GUO Lina,FANG Yumei,HAN Shiming,et al.Genetic Relationships Analysis of Six Kinds of Wild Kiwifruit Based on SSR Markers[J].Northern Horticulture,2023,(20):20-27.[doi:10.11937/bfyy.20230867]
基于SSR标记的六种野生猕猴桃亲缘关系分析
- Title:
- Genetic Relationships Analysis of Six Kinds of Wild Kiwifruit Based on SSR Markers
- 文章编号:
- 1001-0009(2023)20-0020-08
- Keywords:
- kiwifruit; transcriptome; SSR primer; genetic diversity; cluster analysis
- 分类号:
- S 663.4
- 文献标志码:
- A
- 摘要:
- 以贵州6种野生猕猴桃36个居群为试材，采用生物信息学方法，对“红阳”猕猴桃应答橘小实蝇侵害的转录组数据进行SSR位点搜索，开发EST-SSR引物后，研究了36个野生猕猴桃居群180份种质的遗传多样性与亲缘关系，以期为合理保护及开发利用野生猕猴桃资源提供参考依据。结果表明：“红阳”猕猴桃果实EST-SSR的分布频率为43.07%，6种重复类型的重复次数分布在5~24次，优势重复类型为二核苷酸，占总SSR位点的69.19%。参试的40对EST-SSR引物中有28对引物均可在6种野生猕猴桃中扩增出目的条带，在180个猕猴桃种质中共检测出了348个等位基因位点。多态性信息含量（PIC）在0.376 6（引物：P3-22）~0.930 4（引物：P4-37），平均为0.783 6，其中只有1对引物为中等多态性引物，其它均为高多态性引物。聚类分析显示，6种野生猕猴桃在遗传距离0.72处可分成2个类群，黄毛猕猴桃、毛花猕猴桃、阔叶猕猴桃被归为一类，京梨猕猴桃、异色猕猴桃、硬毛猕猴桃为一类。
- Abstract:
- Taking 36 populations of 6 wild kiwifruit species from Guizhou as experimental materials，the transcriptomic data of ‘Hongyang’ kiwifruit in response to Bactrocera dorsalis (Hendel) larval feeding was analyzed to search SSR (simple sequence repeat) loci for EST-SSR primers development through bioinformatics analysis，and genetic diversity and genetic relationships in 180 accessions of 36 populations from six kinds of wild kiwifruit were analyzed,in order to provide reference for the wild kiwifruit resources protection and utilization.The results showed that the frequency of EST-SSR was 43.07% and the repetitive number of six repeat units was 5-24.The predominant repeat motifs was dinucleotide with a proportion of 69.19%.By testing 40 pairs of EST-SSR primers，28 pairs of EST-SSR primers produced clear polymorphic fragments in all six kiwifruit species.Using the 28 EST-SSR primers that produced during preliminary screening，we detected 348 alleles in the 180 studied accessions.Polymorphism information content (PIC) ranged from a minimum of 0.376 6 (P3-22) to a maximum of 0.930 4 (P4-37) with an average of 0.783 6，which revealed only 1 pairs of SSR primers with moderate polymorphism and 27 with high polymorphism.The cluster analysis showed that the six kiwifruit species were grouped into two categories at a genetic distance of 0.72,A.fulvicoma，A.eriantha and A.latifolia were clustered as a class;A.callosa var.henryi, A.callosa var. discolor and A.chinensis var.deliciosa were anothor one.
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备注/Memo
第一作者简介：郭丽娜（1989-），女，博士，副教授，现主要从事植物学及害虫综合治理等研究工作。E-mail：15029915935@163.com.责任作者：陈志霞（1980-），女，博士，副教授，现主要从事蔬菜栽培生理与生态等研究工作。E-mail：syczx2008@163.com.基金项目：贵州省科技计划资助项目（黔科合基础［2019］1446号）；贵州省教育厅自然科学研究资助项目（黔教技［2022］054号）；六盘水师范学院高层次人才科研启动基金资助项目（LPSSYKYJJ201704）。收稿日期：2023-03-19