BI Yange,LIANG Yufan,WANG Hui,et al.Analysis of Photosynthetic Characteristic and Chlorophyll Metabolism Related Genes Expression in Tomato Leaf Yellowing Mutant[J].Northern Horticulture,2023,(20):8-13.[doi:10.11937/bfyy.20224106]
番茄叶色黄化突变体叶片光合特性及叶绿素代谢相关基因的表达分析
- Title:
- Analysis of Photosynthetic Characteristic and Chlorophyll Metabolism Related Genes Expression in Tomato Leaf Yellowing Mutant
- 文章编号:
- 1001-0009(2023)20-0008-06
- Keywords:
- tomato; leaf color mutant; chlorophyll; photosynthetic rate
- 分类号:
- S 641.2
- 文献标志码:
- A
- 摘要:
- 以经甲基磺酸乙酯（ethyl methyl sulfone,EMS）诱变获得的番茄黄化突变体WAX-7及其野生型P31为试材，采用苗期对比的方法，研究了突变体与野生型主要形态特征、叶片光合相关色素、光合参数及叶绿素合成与代谢相关基因相对表达量之间的差异，以期为揭示番茄黄化突变体叶色发生变异的机制提供参考依据。结果表明：突变体WAX-7较其野生型表现为叶片黄化，株高、节间长度及叶面积均显著降低。突变体叶片中叶绿素a、叶绿素b、类胡萝卜素及叶绿素总含量均显著低于野生型；突变体叶片光合速率、胞间二氧化碳浓度及气孔导度均显著或极显著低于野生型。另外，突变体叶片中叶绿素合成关键基因SlPOR、SlCAO1、SlCHLH的相对表达量显著或极显著低于野生型，但叶绿素降解关键基因SlPPH的相对表达量却显著高于野生型。
- Abstract:
- Wild-type P31 of tomato and yellowing mutant WAX-7 obtained by ethyl methyl sulfone (EMS) mutagenesis were used as the main materials.Main morphological characteristics,leaf photosynthetic pigment content,photosynthetic parameters,and relative expression levels of chlorophyll synthesis and metabolism related genes between mutant and wild type were measured with method of comparison in seedling stage,in order to reveal the mechanisms underlying leaf color variation in tomato yellow mutants.The results showed which compared with the wild type，the mutant WAX-7 showed yellow leaves，the plant height，internode length and leaf area were significantly reduced.The contents of chlorophyll a，chlorophyll b，carotenoids and total chlorophyll in the leaves of the mutant were significantly lower than those of the wild type.In addition，the photosynthetic rate，intercellular carbon dioxide concentration and stomatal conductance of the mutant leaves were significantly or extremely significantly lower than those of the wild type.The relative expression levels of chlorophyll synthesis key genes SLPOR，SLCAO1，and SLCHLH in mutant leaves were also significantly or extremely significantly lower than those of wild type，but the relative expression of chlorophyll degradation key gene SLPPH was significantly higher than that of wild type.
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备注/Memo
第一作者简介：毕燕鸽（1997-），女，硕士研究生，研究方向为蔬菜遗传育种与分子生物学。E-mail：biyange97@163.com.责任作者：王富（1966-），男，博士，教授，现主要从事蔬菜遗传育种与生物技术等研究工作。E-mail：wangfuabcd@163.com.基金项目：山东省农业良种工程资助项目（2020LZGC005，2022LZGCQY001）；山东省现代农业产业技术体系资助项目（SDAIT-05）。收稿日期：2022-10-10