ZHOU Chao,MA Yinpeng,BAO Xuxiang,et al.Research Progress of DNA Molecular Marker Technology in Auricularia heimuer[J].Northern Horticulture,2023,(07):125-131.[doi:10.11937/bfyy.20223001]
DNA分子标记技术在黑木耳中的研究进展
- Title:
- Research Progress of DNA Molecular Marker Technology in Auricularia heimuer
- Keywords:
- Auricularia heimuer; DNA molecular marker; genetic diversity
- 文献标志码:
- A
- 摘要:
- 黑木耳（Auricularia heimuer）是我国食用菌产业中不可或缺的一个品种，同时分子生物学试验研究的热度与日俱增，尤其随着转录组学、蛋白组学、基因组学、生物信息学等分子生物学技术快速发展，分子标记技术已经广泛应用于黑木耳研究中。该研究概括了DNA分子标记技术的种类特点及应用，综述了近年来常用的DNA分子标记技术（ITS、SSR、ISSR、SRAP）在黑木耳遗传多样性与亲缘关系分析、遗传图谱构建等中的应用现状，总结了DNA分子标记技术在黑木耳研究中存在的问题,并对未来的发展趋势和应用前景进行了展望。
- Abstract:
- Auricularia heimuer is an indispensable variety in the edible fungus industry in China,and its molecular biology experimental research is increasingly hot,especially with the rapid development of molecular biology technologies such as transcriptome,proteomics,genomics,bioinformatics and so on,molecular marker technology has been widely used in Auricularia heimuer research.In this study,the characteristics and applications of DNA molecular markers were summarized,and the application status of DNA molecular markers (ITS,SSR,ISSR,SRAP) in the genetic diversity and genetic relationship analysis of Auricularia heimuer and the construction of genetic map were reviewed.The problems of DNA molecular markers of Auricularia heimuer were summarized,and prospected the development trend and application prospect in the future.
参考文献/References:
[1]吴芳,戴玉成.黑木耳复合群中种类学名说明[J].菌物学报,2015,34(4):604-611.[2]杨新美.中国食用菌[M].北京:中国农业出版社,1986.[3]吴芳,员瑗,刘鸿高,等.木耳属研究进展[J].菌物学报,2014,33(2):198-207.[4]ZHANG J X,CHEN Q.History,current situation and trend of edible fungi industry development[J].Mycosystema,2015(34):524-540.[5]朱海峰.黑木耳液体菌种老化评价指标的研究[D].哈尔滨:东北农业大学,2021.[6]黄莎,李伟荣,林芳,等.黑木耳营养和生物活性成分及其提取工艺研究进展[J].中国食用菌,2021,40(10):7-12.[7]朱文斌.黑木耳液体发酵及其多糖活性研究[D].合肥:安徽农业大学,2015.[8]李玉.中国黑木耳[J].长春:长春出版社,2001.[9]杨国良,刘化民.黑木耳DNA的提取及G-C含量测定[J].食用菌,1985(4):33-35.[10]徐丽芳,陈吉炎,罗光明.分子标记技术及其在植物育种中的应用[J].食品与药品,2007(10):43-46.[11]TEIXEIRADASILVA J A,JIN X H,DOBRANSZKI J,et al.Advancesin dendrobium molecular research:Applications in genetic variation,identification and breeding[J].Mol Phylogenet Evol,2016,95:196-216.[12]GRODZICKER T,WILLIAMS J,SHARP P,et al.Physical mapping of temperature-sensitive mutations of adenoviruses[J].Cold Spring Harbor Symposia on Quantitative Biology,1974,39:439-446.[13]BOTSTEIN D,WHITE R L,SKOLNICK M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3):314-331.[14]闫华超,高岚,李桂兰.分子标记技术的发展及应用[J].生物学通报,2006,41(2):17-19.[15]WILLIAMS J G,KUBELIK A R,LIVAK K J,et al.DNA polymorphisms amplified by arbitrary primer are useful as genetic marker[J].Nucleic Acids Research,1990,18(22):6531-6535.[16]WELSH J,MCCLELLAND M.Fingerprinting genomes using PCR with arbitrary primers[J].Nucleic Acids Research,1990,189(24):7213-7218.[17]MOORE S S,SARGEANT L L,KING T J,et al.The conservation of dinucleotide microsatellites among mammalian genomes allows the use of heterologous PCR primer pairs in closely related species[J].Genomics,1991,10(3):654-660.[18]ZABEAU M,VOS P,KEYGENE N V.Selective restriction fragment amplification：A general method for DNA finger printing[P].European：AU2662992A,1993-04-27.[19]KONIECZNY A,AUSUBEL F.A procedure for mapping arabidopsis mutations using codominantecotype-specific PCR-based markers[J].Plant Journal,1993,4(2):403-410.[20]TOURMENTE S,DERAGON J M,LAFLEURIEL J,et al.Characterization of minisatellites in Arabidopsis thaliana with sequence similarity ti the human minisatellite core sequence[J].Nucleic Acids Research,1994,22(16):3317-3321.[21]ZIETKIEWICZ E,RAFALSKI A,LABUDA D.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183.[22]VOS P,HOGERS R,BLECKER M.et al.AFLP:A new technique for DNA fingerprinting[J].Nucl Acids Res,1995,23(21):4407-4414.[23]LANDER E S.The new genomics：Global views of biology[J].Science,1996,274:536-539.[24]BUNYARD B A,CHAICHUCHOTE S,NICHOLSON M S,et al.Ribosomal DNA analysis for resolution of genotypic classes of Pleurotus[J].Mycol Res,1996,100(2):143-150.[25]李传友,伏健民,金德敏,等.AFLP 分析中多态性扩增产物的回收、克隆及鉴定[J].遗传,1998,20(4):1-4.[26]CHILLALI M,IDDER-IGHILI H,GUILAUMIN J J,et al.Variation in the ITS and IGS regions of ribosomal DNA among the biological species of European Armillaria[J].Mycological Research,1998,102(5):533-540.[27]LI G,QUIROS C F.Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction：Its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461.[28]王青山,李葱葱,王晶,等.AFLP分子标记技术及应用研究进展[J].吉林农业科学,2005,30(6):29-33.[29]牛宪立,姬可平,吴群,等.rDNA ITS区序列分子标记技术在植物学研究中的应用[J].生物信息学,2009,7(4):268-271.[30]杨晓兵,宋慧,刘麒,等.DNA分子标记在木耳属真菌中的应用研究进展[J].菌物研究,2010,8(3):181-186.[31]YUE J,ZHU Z C.Optimization of SSR reaction system for identifying the authenticity of maize hybrids[J].Agricultural Science&Technology,2011,12(1):85-87.[32]张金霞.中国食用菌菌种学[M].北京：北京中国农业出版社,2011.[33]陈星,高子厚.DNA分子标记技术的研究与应用[J].分子植物育种,2019,17(6):1970-1977.[34]吴晓雯,王铁杆,刘颖,等.DNA分子标记技术在坛紫菜(Pyropia haitanensis)中的应用[J].渔业研究,2020,42(3):281-287.[35]廖秋石,雷刚,陈学军,等.分子标记技术在茄子种质资源评价中的应用研究进展[J].江西农业学报,2020,32(8):44-51.[36]彭枫,张莹,陈珂,等.DNA分子标记技术在菠菜遗传育种中的应用研究进展[J].上海师范大学学报(自然科学版),2021,50(2):237-242.[37]高雅迪,张振豪,王一帆,等.DNA分子标记技术在菊花种质资源中的应用研究[J].杭州师范大学学报(自然科学版),2022,21(2):152-157.[38]员瑗.中国野生黑木耳遗传多样性及全基因组信息分析[D].北京:北京林业大学,2018.[39]王进,陈文强,邓百万,等.基于形态特征和ITS序列分析秦巴山区黑木耳主要栽培种的亲缘关系[J].食品与生物技术学报,2014,33(5):535-541.[40]宋君,王东,郭灵安,等.利用DNA条码对农产品产地溯源研究一黑木耳产地分子溯源[J].分析试验室,2014,33(3):292-295.[41]刘虹,杨杰,郭丽杰,等.基于nrDNA-ITS片段的16份木耳属物种鉴定[J].山西农业大学学报(自然科学版),2020,40(4):36-43.[42]张姣.黑木耳优良菌株选育及富硒栽培技术研究[D].杨凌:西北农林科技大学,2021.[43]钱雪婷,陈文强,邓百万,等.基于ITS序列分析秦巴山区黑木耳菌株的遗传多样性[J].北方园艺,2016(12):147-151.[44]张耀玲,陈荣信,荆丹,等.秦巴山区黑木耳遗传多样性初探[J].陕西农业科学,2021,67(9):24-27.[45]潜宗伟,陈海丽,崔彦玲.茄子种子纯度 SSR 标记快速鉴定[J].北方园艺,2014(23):75-78.[46]冯立健.黑龙江野生黑木耳种质资源的SSR分析[D].哈尔滨:东北农业大学,2014.[47]周雁,范秀芝,陈连福,等.SSR在黑木耳和毛木耳转录组中的分布和序列特征[J].菌物学报,2014,33(2):280-288.[48]马银鹏,陈鹤,孔祥辉,等.黑龙江省黑木耳主栽品种SSR遗传多样性初探[J].食用菌,2016,38(5):17-18.[49]徐安然,付永平,王延锋,等.黑木耳部分种质资源SSR分子身份证的构建[J].农业生物技术学报,2017,25(12):1930-1939.[50]初赛君.黑木耳野生种质资源多样性的研究[D].吉林:吉林农业大学,2018.[51]李晶晶,姚方杰,鲁丽鑫.黑木耳自交菌株菌丝体的遗传多样性分析[J/OL].吉林农业大学学报,(2021-08-02)[2023-02-08].https://doi.org/10.13327/j.jjlau.2021.1220.[52]潘春磊,王延锋,盛春鸽,等.黑木耳59个菌株的遗传多样性分析[J].中国食用菌,2022,41(1):17-22.[53]LU L X,YAO F J,WANG P,et al.Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae[J].Journal of Microbiology,2017,55(10):792-799.[54]路新彦,刘昆,蒋俊,等.7个黑木耳菌株遗传差异性分析[J].中国食用菌,2017,36(1):52-55.[55]李红,张敏,屈麟,等.基于ISSR-PCR分子标记的黑木耳遗传多样性分析[J].园艺与种苗,2019,39(6):1-3.[56]马银鹏,包怡红,张丕奇,等.黑龙江东宁境内栽培黑木耳菌株区别性鉴定[J].食用菌,2019,41(4):28-30.[57]宋吉玲,陆娜,闫静,等.黑木耳菌株农艺性状评价与遗传差异分析[J].西北农林科技大学学报(自然科学版),2021,49(3):147-154.[58]DU P,CAO T X,CUI B K,et al.Genetic diversity and relationships of 24 strains of genus Auricularia (Agaricomycetes) assessed using SRAP markers[J].Int J Med Mushrooms.2016;18(10):945-954.[59]姚方杰,鲁丽鑫,王鹏,等.SRAP法鉴定黑木耳菌种真实性[J].北方园艺,2017(24):178-182.[60]史灵燕,张云龙,刘保卫,等.基于SRAP分子标记的24个黑木耳栽培菌株遗传分析[J].食用菌,2019,41(6):20-23.[61]刘昆昂,马宏,刘萌,等.采用r DNA-ITS和SRAP综合分析黑木耳栽培种的遗传多样性[J].分子植物育种,2021,19(24):8223-8232.[62]李黎.中国木耳栽培种质资源的遗传多样性研究[D].武汉:华中农业大学,2011.[63]LI L,ZHONG C H,BIAN Y B.The molecular diversity analysis of Auricularia auricula-judae in China by nuclear ribosomal DNA intergenic spacer[J].Electronic Journal of Biotechnology,2014,17(1):27-33.[64]李媛媛,隋玉龙,牛淑力,等.SCAR标记在黑木耳栽培菌株分类鉴定中的应用[J].菌物研究,2013,11(3):182-185.[65]范秀芝,周雁,边银丙.基于等位基因InDel快速检测黑木耳原生质体单核体[J].菌物学报,2014,33(2):273-279.[66]韩增华,张介驰,张丕奇,等.黑木耳单核杂交菌株遗传分析[J].核农学报,2015,29(5):843-848.[67]张琳.香菇基于RNA-Seq构建遗传连锁图及重要性状的QTL定位[D].武汉:华中农业大学,2018.[68]章炉军,高成,于海龙,等.香菇F2代群体农艺性状的SSR关联分析[J].食用菌学报,2019,26(1):1-10.[69]刘伟,张倩倩,舒芳,等.梯棱羊肚菌全基因组SNP/Indel分析及基于Indel标记的遗传连锁图谱构建[J].菌物学报,2019,38(12):2195-2204.[70]肖冬来,张迪,林衍铨,等.20株香菇菌株的ISSR和F-MSAP分析[J].福建农业学报,2018,33(8):794-798.[71]冯志勇,陈辉,汪虹,等.基于交配型基因的酶切扩增多态性序列鉴定双孢蘑菇核型[J].食用菌学报,2019,26(4):9-15.[72]马杰,屈雯,陈春艳,等.基于转录组序列的羊肚菌EST-SSR标记开发与遗传多样性分析[J].江苏农业学报,2020,36(5):1282-1290.[73]果禹鑫,陈青君,张国庆,等.运用交配型基因分子标记辅助羊肚菌单孢杂交育种[J].分子植物育种,2022,20(12):4067-4074.[74]姚方杰,张友民,鲁丽鑫,等.黑木耳遗传育种研究进展[J].菌物研究,2015,13(3):125-128.
相似文献/References:
[1]桂明英,赵春艳,吴素蕊,等.黑木耳18S rDNA序列变异及遗传多样性分析[J].北方园艺,2014,38(14):111.
GUI Ming-ying,ZHAO Chun-yan,WU Su-rui,et al.Analyses of Sequence Variation and Genetic Diversity on 18S rDNA Regions of Auricularia auricula-judae[J].Northern Horticulture,2014,38(07):111.
[2]徐彦军,吴秀泉,宋世良,等.不同速生树种培养料对黑木耳生长及产量的影响[J].北方园艺,2013,37(10):143.
XU Yan-jun,WU Xiu-quan,SONG Shi-liang,et al.Effect of Different Culture Material of Fast growing Trees on the Growth and Yield of Auricularia auricula[J].Northern Horticulture,2013,37(07):143.
[3]陈 影,姚方杰,张友民,等.黑木耳新品种选育研究[J].北方园艺,2014,38(08):133.
CHEN Ying,YAO Fang-jie,ZHANG You-min,et al.Study on Breeding of A New Auricularia auricula-judae (Bull.) Quél.Cultivar[J].Northern Horticulture,2014,38(07):133.
[4]何彩梅,唐政.黑木耳多糖的提取工艺研究[J].北方园艺,2013,37(07):156.
HE Cai-mei,TANG Zheng.Study on Extraction Technology of Polysaccharides from Auricularia auricula[J].Northern Horticulture,2013,37(07):156.
[5]唐玉琴,李凤林,宫敬利.黑木耳新品种“吉耳1号”的区域试验[J].北方园艺,2012,36(02):174.
TANG Yu-qin,LI Feng-lin,GONG Jing-li.Regional Trials of New Variety of Auricularia auricula ‘Jier No1’[J].Northern Horticulture,2012,36(07):174.
[6]魏雅冬,戴明,王相刚,等.黑木耳固体栽培基质优化研究[J].北方园艺,2013,37(16):176.
WEIYa-dong,DAIMing,WANGXiang-gang,et al.StudyonOptimizationoftheSolidSubstratesofBlackFungus[J].Northern Horticulture,2013,37(07):176.
[7]王鹏,郭丽,周凤超,等.黑木耳提取液流变学性质研究[J].北方园艺,2013,37(24):146.
WANG Peng,GUO Li,ZHOU Feng-chao,et al.Study on Rheological Properties of Black Fungus Extract[J].Northern Horticulture,2013,37(07):146.
[8]唐玉琴.通过原生质体技术选育的黑木耳新品种“双高一号”品比研究[J].北方园艺,2014,38(24):153.
TANG Yu-qin.Variety Comparison Test of New Black Fungus ‘Shuanggao No.1’ That Breeding Through Protoplast Technology[J].Northern Horticulture,2014,38(07):153.
[9]王伟,刘颖,韩光,等.秸秆替代阔叶木屑代料栽培黑木耳的应用研究[J].北方园艺,2015,39(08):143.[doi:10.11937/bfyy.201508037]
WANG Wei,LIU Ying,HAN Guang,et al.Study on Substitute Cultivation Auricularia auricular with Straw[J].Northern Horticulture,2015,39(07):143.[doi:10.11937/bfyy.201508037]
[10]王相刚,许修宏,缪元霞,等.农作物秸秆替代木屑栽培黑木耳的关键性技术[J].北方园艺,2015,39(05):160.[doi:10.11937/bfyy.201505046]
WANG Xiang-gang,XU Xiu-hong,MIAO Yuan-xia,et al.The Key Technology of Black Fungus Cultivation Using Crop Straw Instead of Sawdust[J].Northern Horticulture,2015,39(07):160.[doi:10.11937/bfyy.201505046]
备注/Memo
第一作者简介：周超（1996-），男，硕士研究生，研究方向为黑木耳栽培与遗传育种。E-mail：zhouchao9292@163.com.责任作者：张介驰（1971-），男，硕士，研究员，现主要从事黑木耳栽培与遗传育种等研究工作。E-mail：ltzjc@sina.com.基金项目：国家现代农业产业技术体系专项资金资助项目（CARS-20）；黑龙江省科学院双提雁阵特别计划资助项目（YZ202002）。收稿日期：2022-07-21