GUO Lina,WANG Qinqin,HAN Shiming,et al.Analysis of Morphological Variation and ITS Sequence in Six Wild Kiwifruit Species[J].Northern Horticulture,2023,(13):14-20.[doi:10.11937/bfyy.20224828]
六种野生猕猴桃形态特征变异及其ITS序列分析
- Title:
- Analysis of Morphological Variation and ITS Sequence in Six Wild Kiwifruit Species
- Keywords:
- Keywords:kiwifruit; morphological variation; cluster analysis; principal component analysis; ITS sequence analysis
- 文献标志码:
- A
- 摘要:
- 摘要：以贵州6种野生猕猴桃的26个居群为试材，采用形态特征调查与ITS序列比对分析，研究了6种野生猕猴桃的亲缘关系以及采集地点对猕猴桃形态特征变异的影响，以期为合理保护及开发利用野生猕猴桃资源提供参考依据。结果表明：野生猕猴桃6个种的形态变异主要集中在叶柄长短、果实与节间距大小和质量性状特征上，主成分分析发现最大果长、最小果长、果实毛被、茎秆毛被、叶柄毛被、叶片正面毛被、叶片背面毛被、最大叶柄长、最小叶柄长、最大节间距和最小节间距是造成种间形态特征差异的主要原因。聚类分析显示，野生猕猴桃种间与居群间的形态变异与地理分布有一定的关系，其形态特征是遗传因素和环境因子综合作用的结果。聚类分析和ITS序列分析显示，6种野生猕猴桃可分成2个类群，除硬毛猕猴桃外，其它5个种是一个单系类群
- Abstract:
- Abstract:The 26 populations from six wild kiwifruit species were used as test materials，the analysis of morphological variation and ITS sequence in 26 populations from six wild kiwifruit species were conducted to study the genetic relationships and the effect of population locality for morphology variation,in order to provide reference for future studies on the wild kiwifruit resources protection and utilization.The results showed that analysis of means showed that the six species of kiwifruit mainly differed in petiole length，internode length，fruit size and quality characteristics.The results of principal component analysis indicated that maximal fruit length，minimal fruit length，trichomes of fruit，trichomes of stem，trichomes of petiole，trichomes of leaflets (adaxial side)，trichomes of leaflets (abaxial side)，minimal petiole length，maximal petiole length，minimal internode length，maximal internode length were the main interspecific morphological differences.There were obvious morphological differentiation among species and populations which had a certain relationship with geographical distribution.The analysis of Qcluster and principal component analysis showed that the six kiwifruit species were grouped into 2 categories，A.chinensis var.deliciosa was clustered as a class and the other five kiwifruit species were anothor one.
参考文献/References:
[1]FERGUSON A R,HUANG H W.Genetic resources of kiwifruit:Domestication and breeding[J].Horticultural Reviews,2007（33）:1-121.[2]黄宏文.猕猴桃驯化改良百年启示及天然居群遗传渐渗的基因发掘[J].植物学报,2009,44(2):127-142.[3]黄宏文,钟彩虹,姜正旺,等.猕猴桃属分类资源驯化栽培[M].北京:科学出版社,2013.[4]贾照志,曾海.猕猴桃的药用价值[J].中国中医药现代远程教育,2012,10(4):118.[5]戢小梅,翟敬华,陈志伟,等.猕猴桃的营养成分与保健功能[J].湖北农业科学,2020,59(S1):386-388,420.[6]陆玲鸿,马媛媛,古咸彬,等.不同猕猴桃品种在贮藏过程中果实质构的变化[J].北方园艺,2023(7):95-101.[7]姜新,陈伯伦,张晋,等.不同果袋对低纬度高海拔地区猕猴桃果实着色及品质的影响[J].北方园艺,2022(13):35-42.[8]黄宏文,龚俊杰,王圣梅,等.猕猴桃属(Actinidia)植物的遗传多样性[J].生物多样性,2000,8(1):1-12.[9]张田.猕猴桃属植物自然居群的遗传多样性及其同域分布物种的杂交渐渗与同塑[D].武汉:中国科学院研究生院(武汉植物园),2007.[10]李作洲.猕猴桃属植物的分子系统学研究[D].武汉:中国科学院研究生院(武汉植物园),2006.[11]黄宏文,王圣梅,龚俊杰,等.猕猴桃研究进展[M].北京:科学出版社,2000.[12]黄宏文,王圣梅,姜正旺,等.猕猴桃研究进展(Ⅱ)[M].北京:科学出版社,2003.[13]黄宏文,王圣梅,姜正旺,等.猕猴桃研究进展Ⅲ[M].北京:科学出版社,2005.[14]翁周.多叶重楼种内的形态变异及其ITS序列分析[D].成都:四川大学,2007.[15]陶萌春.硃砂根(Ardisia crenata)居群的形态特征变异及聚类分析[J].植物资源与环境学报,2010,19(4):43-49.[16]杨晓慧,赵雪利,高信芬.四川木蓝复合群的形态特征变异及其ITS序列分析[J].植物科学学报,2015,33(6):727-733.[17]何子灿,钟扬,刘洪涛,等.中国猕猴桃属植物叶表皮毛微形态特征及数量分类分析[J].植物分类学报,2000,38(2):121-136,201-203.[18]高连明,杨俊波,张长芹,等.基于ITS序列分析探讨杜鹃属映山红亚属的组间关系[J].云南植物研究,2002,24(3):313-320.
相似文献/References:
[1]张 浩,周会玲,张晓晓,等.“金香”猕猴桃果实冷藏最适温度研究[J].北方园艺,2014,38(13):126.
ZHANG Hao,ZHOU Hui-ling,ZHANG Xiao-xiao,et al.The Researches of Optimum Temperature of ‘Jinxiang’ Kiwifruit During Cold Storage[J].Northern Horticulture,2014,38(13):126.
[2]金方伦,敖学熙,张发维,等.中华猕猴桃结果蔓上果实节位与生长发育动态分析[J].北方园艺,2014,38(13):15.
JIN Fang-lun,AO Xue-xi,ZHANG Fa-wei,et al.Analysis on the Growth and Development Dynamic of the Kiwi Fruit[J].Northern Horticulture,2014,38(13):15.
[3]赵俊侠,田小曼.新西兰猕猴桃管理经验对我国果业发展的启示[J].北方园艺,2014,38(11):167.
ZHAO Jun-xia,TIAN Xiao-man.The Inspiration From New Zealand Kiwi Fruit Management Experience on the Chinese Fruit Industry[J].Northern Horticulture,2014,38(13):167.
[4]金方伦,冯世华,张发维,等.不同留果量对中华猕猴桃后熟期果实品质的影响[J].北方园艺,2014,38(10):26.
JIN Fang-lun,FENG Shi-hua,ZHANG Fa-wei,et al.Effect of Different Fruit Retaining on Fruit Quality of Kiwi at Maturation Period[J].Northern Horticulture,2014,38(13):26.
[5]涂美艳,庄启国,马凤仙,等.猕猴桃溃疡病秋冬季综合防控技术[J].北方园艺,2014,38(04):112.
[6]陈永安,刘艳飞,陈 鑫,等.不同施肥措施对猕猴桃叶片营养状况及果实品质与产量的影响[J].北方园艺,2014,38(01):169.
CHEN Yong-an,LIU Yan-fei,CHEN Xin,et al.Effect of Different Fertilizing Ways on the Leaves Nutrition Status,Fruit Quality and Yield of Kiwifruit[J].Northern Horticulture,2014,38(13):169.
[7]曲雪艳,魏英,刘科鹏,等.奉新猕猴桃叶片营养及果实品质的测定与分析[J].北方园艺,2014,38(07):24.
QU Xue-yan,WEI Ying,LIU Ke-peng,et al.Measurement and Analysis on Leaves Nutrients and Fruit Quality From Kiwifruit in Fengxin County[J].Northern Horticulture,2014,38(13):24.
[8]彭家清,吴伟,肖涛,等.十堰山区猕猴桃栽培技术及发展前景[J].北方园艺,2013,37(12):40.
PENG Jia-qing,WU Wei,XIAO Tao,et al.Kiwi Fruit Cultivation Technology and Development Prospects in Shiyan Mountain[J].Northern Horticulture,2013,37(13):40.
[9]高洁,黄春辉,曲雪艳,等.微波法提取猕猴桃果实中类胡萝卜素的工艺研究[J].北方园艺,2013,37(01):11.
GAO Jie,HUANG Chun-hui,QU Xue-yan,et al.Study on Extracting Conditions of Carotenoid in Kiwifruit by Microwave[J].Northern Horticulture,2013,37(13):11.
[10]安成立,刘占德,姚春潮,等.“徐香”猕猴桃控制授粉对果实性状的影响[J].北方园艺,2013,37(07):34.
AN Cheng-li,LIU Zhan-de,YAO Chun-chao,et al.Influence of Control Pollination on Fruit Traits of ‘Xuxiang’ Kiwi[J].Northern Horticulture,2013,37(13):34.
备注/Memo
第一作者简介：郭丽娜（1989-），女，博士，副教授，现主要从事植物学及害虫综合治理等研究工作。Email：15029915935@163.com.责任作者：方玉梅（1982-），女，硕士，教授，现主要从事园艺植物栽培技术等研究工作。Email：22039392@qq.com.基金项目：贵州省科技计划资助项目（黔科合基础[2019]1446号）；贵州省教育厅自然科学研究资助项目（黔教技[2022]054号）；六盘水师范学院高层次人才科研启动基金资助项目（LPSSYKYJJ201704）。收稿日期：2023-02-08