MA Jian,DONG Ruichu,CHEN Mengjiao,et al.Analysis of Fruit Setting and Phytohormone Content of a Low-temperature Tolerant Tomato Variety LA2006 Under Cold Stress[J].Northern Horticulture,2022,(10):49-55.[doi:10.11937/bfyy.20214233]
耐低温番茄品系LA2006低温逆境下坐果能力及花器官激素分析
- Title:
- Analysis of Fruit Setting and Phytohormone Content of a Low-temperature Tolerant Tomato Variety LA2006 Under Cold Stress
- Keywords:
- tomato; low temperature; fruit setting; phytohormone
- 文献标志码:
- A
- 摘要:
- 以低温下坐果能力强的品系LA2006及不耐低温的DR5品系为试材，通过18 ℃/4 ℃低温处理试验，研究了低温逆境对2个品系坐果率及花器官相关内源激素含量的影响，以期明确耐低温品系L2006低温下坐果的生理机制，为低温逆境下设施番茄栽培调控提供参考依据。结果表明：LA2006番茄在低温18 ℃/4 ℃处理下坐果率达20.6%，而对照DR5无坐果；游离态生长素IAA在2个品系的花器官中含量无显著差异，但LA2006花器官中结合态的生长素含量均有升高趋势；LA2006番茄活性赤霉素GA1含量显著下降，而其前体物质GA19、GA53含量显著上升；LA2006番茄的反式玉米素核苷（tZR）含量显著低于DR5，但二氢玉米素（DZ）、异戊烯腺嘌呤（IP）、顺式玉米素核苷（cZR）含量则显著高于DR5；LA2006番茄中ABA含量显著高于DR5，含量为DR5的1.46倍；2个品系的乙烯合成前体ACC含量无显著差异。综上所述，结合生长素、二氢玉米素（DZ）与脱落酸可能在LA2006低温坐果过程中发挥着重要作用。
- Abstract:
- LA2006 strain with strong fruit-setting ability at low temperature and DR5 with sensitized to cold stresses were used as test materials under 18 ℃/4 ℃ temperature treatment.The effects of low temperature stress on the fruit setting rate and the content of endogenous hormones related to flower organs of two strains were studied,in order to clarify the fruit setting mechanism of low temperature tolerant strain L2006 under low temperature,and provide reference for the regulation of protected tomato cultivation under low temperature stress.The results showed that LA2006 had a fruit setting of 20.6% at a low temperature of 18 ℃/4 ℃,while the control DR5 had no fruit setting.There was no significant difference in the content of free auxin IAA in the floral organs of the two lines,but all the combined states were even higher in the LA2006 floral organs.The content of active gibberellin GA1 decreased significantly in LA2006,while the content of precursor substances GA19 and GA53 increased significantly.At the same time,trans-zeatin nucleoside (tZR) was significantly lower in LA2006 compared with DR5,while dihydrozein (DZ),isopentenyl adenine (IP),and cis-zeatin nucleoside (cZR) were significantly higher than that of DR5.It was also found that the ABA content in LA2006 was 1.46 times of DR5.There was no significant difference in the content of ACC,the ethylene synthesis precursor.The above results indicated that conjugated auxin,dihydrozeatin (DZ) and abscisic acid would play the crucial role for fruit setting of LA2006 under low-temperature stress.
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备注/Memo
第一作者简介：马健(1973-)，男，本科，副研究员，现主要从事设施蔬菜栽培生理等研究工作。E-mail：mj76@163.com.责任作者：齐明芳(1976-)，男，博士，教授，现主要从事设施蔬菜栽培生理等研究工作。E-mail：qimingfang@126.com.基金项目：国家重点研发计划资助项目（2019YFD1001902）。收稿日期：2021-10-18