FAN Huidong,XU Shilin,ZHENG Shijin,et al.Molecular Marker Detection of Resistance Genes in 105 Tomato Germplasm Resources[J].Northern Horticulture,2024,(9):24-32.[doi:10.11937/bfyy.20233930]
105份番茄种质资源的抗性基因分子标记检测
- Title:
- Molecular Marker Detection of Resistance Genes in 105 Tomato Germplasm Resources
- 文章编号:
- 1001-0009(2024)09-0024-09
- 分类号:
- S 641.2
- 文献标志码:
- A
- 摘要:
- 以105份番茄种质资源为试材，采用抗性基因分子标记辅助筛选方法，研究了105份种质资源中Mi-1、I-2、Cf-9、Ph-3、Ty-1、Ty-3、Tm-2a、Sw共8个抗性基因的分布情况，以期为开展番茄抗性基因聚合育种提供参考依据。结果表明：含Mi-1的材料28份，含I-2的材料60份，含Cf-9的材料80份，含Ph-3的材料34份，含Ty-1的材料19份，含Ty-3的材料11份，含Tm-2a的材料47份，含Sw的材料7份。105份番茄材料中，不含任何抗病基因的材料有3份；含3个以下抗性基因材料有78份，占材料总数74.3%；含4个抗病基因的材料有9份；含5个抗病基因的材料有11份；含6个抗病基因的材料有6份，分别为XIHJ-21-27、DT-27-43、722-ZT77、722-ZT79、494、495；含7个抗病基因的材料有1份，为XLJ-226-41；未检测到同时含有8个抗性基因的番茄材料。
- Abstract:
- Taking 105 tomato germplasm resources as test materials and the resistance gene molecular marker assisted screening method was used to investigate the distribution of 8 resistance genes，included Mi-1，I-2，Cf-9，Ph-3，Ty-1，Ty-3，Tm-2a and Sw，in 105 germplasm resources，in order to provide reference for the development of resistance gene polymerization breeding.The results showed that 28 materials contained Mi-1，60 materials containing I-2，80 materials containing Cf-9，34 materials containing Ph-3，19 materials containing Ty-1，11 materials containing Ty-3，47 materials containing Tm-2a，and 7 materials containing Sw.Among 105 tomato materials，3 tomato materials did not contain any resistance gene.There were 78 materials containing less than 3 resistance genes，accounting for 74.3% of the total materials.There were 9 materials containing 4 resistance genes.There were 11 materials containing 5 resistance genes.There were 6 materials containing 6 resistance genes，namely XIHJ-21-27，DT-27-43，722-ZT77，722-ZT79，494，495.One of the materials containing 7 resistance genes was XLJ-226-41.No tomato material containing 8 resistance genes at the same time was detected.
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备注/Memo
第一作者简介：范惠冬(1990-)，女，硕士，助理研究员，现主要从事蔬菜遗育种等研究工作。E-mail:fanhuidong1812@163.com.基金项目：吉林省基础科研资助项目。收稿日期：2023-11-15