[1]蔡静,张景怡,叶润,等.双水相提取小蓟多酚工艺优化及其生物活性研究[J].北方园艺,2024,(11):88-96.[doi:10.11937/bfyy.20234489]
　CAI Jing,ZHANG Jingyi,YE Run,et al.Optimization of Aqueous Two-phase Extraction of Polyphenols From Cirsium setosum and Investigation Into Their Biological Activity[J].Northern Horticulture,2024,(11):88-96.[doi:10.11937/bfyy.20234489]
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双水相提取小蓟多酚工艺优化及其生物活性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2024年11 页码: 88-96 栏目: 出版日期: 2024-06-15
Title:
Optimization of Aqueous Two-phase Extraction of Polyphenols From Cirsium setosum and Investigation Into Their Biological Activity
文章编号:
1001-0009(2024)11-0088-09
作者:
蔡静1; 张景怡1; 叶润1; 段宝卿1; 黄雅琴1; 刘新浩2
（1.信阳农林学院 药学院，河南 信阳 464000；2.信阳农林学院 中心实验室，河南 信阳 464000）
Author(s):
CAI Jing1; ZHANG Jingyi1; YE Run1; DUAN Baoqing1; HUANG Yaqin1; LIU Xinhao2
(1.School of Pharmacy，Xinyang Agriculture and Forestry University，Xinyang，Henan 464000；2.Central Laboratory，Xinyang Agriculture and Forestry University，Xinyang，Henan 464000)
关键词:
小蓟; 多酚; 双水相提取; α-淀粉酶; α-葡萄糖苷酶; 亚硝酸盐
Keywords:
Cirsium setosum; polyphenols; aqueous two-phase extraction; α-amylase; α-glucosidase; nitrite
分类号:
R 284.2
DOI:
10.11937/bfyy.20234489
文献标志码:
A
摘要:
以信阳小蓟为试材，采用乙醇-(NH4)2SO4双水相法（ATPE）提取多酚，研究了多酚的提取工艺及其对淀粉消化酶的抑制作用和对亚硝酸盐的清除能力，以期为小蓟多酚作为降血糖药品或食品添加剂的开发利用提供参考依据。结果表明：最佳提取工艺条件为(NH4)2SO4质量分数23%，乙醇质量分数26%，提取时间60 min，液料比30∶1 mL·g-1，提取温度81 ℃。在此条件下，多酚得率为（9.36±0.51）mg·g-1，对α-淀粉酶和α-葡萄糖苷酶抑制率的IC50值分别为0.014 mg·mL-1和0.061 mg·mL-1，明显优于阿卡波糖，且对亚硝酸盐的清除率高达82.05%，表现出良好的降血糖活性和清除亚硝酸盐的能力。
Abstract:
Aqueous two-phase extraction (ATPE) was adopted to extract polyphenols from Xinyang Cirsium setosum.The extraction process of polyphenols was studied，while also investigated its inhibitory effects on starch-digesting enzyme activity in vitro and nitrite remove ability，in order to provide reference for the development and utilization of polyphenols as hypoglycemic drugs or food additives.The results showed that the optimal extraction conditions were as follows,(NH4)2SO4 concentration was 23%，ethanol concentration was 26%，extraction time was 60 minutes，liquid-solid ratio was 30∶1 mL·g-1，extraction temperature was 81 ℃.Under these conditions,the yield of polyphenols was (9.36±0.51)mg·g-1，and the IC50 values for inhibition of α-amylase and α-glucosidase were 0.014 mg·mL-1 and 0.061 mg·mL-1，respectively，which were significantly superior to acarbose.Additionally，the nitrite clearance rate was as high as 82.05%.It showed excellent hypoglycemic activity and remarkable nitrite removal ability.

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备注/Memo

第一作者简介：蔡静(1991-)，女，硕士，讲师，现主要从事天然产物提取及纯化等研究工作。E-mail：caijing111@163.com.责任作者：黄雅琴(1986-)，女，博士，副教授，现主要从事中药材的研究与开发等研究工作。E-mail：hyaqin@yahoo.com.cn.基金项目：国家自然科学基金资助项目(12305399)；河南省科技攻关资助项目(222102110247,222102110449,242102230094,242102230129)；信阳农林学院高水平科研孵化器建设基金资助项目(FCL202105,FCL202001,FCL202009)；信阳农林学院大别山道地中药资源与系列产品开发研究科技创新团队资助项目(XNKJTD-009)。收稿日期：2023-12-23

更新日期/Last Update: 2024-06-27