DONG Lu,WANG Lixin.Effects of Biochar Combined With Different Bacillus on Matrix Properties and Microbial Community Composition of Tagetes erecta[J].Northern Horticulture,2023,(17):61-69.[doi:10.11937/bfyy.20224778]
生物炭与不同芽孢杆菌配施对万寿菊基质性质及微生物群落组成的影响
- Title:
- Effects of Biochar Combined With Different Bacillus on Matrix Properties and Microbial Community Composition of Tagetes erecta
- 文章编号:
- 1001-0009(2023)17-0061-09
- Keywords:
- biochar; Bacillus; Tagetes erecta; soil microorganisms; community structure; redundancy analysis
- 分类号:
- S 682.1
- 文献标志码:
- A
- 摘要:
- 以万寿菊“安提瓜”为试材，采用田间试验法，设置生物炭(BC)、生物炭+胶质芽孢杆菌(BJZ)、生物炭+侧孢芽孢杆菌(BCB)、生物炭+贝莱斯芽孢杆菌(BLS)，以不施生物炭+不施菌剂处理为对照(CK),探索了生物炭与不同芽孢杆菌菌剂配施对万寿菊土壤性质及微生物群落组成的影响，以期为芽孢杆菌和生物炭用于培育万寿菊提供参考依据。结果表明：与CK相比，相关生物炭处理(BC、BJZ、BCB、BLS)均提高了土壤pH、阳离子交换性能(CEC)、土壤养分(土壤有机质、溶解性有机碳、有效氮、有效磷、有效钾)及土壤酶活性(亚硝酸还原酶、脲酶、纤维素酶、木质素过氧化物酶)，各处理整体表现为CK＜BC＜BJZ、BCB＜BLS。高通量测序表明，BLS处理显著增加了群落多样性、促进了鞘氨醇单胞菌属(Sphingomonas)等有益菌群的增殖。冗余分析(RDA)表明，土壤脲酶是影响BLS处理微生物群落结构的重要土壤因子。综上，在万寿菊栽培过程中施用生物炭结合贝莱斯芽孢杆菌(Bacillus velezensis)可有效改善土壤性质、提高胞外酶活性及改善微生物群落结构，土壤脲酶是影响其微生物群落构建的关键土壤因子。
- Abstract:
- A field experiment was took ‘Antigua’ as the test material，the without biochar and no microbial agent was used as the control treatent(CK)，and biochar (BC)，biochar + Bacillus mucilaginosus (BJZ)，biochar + Bacillus laterosporus(BCB)，biochar + Bacillus velezensis (BLS) were set up.Which to explore the effects of biochar combined with different Bacillus agents on soil properties and microbial community composition of Tagetes erecta cultivation,in order to provide reference for Bacillus agents and biochar of Tagetes erecta planting.The results showed that compared with CK treatment，related biochar treatments (BC，BJZ，BCB，BLS) increased pH，cation exchange capacity (CEC)，soil nutrients index (OM，DOC，AN，AP，AK) and soil enzyme activities (NIR，URE，CEL，LIP)，the overall performance of each treatment was CK
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备注/Memo
第一作者简介：董璐（1988-），女，硕士，讲师，现主要从事植物栽培与园林园艺规划等研究工作。E-mail:1451766705@qq.com.基金项目：国家重点研发计划资助项目(2019YFD1001605)；河北省高等学校科学技术研究资助项目（ZD20211035）。收稿日期：2022-11-23