[1]卢颖颖,刘宏宇,黄万兵,等.基于ISSR分子标记的贵州冬荪遗传多样性分析[J].北方园艺,2023,(14):133-139.[doi:10.11937/bfyy.20224705]
　LU Yingying,LIU Hongyu,HUANG Wanbing,et al.Genetic Diversity Analysis of Phallus impudicus Strains Based on ISSR Molecular Markers[J].Northern Horticulture,2023,(14):133-139.[doi:10.11937/bfyy.20224705]
点击复制

基于ISSR分子标记的贵州冬荪遗传多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2023年14 页码: 133-139 栏目: 出版日期: 2023-07-30
Title:
Genetic Diversity Analysis of Phallus impudicus Strains Based on ISSR Molecular Markers
作者:
卢颖颖1; 2; 3; 4; 刘宏宇1; 2; 3; 4; 黄万兵1; 2; 3; 4; 桂阳1; 2; 3; 4; 朱国胜1; 2; 3; 4
（1.贵州省农业科学院 贵州省农作物品种资源研究所，贵州 贵阳 550006；2.贵州省食用菌育种重点实验室，贵州 贵阳 550006；3.贵州省食用菌现代农业产业技术体系资源育种功能实验室，贵州 贵阳 550006；4.贵州省食用菌工程技术研究中心资源育种实验室，贵州 贵阳 550006）
Author(s):
LU Yingying1; 2; 3; 4; LIU Hongyu1; 2; 3; 4; HUANG Wanbing1; 2; 3; 4; GUI Yang1; 2; 3; 4; ZHU Guosheng1; 2; 3; 4
(1.Institute of Crop Germplasm Resources，Guizhou Academy of Agricultural Sciences，Guiyang，Guizhou 550006； 2.Guizhou Key Laboratory of Edible Fungi Breeding，Guiyang，Guizhou 550006;3.Resource Breeding Function Laboratory of Guizhou Modern Agricultural Industry Technology System of Edible Fungi，Guiyang，Guizhou 550006;4.Resource Breeding Laboratory of Guizhou Engineering Research Center of Edible Fungi，Guiyang，Guizhou 550006)
关键词:
冬荪; ISSR; 遗传多样性
Keywords:
Phallus impudicus; ISSR; genetic diversity
DOI:
10.11937/bfyy.20224705
文献标志码:
A
摘要:
以不同地区的20份冬荪种质资源为试材，利用ISSR（intersimple sequence repeat 简单重复序列间扩增）分子标记技术，从78条引物中筛选出条带清晰、重复性高和特异性强的引物，在供试冬荪菌株中进行扩增。利用NTSYS 2.10e聚类分析软件UPGMA法进行聚类分析，同时利用PopGen 32软件对20份冬荪种质ISSR统计结果进行遗传多样性分析，以期通过分子标记技术评价冬荪种质资源遗传多样性，为冬荪遗传育种提供优质材料。结果表明：筛选出具备多态性ISSR引物24条，对供试冬荪菌株进行PCR扩增共获得谱带有788条，其中多态性谱带768条，多态位点比例达到975%；遗传多样性分析显示平均等位基因（Na）为1.991 3，平均有效等位基因数（Ne）为1.585 6，平均Nei′s基因多样性指数（He）为0.343 8，平均Shannon信息指数（I）为0.516 1；在遗传相似系数为0.67时， 20份冬荪菌株可分为三大类群。综上，20株冬荪种质资源间具有丰富的遗传多样性，亲缘关系较复杂；不同来源的种质由于菌种流通频繁而导致亲缘关系复杂，育种亲本的选择纯化周期较长。
Abstract:
The genetic diversity of 20 Phallus impudicus species from different regions was analyzed using Inter simple sequence repeat (ISSR) molecular markers.From 78 primers，the primers with clear bands，high repeatability and high specificity were screened and amplified in the tested strains of Phallus impudicus.The UPGMA method of NTSYS 2.10e cluster analysis software was used for cluster analysis，and the ISSR statistics of 20 Phallus impudicus samples were analyzed by PopGen32,in order to evaluate the genetic diversity of Phallus impudicus germplasm resources by molecular markers and provide high quality materials for genetic breeding.The results showed that a total of 24 ISSR primers with polymorphism were screened out，and 788 ISSR bands were amplified by PCR，included 768 polymorphism bands，with the proportion of polymorphic loci reaching 97.5%；genetic diversity analysis showed that the average allele (Na) was 1.991 3，the average effective allele number (Ne) was 1.585 6，the average Nei′s gene diversity index (He) was 0.343 8，and the average Shannon information index (I) was 0.516 1.When the genetic similarity coefficient was 0.67，the 20 strains could be divided into three groups.In conclusion，there were abundant genetic diversity and complex genetic relationship among the 20 species.Due to the frequent circulation of germplasm from different sources，the genetic relationship was complicated，and the selection and purification cycle of breeding parents was long.

参考文献/References:

[1]郑秀艳,孟繁博,邬彩灵,等.黔产白鬼笔不同部位品质分析与评价[J].现代食品科技,2021,37(12):23-32.[2]李文力,黎璐,汤洪敏.黔产白鬼笔不同部位提取物的成分分析[J].食品科学,2016,37(2):72-76.[3]桂阳,龚光禄,卢颖颖,等.白鬼笔出菇特性研究[J].食用菌,2013,35(3):12-14.[4]宋笛.响应面优化微波辅助提取白鬼笔多糖及其抗氧化和抗糖化能力[J].食品研究与开发,2021,42(20):130-136.[5]易雪倩,徐宁,王小艳,等.一种鬼笔属真菌分离鉴定和挥发性成分分析[J].中国蔬菜,2022(7):78-84.[6]李永齐,王芬,廖头根,等.白鬼笔菌托多糖作为烟草保润剂的性能评价[J].食药用菌,2019,27(6):383-389.[7]UKO V,AKUNOVICH A,STOWSKI A,等.白鬼笔蘑菇菌丝体多糖：免疫调节和伤口愈合特性（英文）[J].现代食品科技,2019,35(9):30-37.[8]武海月,赵爽,刘宇,等.榆黄菇遗传多样性的ISSR和SRAP综合分析[J].生物技术通报,2018,34(4):121-126.[9]杨和川,苏文英,谭一罗,等.基于ISSR分子标记技术的杏鲍菇种质资源评价[J].西南农业学报,2019,32(7):1480-1486.[10]宋莹,刘俊杰,刘岩岩,等.辽宁省主栽香菇菌株ISSR遗传差异性分析[J].北方园艺,2017(5):82-85.[11]罗燕娜,王静静,李旭东,等.双孢蘑菇A15菌株的ISSR分子标记聚类分析[J].分子植物育种,2022,20(2):473-478.[12]柯丽娜,黄艺宁,袁滨,等.主栽毛木耳菌株农艺性状及ISSR遗传分析[J].福建农业学报,2021,36(7):771-778.[13]张彬彬,王震,刘保卫,等.30个野生灵芝菌株遗传多样性及农艺性状评价[J].江苏农业科学,2022,50(2):108-114.[14]汪茜,吴圣进,韦仕岩,等.金福菇菌株遗传多样性的ISSR分析[J].西南农业学报,2014,27(2):768-771.[15]王波,鲜灵.金针菇菌株遗传多样性的ISSR分析[J].西南农业学报,2013,26(4):1593-1599.[16]杨杰,张进强,周涛,等.蜜环菌和冬荪菌侵染与降解菌材的比较研究[J].中国中药杂志,2020,45(3):472-477.[17]张进强,江维克,郭兰萍,等.天麻种植旧菌材的资源再利用探索[J].中国中药杂志,2022,47(9):2309-2314.[18]刘鹏,曹永直,彭成江,等.利用天麻空窝和废菌材栽培白鬼笔技术[J].食用菌,2022,44(1):49-51.[19]王鸿磊,丁强,吕蔚,等.我国双孢菇菌株与国外引进菌株间亲缘关系的ISSR分析[J].北方园艺,2014(11):96-99.[20]贾培松,努尔孜亚?亚力买买提,罗影,等.新疆塔城地区野生阿魏菇菌株的遗传多样性分析[J].新疆农业科学,2016,53(10):1900-1906.[21]陈雪凤,郎宁,韦仕岩,等.8个反季节秀珍菇菌株栽培性状评价与遗传差异分析[J].西南农业学报,2019,32(8):1898-1903.[22]杨和川,苏文英,谭一罗,等.基于ISSR分子标记技术的杏鲍菇种质资源评价[J].西南农业学报,2019,32(7):1480-1486.[23]杨燕,田鸿,张小平,等.基于ITS和ISSR的羊肚菌种质资源遗传多样性分析[J].西南农业学报,2018,31(10):2004-2009.[24]谭艳,王波,赵瑞琳.金针菇菌株农艺性状评价与遗传差异分析[J].西南农业学报,2016,29(12):2965-2970.[25]王晓敏,吕瑞娜,李长田.应用SRAP、ISSR和TRAP标记构建金针菇分子遗传连锁图谱[J].分子植物育种,2020,18(13):4377-4383.[26]赵光辉,方洪枫,陈剑,等.20株草菇遗传多样性的分析[J].江苏农业科学,2020,48(16):194-197.

相似文献/References:

[1]肖志娟,翟梅枝,许静,等.不同核桃品种的ISSR标记分析[J].北方园艺,2013,37(04):103.
[2]刘志曦,李志强,魏海莲,等.平菇ISSR遗传多样性分析[J].北方园艺,2014,38(12):94.
　LIU Zhi-xi,LI Zhi-qiang,WEI Hai-lian,et al. Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker[J].Northern Horticulture,2014,38(14):94.
[3]李 娜,张存旭,崔晓燕,等.栓皮栎ISSR反应体系的建立[J].北方园艺,2014,38(13):93.
　LI Na,ZHANG Cun-xu,CUI Xiao-yan,et al.Establishment of ISSR Reaction System in Quercus variabilis [J].Northern Horticulture,2014,38(14):93.
[4]王鸿磊,丁 强,吕 蔚,等.我国双孢菇菌株与国外引进菌株间亲缘关系的ISSR分析[J].北方园艺,2014,38(11):96.
　WANG Hong-lei,DING qiang,LV Wei,et al.Genetic Relationship Based on ISSR Markers Among Agaricus bisporus Strains From China and Overseas[J].Northern Horticulture,2014,38(14):96.
[5]李 娜,张存旭,刘 伟,等.毛梾ISSR-PCR反应体系的建立[J].北方园艺,2013,37(22):114.
　LI Na,ZHANG Cun-xu,LIU Wei,et al.Establishment of ISSRPCR Reaction System of Cornus walteri[J].Northern Horticulture,2013,37(14):114.
[6]黄萌,陈尚钘,杨光耀,等.古樟ISSR扩增条件的优化[J].北方园艺,2013,37(07):116.
　HUANG Meng,CHEN Shang-xing,YANG Guang-yao,et al.Optimization of ISSR Amplification Conditions in Ancient Camphor-trees[J].Northern Horticulture,2013,37(14):116.
[7]吴春燕,高义,宋廷宇,等.正交设计优化大白菜ISSR-PCR 反应体系[J].北方园艺,2012,36(05):127.
　WU Chun-yan,GAO Yi,SONG Ting-yu,et al.Optimization of an ISSR-PCR Reaction System of Chinese Cabbage by Orthogonal Design[J].Northern Horticulture,2012,36(14):127.
[8]陈名红,李 玉,刘 多,等.适于ISSR-PCR扩增的百合基因组DNA的提取[J].北方园艺,2012,36(24):108.
　CHEN Ming-hong,LI Yu,LIU Duo,et al.Extraction of Genomic DNA for ISSR-PCR Reaction in Lilium[J].Northern Horticulture,2012,36(14):108.
[9]张运峰,胡芬.红掌组培再生植株中表型突变体的ISSR分析[J].北方园艺,2014,38(21):101.
　ZHANG Yun-feng,HU Fen.ISSR Analysis of Phenotypic Variants in Tissue Culturing Regeneration Plants of Anthurium andraeanum[J].Northern Horticulture,2014,38(14):101.
[10]孙叶迎,陈丽飞,刘树英,等.长白山区杓兰属植物ISSR-PCR最佳反应体系的建立[J].北方园艺,2013,37(24):92.
　SUN Ye-ying,CHEN Li-fei,LIU Shu-ying,et al.Establishment of the Optimum ISSR-PCR Reaction System of Cypripedium in the Region of Changbai Mountain of China[J].Northern Horticulture,2013,37(14):92.

备注/Memo

第一作者简介：卢颖颖（1985-），女，硕士，助理研究员，现主要从事食用菌育种与栽培等研究工作。Email：304321047@qq.com.责任作者：朱国胜（1971-），男，博士，研究员，现主要从事食用菌育种与栽培等研究工作。Email：49514224@qq.com.基金项目：贵州省科技计划资助项目（黔科合重大专项字[2019]3008，黔科合平台人才[2019]5105号，黔科合支撑[2019]2451-6，黔科合成果[2023]一般065，黔科合基础-ZK[2023一般172]）；贵州省科技支撑计划资助项目（黔科合支撑[2022]重点025号）；贵州省食用菌现代农业产业技术体系资助项目（GZCYTX2021-GZCYTX2025）。收稿日期：2022-11-17

更新日期/Last Update: 2023-08-29