[1]李海炎,阳爱民,梁瑞郑,等.不同需冷量桃休眠相关DAM基因表达特性及激素调控的响应分析[J].北方园艺,2022,(03):27-34.[doi:10.11937/bfyy.20213198]
　LI Haiyan,YANG Aimin,LIANG Ruizheng,et al.Analysis of Dormant-related DAM Gene Expression Characteristics and Hormonal Regulation Responses in Different Chilling Peaches[J].Northern Horticulture,2022,(03):27-34.[doi:10.11937/bfyy.20213198]
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不同需冷量桃休眠相关DAM基因表达特性及激素调控的响应分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2022年03 页码: 27-34 栏目: 出版日期: 2022-02-15
Title:
Analysis of Dormant-related DAM Gene Expression Characteristics and Hormonal Regulation Responses in Different Chilling Peaches
作者:
李海炎; 阳爱民; 梁瑞郑; 陆泰良; 刘凤琼; 万保雄
（广西特色作物研究院，广西 桂林 541004）
Author(s):
LI Haiyan; YANG Aimin; LIANG Ruizheng; LU Tailiang; LIU Fengqiong; WAN Baoxiong
(Guangxi Academy of Specialty Crops,Guilin,Guangxi 541004)
关键词:
桃; 休眠; DAM基因; 激素; 表达特性
Keywords:
peach; dormancy; DAM gene; hormone; expression characteristics
DOI:
10.11937/bfyy.20213198
文献标志码:
A
摘要:
以“南山甜桃”（需冷量200 h）、“瑞光51号”（需冷量400 h）和“春美”（需冷量600 h）为试材，从大量落叶期开始至花芽萌动期间，约每15 d采样一次，RT-PCR分析DAM基因在不同冷量积累时期花芽中的表达量，PLC-MS-MS测定相同时期吲哚乙酸（IAA）、脱落酸（ABA）和玉米素（ZT）含量变化，以期了解不同需冷量桃休眠过程中DAM基因的表达特性及激素的调控机理。结果表明：DAM基因在不同需冷量桃休眠过程中的表达趋势一致，随着休眠的深入，“春美”中DAM基因的表达水平呈先升后降趋势；“南山甜桃”和“瑞光51号”在进入休眠期以后DAM基因表达水平急剧下降，与低温积累量呈负相关。“春美”中DAM基因表达量明显高于“瑞光51号”和“南山甜桃”。“春美”中DAM基因表达水平比“南山甜桃”和“瑞光51号”开始下调约晚30 d，但“瑞光51号”和“南山甜桃”中DAM基因下调的时间一致。IAA、ZT在不同需冷量桃休眠过程中的含量变化差异不显著，ABA含量变化差异显著，可能在休眠解除过程中起重要调控作用。
Abstract:
‘Nanshan Tiantao’(CR 200 hours),‘Ruiguang No.51’(CR 400 hours) and ‘Chunmei’(CR 600 hours) were taken as materials and sampled about every half a monthfrom the beginning of a large number of deciduous periods to the period of flower bud sprouting.The expression levels of DAM genes in flower buds during different cold accumulation periods were analyzed by RT-PCR.The contents of IAA,ABA and ZT in the same period were determined by PLC-MS-MS,in order to understand the expression characteristics of DAM genes and the regulation mechanism of hormones during the dormancy of different chilling peaches.The results showed that the expression trend of DAM genes was consistent in the dormancy of different chilling peaches.With the deepening of dormancy,the expression level of DAM genes in ‘Chunmei’ increased first and then decreased;the expression level of DAM genes in ‘Nanshan Tiantao’ and ‘Ruiguang No.51’ decreased sharply,which was negatively correlated with the accumulation of low temperature.The expression level of DAM genes in ‘Chunmei’ was significantly higher than that in ‘Nanshan Tiantao’ and ‘Ruiguang No.51’,and the expression level began to be down-regulated about one month later than the ‘Ruiguang No.51’ and ‘Nanshan Tiantao’,but ‘Ruiguang No.51’ and ‘Nanshan Tiantao’ had the same down-regulation time.The content of IAA and ZT was no significant difference during the dormancy of different chilling peaches,but the content of ABA was significant difference,which may play an important role in the process of dormancy release.

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备注/Memo

第一作者简介：李海炎（1987-），女，硕士，助理研究员，现主要从事果树分子生物学等研究工作。E-mail:13949043327@163.com.责任作者：万保雄（1977-），男，硕士，副研究员，现主要从事果树栽培等研究工作。E-mail:wan77118@163.com.基金项目：财政部和农业农村部：国家现代农业产业技术体系资助项目（CARS-30）；广西特色作物试验站资助项目（桂TS202108）。收稿日期：2021-08-04

更新日期/Last Update: 2022-04-09