ZHANG Songlin,WANG Xiaoyu,GUO Junxia,et al.Effects of Microbial Fertilizer on Growth,Quality and Yield of Chuandanshen Under Continuous Cropping Obstacles[J].Northern Horticulture,2024,(16):102-108.[doi:10.11937/bfyy.20240493]
微生物菌肥对连作障碍下川丹参生长、品质和产量的影响
- Title:
- Effects of Microbial Fertilizer on Growth,Quality and Yield of Chuandanshen Under Continuous Cropping Obstacles
- 文章编号:
- 1001-0009(2024)16-0102-07
- 分类号:
- S 567.9
- 文献标志码:
- A
- 摘要:
- 以川丹参为试材,采用大田试验,研究了微生物菌肥对连作1年和连作2年的川丹参生长、品质和产量的影响,以期为提高川丹参产量和品质提供参考依据。结果表明:施用微生物菌肥后,连作1年和连作2年川丹参的生物量高于对照,其中地上部鲜质量、根鲜质量、地上部干质量、根干质量最多分别增加了58.3%、78.1%、77.1%、118.5%;与此同时,抗氧化酶活性和丙二醛(MDA)含量低于对照,可溶性糖和可溶性蛋白质高于对照,其中超氧化物酶(SOD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)和MDA最多分别比对照低53.5%、26.0%、76.5%、66.8%,可溶性糖和可溶性蛋白质分别比对照高27.8%、47.8%;此外,丹参酮ⅡA含量最多比对照高117.1%,丹酚酸B含量变化不明显。相关性分析表明,丹参酮ⅡA含量和地上部干质量、根干质量呈极显著正相关。
- Abstract:
- Taking Chuandanshen as the test material,a field experiment was conducted to study the effects of microbial fertilizer on its growth,quality,and yield in one year and two years of continuous cropping obstacles,in order to provide reference for the improvement of Chuandanshen′s yield and quality.The results showed that the biomass of Chuandanshen in one and two years of continuous cropping obstacle was higher than CK after the application of microbial fertilizer,in which the shoot fresh weight,the root fresh weight,the shoot dry weight,and root dry weight increased by 58.3%,78.1%,77.1% and 118.5% at most,respectively.At the same time,the activities of antioxidant enzymes and MDA content were lower than CK,and the content of soluble sugars and soluble proteins were higher than CK,in which the activities of SOD,CAT,PPO and MDA content were at most 53.5%,26.0%,76.5% and 66.8% lower than CK,and soluble sugars and soluble proteins were 27.8% and 47.8% higher than CK,respectively;in addition,the content of tanshinone ⅡA was at most 117.1% higher than CK,but the change of tanshinolic acid B content was not obvious.Correlation analysis showed that the content of tanshinone ⅡA was extremely significantly positively correlated with shoot dry weight and root dry weight.
参考文献/References:
[1]SU C Y,MING Q L,RAHMAN K,et al.Salvia miltiorrhiza:Traditional medicinal uses,chemistry,and pharmacology[J].Chinese Journal of Natural Medicines,2015,13(3):163-182.[2]翟俊杰,左亚锋,王孟虎,等.丹参品种、产地及栽培历史的本草考证[J].辽宁中医药大学学报,2024,26(5):130-137.[3]蒲晓芳,李佳.丹参产况和商品丹参的主要流向调查[J].中国医药指南,2013,11(15):666-667.[4]王晓宇,张松林,罗冰,等.基于商品规格等级评价的丹参古今文献论述[J].时珍国医国药,2020,6(8):1967-1969.[5]黄菊英,崔东,刘玉珊,等.丹参根际微生物和连作障碍的研究进展[J].江苏农业科学,2023,51(3):8-15.[6]张辰露,孙群,叶青.连作对丹参生长的障碍效应[J].西北植物学报,2005,25(5):1029-1034.[7]LIU H,NIU M,ZHU S,et al.Effect study of continuous monoculture on the quality of Salvia miltiorrhiza bge roots[J].BioMed Research International,2020,2020:4284385.[8]檀国印,杨志玲,袁志林,等.药用植物连作障碍及其防治途径研究进展[J].西北农林科技大学学报(自然科学版),2012,40(4):197-204.[9]张重义,林文雄.药用植物的化感自毒作用与连作障碍[J].中国生态农业学报,2009,17(1):189-196.[10]XIONG W,ZHAO Q,ZHAO J,et al.Different continuous cropping spans significantly affect microbial community membership and structure in a vanilla-grown soil as revealed by deep pyrosequencing[J].Microbial Ecology,2015,70(1):209-218.[11]GAO Z,HAN M,HU Y,et al.Effects of continuous cropping of sweet potato on the fungal community structure in rhizospheric soil[J].Frontiers in Microbiology,2019,10:2269.[12]ZENG J,LIU J,LU C,et al.Intercropping with turmeric or ginger reduce the continuous cropping obstacles that affect Pogostemon cablin (patchouli)[J].Frontiers in Microbiology,2020,11:579719.[13]郭兰萍,周良云,莫歌,等.中药生态农业:中药材GAP的未来[J].中国中药杂志,2015,40(17):3360-3366.[14]董林林,牛玮浩,王瑞,等.人参根际真菌群落多样性及组成的变化[J].中国中药杂志,2017,42(3):443-449.[15]ZHANG X H,LANG D Y,ZHANG E H,et al.Diurnal changes in photosynthesis and antioxidants of Angelica sinensis as influenced by cropping systems[J].Photosynthetica,2013,51(2):252-258.[16]李明杰,冯法节,张宝,等.多元组学背景下地黄连作障碍形成的分子机制研究进展[J].中国中药杂志,2017,42(3):413-419.[17]杭烨,罗夫来,赵致,等.不同间作模式下半夏产量及品质的综合评价[J].北方园艺,2018(13):132-140.[18]张子龙,王文全.药用植物连作障碍的形成机理及其防治[J].中国农业科技导报,2009,11(6):19-23.[19]XIONG J,PENG S,LIU Y,et al.Soil properties,rhizosphere bacterial community,and plant performance respond differently to fumigation and bioagent treatment in continuous cropping fields[J].Frontiers in Microbiology,2022,13:923405.[20]QIU M,ZHANG R,XUE C,et al.Application of bio-organic fertilizer can control Fusarium wilt of cucumber plants by regulating microbial community of rhizosphere soil[J].Biology and Fertility of Soils,2012,48(7):807-816.[21]NAHER U A,BISWAS J C,MANIRUZZAMAN M,et al.Bio-organic fertilizer:A green technology to reduce synthetic N and P fertilizer for rice production[J].Frontiers in Plant Science,2021,12:602052.[22]李俊,姜昕,马鸣超,等.我国微生物肥料产业需求与技术创新[J].中国土壤与肥料,2019(2):1-5.[23]张德林,余星语,喻文,等.3种微生物菌肥对川芎生长发育、产质量和镉富集的影响[J].中国实验方剂学杂志,2022,8(5):124-132.[24]赖锟阳,肖建才,王红阳,等.微生物菌肥调控药用植物品质形成的作用机制及应用与展望[J].中国中药杂志,2024,49(4):912-923.[25]张乔会,覃章辉,万海英,等.不同肥料处理下黄连生长指数分析[J].北方园艺,2022(8):89-94.[26]WU L,YANG B,LI M,et al.Modification of rhizosphere bacterial community structure and functional potentials to control Pseudostellaria heterophylla replant disease[J].Plant Disease,2020,104(1):25-34.[27]李娟,王文丽,赵旭.生物肥料对当归生长及土壤酶活性、微生物多样性的影响[J].广东农业科学,2020,47(6):39-46.[28]HELGASON T,FITTER A H.Natural selection and the evolutionary ecology of the arbuscular mycorrhizal fungi (Phylum Glomeromycota)[J].Journal of Experimental Botany,2009,60(9):2465-2480.[29]VERMA R K,VERMA S K,PANKAJ U,et al.Improvement in the yield and quality of kalmegh (Andrographis paniculata Nees) under the sustainable production system[J].Natural Product Research,2015,29(3):297-300.[30]XIE Z,CHU Y,ZHANG W,et al.Bacillus pumilus alleviates drought stress and increases metabolite accumulation in Glycyrrhiza uralensis Fisch[J].Environmental and Experimental Botany,2019,158:99-106.[31]吴红淼,林文雄.药用植物连作障碍研究评述和发展透视[J].中国生态农业学报(中英文),2020,28(6):775-793.[32]郭志祥,钮颜宇,徐淑慧,等.微生物肥对连作地黄生长及产量品质的影响[J].北方园艺,2022(19):100-106.[33]李小玲,周武先,蒋小刚,等.微生物菌肥对川党参连作障碍及紫纹羽病的防控效果[J].中国农业科技导报,2023,25(3):119-131.[34]李艳春,陈志鹏,林伟伟,等.茶树连作障碍形成机制及调控措施研究进展[J].生态科学,2019,38(5):225-232.[35]KIM Y S,BALARAJU K,JEON Y H.Biological characteristics of Bacillus amyloliquefaciens AK-0 and suppression of ginseng root rot caused by Cylindrocarpon destructans[J].Journal of Applied Microbiology,2017,122(1):166-179.[36]欧洪,郭冬琴,林俊杰,等.AM真菌对滇重楼根际土壤微生物数量及酶活性的影响[J].中药材,2016,39(5):948-955.[37]张大琪,任立瑞,杜洪志,等.微生物菌剂处理增加了生姜土壤中有益微生物的相对丰度[J].植物保护,2023,49(4):55-66.[38]姚阳阳,杨涛,王引权,等.两类根际复配促生菌剂对当归生长、生理特征及药效成分的影响[J].中国实验方剂学杂志,2022,28(8):131-138.
相似文献/References:
[1]杨 爽,李海鹏,杨培鑑,等.微生物菌肥对草莓光合特性的影响[J].北方园艺,2014,38(11):165.
YANG Shuang,LI Hai-peng,YANG Pei-jian,et al.Effect of Biofertilizer on Photosynthesis Characters of Strawberries[J].Northern Horticulture,2014,38(16):165.
[2]宋 家 清1,郑 秀 社 1,张 庆 国 1,等.活性微生物菌肥对滨海盐碱土改良的影响[J].北方园艺,2010,34(18):0.[doi:10.11937/bfyy.201018020]
,,et al.I m p r o v i n g E f f e c t o f M i c r o b i a l O r g a n i c F e r t i l i z e r o n C o a s t a l S a l i n e - a l k a l i S o i l[J].Northern Horticulture,2010,34(16):0.[doi:10.11937/bfyy.201018020]
[3]田雪莲,尹显慧,龙友华,等.不同肥料处理对番茄产量、品质及经济效益的影响[J].北方园艺,2015,39(23):178.[doi:10.11937/bfyy.201523049]
TIAN Xuelian,YIN Xianhui,LONG Youhua,et al.Effect of Different Fertilizer Treatments on Yield,Quality and Economic Benefit of Tomato[J].Northern Horticulture,2015,39(16):178.[doi:10.11937/bfyy.201523049]
[4]谢东锋,薛书浩,曾钰婷,等.采前微生物菌肥处理连作土壤对番茄生长及抗性酶活性的影响[J].北方园艺,2018,42(21):136.[doi:10.11937/bfyy.20174662]
XIE Dongfeng,XUE Shuhao,ZENG Yuting,et al.Effects of Preharvest Microbial Fertilizers Treatment of Continuous Cropping Soil on Tomato Growth and Resistance Enzymes Activity[J].Northern Horticulture,2018,42(16):136.[doi:10.11937/bfyy.20174662]
[5]杨志刚,叶英杰,常海文,等.微生物菌肥及土壤修复剂对干制辣椒生长、品质及产量的影响[J].北方园艺,2020,44(19):1.[doi:10.11937/bfyy.20194763]
YANG Zhigang,YE Yingjie,CHANG Haiwen,et al.Effects of Microbial Fertilizer and Soil Amendment on the Growth,Quality and Yield of Dry Pepper[J].Northern Horticulture,2020,44(16):1.[doi:10.11937/bfyy.20194763]
[6]冯敬涛,于天武,吴晓娴,等.微生物菌肥对苹果土壤理化特性及养分吸收的影响[J].北方园艺,2021,(02):97.[doi:10.11937/bfyy.20191864]
FENG Jingtao,YU Tianwu,WU Xiaoxian,et al.Effects of Microbial Fertilizer on the Physical and Chemical Properties and Nutrient Absorption of Apple Soil[J].Northern Horticulture,2021,(16):97.[doi:10.11937/bfyy.20191864]
[7]王秀娟,韩瑛祚,何志刚,等.微生物菌肥对设施番茄养分吸收与土壤氮磷累积的影响[J].北方园艺,2021,(19):100.[doi:10.11937/bfyy.20211368]
WANG Xiujuan,HAN Yingzuo,HE Zhigang,et al.Effects of Microbial Fertilizer on Nutrient Uptake of Tomato and Soil Nitrogen and Phosphorus Accumulation in Facilities[J].Northern Horticulture,2021,(16):100.[doi:10.11937/bfyy.20211368]
[8]刘赵帆.微生物菌肥替代部分化肥对设施黄瓜生长、品质及产量的影响[J].北方园艺,2022,(02):47.[doi:10.11937/bfyy.20210275]
LIU Zhaofan.Effects of Replacing Part of Chemical Fertilizer With Microbial Bacterial Fertilizer on Growth,Quality and Yield of Cucumber in Facility[J].Northern Horticulture,2022,(16):47.[doi:10.11937/bfyy.20210275]
[9]张乔会,覃章辉,万海英,等.不同肥料处理下黄连生长指数分析[J].北方园艺,2022,(08):89.[doi:10.11937/bfyy.20214047]
ZHANG Qiaohui,QIN Zhanghui,WAN Haiying,et al.Analysis of Growth Index of Coptis chinensis Franch.Under Different Fertilizer Treatments[J].Northern Horticulture,2022,(16):89.[doi:10.11937/bfyy.20214047]
[10]王爱玲,段国琪,田时敏,等.减氮配施微生物菌肥对“富士”苹果品质和光合特性的影响[J].北方园艺,2022,(18):16.[doi:10.11937/bfyy.20220676]
WANG Ailing,DUAN Guoqi,TIAN Shimin,et al.Effects of Combined Application of Microbial Fertilizer on Quality and Photosynthetic Characteristics of ‘Fuji’ Apple[J].Northern Horticulture,2022,(16):16.[doi:10.11937/bfyy.20220676]
备注/Memo
第一作者简介:张松林(1989-),女,硕士,助理研究员,现主要从事中药材栽培与养分等研究工作。E-mail:18489972925@163.com.责任作者:李青苗(1976-),女,博士,研究员,现主要从事中药材栽培与规范化生产等研究工作。E-mail:qingmiaoli@sina.com.基金项目:2023年中央财政转移支付地方资助项目(川产道地中药资源保障能力提升研究);四川省基本科研业务专项资助项目(2022JDKY0013);四川省中医药管理局科学技术研究专项资助项目(2024MS576)。收稿日期:2024-01-30