YANG Chaosha,WANG Shanshan,YIN Weiping,et al.Detection of Six Disease Resistance Genes in Eighty-one Tomato Germplasm Resources[J].Northern Horticulture,2022,(20):1-9.[doi:10.11937/bfyy.20215153]
八十一份番茄种质资源的六种病害抗病基因检测
- Title:
- Detection of Six Disease Resistance Genes in Eighty-one Tomato Germplasm Resources
- Keywords:
- tomato; disease resistance genes; penta-primer amplification refractory mutation system（PARMS）; multiple genes pyramiding
- 文献标志码:
- A
- 摘要:
- 以课题组选育的81份大果番茄自交系为试材，利用PARMS技术对黄化曲叶病毒病抗病基因Ty-1、Ty-2、Ty-3/3a，根结线虫病抗病基因Mi-1.2、冠状根腐病抗病基因Frl、叶霉病抗病基因Cf-5、Cf-9、晚疫病抗病基因Ph-3、灰叶斑病抗病基因Sm进行了检测，分析了目前这些材料中多抗基因的种类及分布，以期为聚合育种提供参考依据。结果表明：81份番茄材料中，含黄化曲叶病毒病抗病基因（Ty-1或Ty-2）、叶霉病抗病基因（Cf-5或Cf-9）、灰叶斑病抗病基因、根结线虫病抗病基因的材料较多，分别为76、68、66、65份；含4个抗病基因的材料最多，共31份。结合田间表现，最终筛选出3个含有6个抗病基因的纯合材料463、杂合材料398、354，及含有5个抗病基因的材料409、470、479、480。下一步将对筛选出的材料进行纯化，为选育多抗优质番茄新品种奠定基础。
- Abstract:
- Penta-primer amplification refractory mutation system （PARMS） were used to test for tomato yellow leaf curl virus disease resistant genes Ty-1、Ty-2、Ty-3/3a，root knot nematode resistant gene Mi-1.2，Fusarium crown and root rot resistance gene Frl，leaf mould resistant genes Cf-5、Cf-9，late blight resistant gene Ph-3 and gray leaf spot resistant gene Sm in 81 tomato inbred lines to clarify the types and distribution of multi-resistant genes in these materials,in order to provide reference for the screening of multi-resistant tomato germplasm resources.The results showed that there were 76，68，66 and 65 materials with the tomato yellow leaf curl virus disease resistant genes Ty-1 or Ty-2，leaf mould resistant genes Cf-5 or Cf-9，gray leaf spot resistant gene Sm，root knot nematode resistant gene Mi-1.2 respectively.A total of 31 materials containing four disease resistance genes at most.Combined with the field performance，three homozygous materials 463，heterozygous materials 398 and 354 with six disease resistance genes and materials 409，470，479 and 480 with five disease resistance genes were selected.The next step was to purify the selected materials，which would lay a foundation for breeding new tomato varieties with multi resistance and high quality.
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备注/Memo
第一作者简介：杨超沙（1989-），女，硕士，助理研究员，现主要从事番茄育种及栽培等研究工作。E-mail：chshyang126@126.com.责任作者：尹庆珍（1967-），女，硕士，研究员，现主要从事番茄育种及栽培等研究工作。E-mail：yinqingzhen67@163.com.基金项目：河北省重点研发计划资助项目（21326309D-3，22326313D）；河北省农林科学院财政资助项目（2022KJCXZX-JZS-5）；现代农业产业体系河北省创新团队建设专项资助项目（HBCT2018030204）；河北省第三批“巨人计划”-蔬菜科研创新团队资助项目；石家庄市科技计划资助项目（2490092A）。收稿日期：2021-12-17