的构树类群分析［D］.长沙:中南林业科技大学，2022.［8］顾晓燕,郭志慧,张新全,等.老芒麦种质资源遗传多样性的SRAP分析［J］.草业学报,2014,23(1):205-216.［9］赵一之.沙芥属的分类校正及其区系分析［J］.内蒙古大学学报(自然科学版),1999,30(2):197-199.［10］杜永光，郝丽珍，王萍，等.沙芥生长发育及种子产量与播种期关系研究.华北农学报，2006，21(5):118-122.［11］唐志红,王倩,何芳兰,等.沙芥属(十字花科)的起源、分类与进化研究进展［J］.西北植物学报,2014,34(8):1714-1720.［12］刘林娅.辣椒种质资源遗传多样性的形态学及ISSR标记分析［D］.海口:海南大学,2013.［13］孙阳阳,王晓英,董丽娟,等.基于SRAP分子标记红仁核桃自然杂交F1代的遗传多样性分析［J］.北方园艺,2023(20):28-33.［14］李宁,王飞,尹延旭,等.辣椒种质遗传多样性分析［J］.辣椒杂志,2017,15(4):1-8,13.［15］王丹丹.基于SRAP和TRAP标记的新疆10种野生葱蒜的遗传多样性［D］.乌鲁木齐:新疆农业大学,2018.［16］蔡一鸣,蒋双丰,毛光志,等.基于SSR分子标记的41份茶树种质资源遗传多样性及亲缘关系分析［J］.中国农学通报,2023,39(30):54-60.［17］MA S,KHAYATNEZHAD M,MINAEIFAR A A.Genetic diversity and relationships among Hypericum L.species by ISSR markers:A high value medicinal plant from Northern of Iran［J］.Caryologia,2021,74(1):97-107.［18］白晓倩,陈于,张仕杰,等.基于表型性状和SSR标记的板栗品种遗传多样性分析及分子身份证构建［J］.植物遗传资源学报,2022,23(4):972-984.［19］贾艳艳,邱玉鹏,周欣莹,等.基于形态标记和ISSR分子标记的长瓣铁线莲遗传多样性分析［J］.草地学报,2024,32(1):75-86.［20］ISMAIL N A,RAFII M Y,MAHMUD T M M,et al.Genetic diversity of torch ginger (Etlingera elatior) germplasm revealed by ISSR and SSR markers［J］.BioMed Research International,2019,2019:5904804.［21］骆亮,张文春,李龙,等.不同居群朱砂根(Ardisia crenata)的荧光ISSR遗传多样性分析［J］.分子植物育种,2021,19(18):6235-6247.［22］李东宾,徐婧,何立平,等.四明山野生杜鹃花资源遗传多样性和亲缘关系分析［J］.林业科技,2023,48(1):15-19,47.［23］HUCL P,MATUS-CDIZ M.Isolation distances for minimizing out-crossing in spring wheat［J］.Crop Science,2001,41(4):1348-1351.［24］LLEWELLYN D,FITT G.Pollen dispersal from two field trials of transgenic cotton in the Namoi Valley,Australia［J］.Molecular Breeding,1996,2(2):157-166.［25］HANSON B D,MALLORY-SMITH C A,SHAFII B,et al.Pollen-mediated gene flow from blue aleurone wheat to other wheat cultivars［J］.Crop Science,2005,45(4):1610-1617.［26］MATUS-C M A，HUCL P，DUPUIS B.Pollen-mediated gene flow in wheat at the commercial scale［J］.Crop Science，2007，47：573-581.

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备注/Memo

第一作者简介：门岩(1994-)，女，博士研究生，研究方向为沙生植物。Email：1524193606@qq.com.责任作者：张凤兰(1979-)，女，博士，副教授，现主要从事沙生植物等研究工作。Email：nmndzhangfenglan@imau.edu.cn.基金项目：国家重点研发计划资助项目(2021YFD1200201)；国家自然科学基金资助项目(31760570)；内蒙古自治区科技成果转专项资金资助项目(2021CG0023)；内蒙古自治区科技计划资助项目(2021GG0084)。收稿日期：2024-05-16

更新日期/Last Update: 2025-02-08