[1]佘晓环,张梓豪,李明.穿心莲四倍体的诱导及其愈伤组织对肉桂酸胁迫的响应[J].北方园艺,2022,(02):94-103.[doi:10.11937/bfyy.20213211]
　SHE Xiaohuan,ZHANG Zihao,LI Ming.Polyploidy Induction of Andrographis paniculata （Burn.F.） Nees and Its Response to Cinnamic Acid[J].Northern Horticulture,2022,(02):94-103.[doi:10.11937/bfyy.20213211]
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穿心莲四倍体的诱导及其愈伤组织对肉桂酸胁迫的响应

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年02 页码: 94-103 栏目: 出版日期: 2022-01-30
Title:
Polyploidy Induction of Andrographis paniculata （Burn.F.） Nees and Its Response to Cinnamic Acid
作者:
佘晓环1; 张梓豪1; 李明1; 2
（1.广东药科大学 中药学院，广东 广州 510006；2.国家中医药管理局岭南药材生产与开发重点研究室，广东 广州 510006）
Author(s):
SHE Xiaohuan1; ZHANG Zihao1; LI Ming1; 2
(1.College of Chinese Medicine，Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006;2.Key Laboratory of Production and Development of Lingnan Medicinal Materials,State Administration of Traditional Chinese Medicine,Guangzhou,Guangdong 510006)
关键词:
穿心莲; 多倍体; 肉桂酸; 抗性
Keywords:
Andrographis paniculata （Burn.F.） Nees; polyploid; cinnamic acid; resistance
DOI:
10.11937/bfyy.20213211
文献标志码:
A
摘要:
以不同倍性穿心莲诱导的愈伤组织为试材，采用秋水仙素对穿心莲种子进行四倍体诱导，通过在穿心莲四倍体及二倍体诱导愈伤组织培养基中添加化感自毒物质肉桂酸进行共培养的方法，研究了肉桂酸对不同倍性穿心莲愈伤组织的褐化率、细胞鲜质量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量的影响，以期为筛选穿心莲抗化感自毒物质的优良种质资源提供参考依据。结果表明：用0.05%的秋水仙素处理48 h，穿心莲种子诱导成活率达89%，经过植株形态、气孔及染色体数目检测，确定了穿心莲四倍体株系PZ-1、PZ-2、PZ-3、PZ-4、PZ-5、PZ-6、PZ-7、PZ-8，其染色体数为4x=96，二倍体为2x=48。在1.00 mmol·L-1肉桂酸的愈伤组织诱导培养基中培养7 d，穿心莲四倍体愈伤组织的褐化率和MDA含量较二倍体低，而愈伤组织鲜质量、SOD、POD、CAT活性较二倍体高，显示四倍体较二倍体具有对肉桂酸更强的抵御能力，其中四倍体PZ-7表现出较显著的优异性状。
Abstract:
The callus induced by different ploidy of Andrographis paniculata （Burn.F.） Nees was used as the test material,and colchicine was used to induce tetraploidy of Andrographis paniculata （Burn.F.） Nees seeds by adding allelopathic autotoxic substances to the culture medium for the callus induction of tetraploid and diploid of Andrographis paniculata （Burn.F.） Nees.The effects of cinnamic acid on the browning rate,cell fresh weight,superoxide dismutase (SOD),peroxidase (POD),catalase (CAT) activity and malondialdehyde (MDA) content of callus of different ploidy Andrographis paniculata （Burn.F.） Nees.,in order to provide a preliminary research foundation for screening excellent germplasm resources of Andrographis paniculata （Burn.F.） Nees against allelopathic and autotoxic substances.The results showed that treated with 0.05% colchicine for 48 hours,the induced survival rate of Andrographis paniculata （Burn.F.） Nees seeds reached 89%.After the plant morphology,stomata and chromosome number were tested,the tetraploid lines PZ-1,PZ-2,and PZ-3 of Andrographis paniculata （Burn.F.） Nees were determined,PZ-4,PZ-5,PZ-6,PZ-7,PZ-8,the number of chromosomes was 4x=96,and the diploid was 2x=48.After culturing in 1.00 mmol·L-1 cinnamic acid callus induction medium for 7 days,the browning rate and MDA content of Andrographis paniculata （Burn.F.） Nees tetraploid callus were lower than those of diploid,while the fresh quality of callus,SOD,POD,CAT activities were higher than those of diploid,which showed that tetraploid had stronger resistance to cinnamic acid than diploid.Among them,tetraploid PZ-7 showed more remarkable excellent characteristics.

参考文献/References:

[1]LI Z H,WANG Q,XIAO R,et al.Phenolics and plant allelopathy[J].Molecules,2010,15(12):8933-8952.[2]GUENZI W D,MCCALLA T M.Phenolic acids in oats,wheat,sorghum,and corn residues and their phytotoxicity1[J].Agronomy Journal,1966,58(3):303-304.[3]YU J Q,MATSUI Y.Phytotoxic substances in root exudates of cucumber (Cucumis sativus L.)[J].Journal of Chemical Ecology,1994,20(1):21-31.[4]BOOKER F L,BLUM U,FISCUS E L.Short-term effects of ferulic acid on ion uptake and water relations in cucumber seedlings[J].Journal of Experimental Botany,1992,43(5):649-655.[5]POLITYCKA B,KUBIS〖JX-+0.8mm〗〖KG-*2〗＇ J.Changes in free polyamine level and polyamine oxidase activity in cucumber roots under allelochemical stress conditions[J].Acta Physiologiae Plantarum,2000,22(1):11-16.[6]LIU D L,LOVETT J V.Biologically active secondary metabolites of barley.Ⅱ.Phytotoxicity of barley allelochemicals[J].Journal of Chemical Ecology,1993,19(10):2231.[7]HEJL A A M,EINHELLIG F A,RASMUSSEN J A.Effects of juglone on growth,photosynthesis,and respiration[J].Journal of Chemical Ecology,1993,19(3):559-568.[8]聂铭.不同连作年限地黄生长生理特性及其根区土壤化感物质研究[D].新乡：河南农业大学,2017.[9]EINHILLIG F A.Mechanism of action of allelochemicals in allelpothy[J].Allelopathy,1995(1):97-115.[10]ABD ELRAHMAN S S,MAZEN M M,MOHAMED H I,et al.Induction of defence related enzymes and phenolic compounds in lupin (Lupinus albus L.) and their effects on host resistance against Fusarium wilt[J].European Journal of Plant Pathology,2012,134(1):105-116.[11]张金燕,孙雪婷,陈军文,等.连作三七根际土壤化感物质检测及其提取液对三种作物种子萌发的影响[J].南方农业学报,2017,48(7):1178-1184.[12]张璐.连作甜瓜土壤中酚酸类物质的变化规律和木霉菌降解作用研究[D].沈阳：沈阳农业大学,2017.[13]王宁,余晓光,李卫军,等.四种化感物质对苜蓿和菟丝子种子萌发及幼苗生长的影响[J].新疆农业科学,2008(5):875-879.[14]宋慧,屈洋,张盼盼,等.肉桂酸和邻苯二甲酸对小豆根系生长和产量的自毒效应[J].西北农业学报,2016,25(10):1494-1501.[15]陈绍莉.茄子连作障碍的主要化感物香草醛和肉桂酸的作用机理及嫁接调节[D].沈阳：沈阳农业大学,2015.[16]YAMADA T,ICHIMURA K,DOORN W G.Relationship between petal abscission and programmed cell death in Prunus yedoensis and Delphinium belladonna[J].Planta,2007,226(5):1195-1205.[17]杨莉,韦美丽,黄天卫,等.用气孔保卫细胞叶绿体鉴定三七多倍体方法初探[J].现代中药研究与实践,2015,29(3):15-17.[18]马婷玉,李明,吴燕燕,等.穿心莲染色体制片优化及核型分析[J].江苏农业科学,2017,45(7):33-36.[19]蔡旭.植物遗传育种学[M].2版.北京:科学出版社,1996.[20]杨振华.秋水仙素处理甘草种子染色体加倍研究[J].山西农业科学,2016,44(3):294-296.[21]白英豪,张晓丽,李明军.秋水仙素诱导丹参多倍体研究进展[J].江苏农业科学,2018,46（5):18-20.[22]于辉,申志英,任亚超.种子浸渍法诱导黄芩多倍体的探索[J].种子,2011,30(5):91-94.[23]李世民,粱可钧,葛传吉,等.怀牛膝多倍体育种的研究[J].中药通报,1998,13(7):395.[24]HUANG H P,GAO S L,CHEN L L,et al.In vitro induction and identification of autotet raploids of Dioscoreazingiber ensis[J].In Vitro Cell Deve Biol Plant,2008,44:448-455.[25]GAO S L,CHEN B J,ZHU D N.In vitro production and identification of autotetraploids of Scutellaria baicalensis[J].Plant Cell Tiss Org Cult,2002,70:289-293.[26]李彬,陈万生,赵阳,等.四倍体板蓝根中的苯丙素类成分[J].中草药,2005,36(3):326-328.[27]段英姿,客绍英,曹静,等.秋水仙碱诱导南丹参多倍体研究[J].中国中药杂志，2006(6):445-448.[28]王小华,熊丽,屈云慧,等.中国桔梗多倍体诱导与鉴定[J].云南植物研究,2006(6):593-598.[29]YU J Q,MATSUI Y.Phytotoxic substances in root exudates of cucumber (Cucumis sativus L.)[J].Journal of Chemical Ecology,1994,20(1):21-31.[30]ZENG R S,LUO S M,SHI Y H,et al.Physiological and biochemical mechanism of allelopathy of secalonic acid on higher plants[J].Agronomy Journal,2001,93(1):72-79.[31]战宇,张连学,孟祥茹,等.不同盆栽人参土壤酚酸含量及酶活性变化研究[J/OL].吉林农业大学学报.https://doi.org/10.13327/j.jjlau.2020.5987.(2020-12-18)[2021-06-10].[32]LI P D,WANG X X,LI Y L,et al.The contents of phenolic acids in continuous cropping peanut andtheir allelopathy[J].Acta Ecologica Sinica,2010,30(8):2128-2134.[33]吴凤芝.外源酚酸对黄瓜自毒作用的生理生化机制研究[D].哈尔滨：东北农业大学,2002.[34]陈龙,张美玲,辛明月,等.外源酚酸对盆栽大豆苗期生长发育影响研究[J].中国农学通报,2014,30(24):129-132.[35]唐堃,李明,赵盼,等.广藿香连作土壤对其扦插苗扦插生根的化感作用[J].广东农业科学,2014,41(6):85-88，237.[36]张韵.黄瓜自毒物质对细胞生长的影响及缓解机制[D].杭州：浙江大学,2009.[37]尚玥,刘韬,吴丽军,等.不同倍性小麦对盐胁迫的适应性差异[J].广西植物,2017,37(12):1560-1571.[38]张笑艺.盾叶薯蓣四倍体诱导及高温抗性研究[D].武汉：华中农业大学,2009.

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备注/Memo

第一作者简介：佘晓环（1996-），女，硕士研究生，研究方向为中药学。E-mail：1007592205@qq.com.责任作者：李明（1963-），女，博士，教授，现主要从事中药资源品质评价及开发利用等研究工作。E-mail：13539843803@163.com.基金项目：广东省科技计划资助项目（2014A020208135）；国家重点研发计划资助项目（2017YFC1700704）；广东省重点领域研发计划资助项目（2020B020221002）；广东省普通高校重点领域专项资助项目（2020ZDZX1055）。收稿日期：2021-08-05

更新日期/Last Update: 2022-04-08