JIN Baoxia,WEI Xiaohong,ZHU Xiaolin,et al.Cloning,Bioinformatics and Expression Analysis of ERFb2 Transcription Factor in Tomato[J].Northern Horticulture,2021,(23):1-10.[doi:10.11937/bfyy.20212435]
番茄ERFb2转录因子的克隆、生物信息学及表达特征分析
- Title:
- Cloning,Bioinformatics and Expression Analysis of ERFb2 Transcription Factor in Tomato
- Keywords:
- tomato; ERFb2; bioinformatics; expression analysis
- 文献标志码:
- A
- 摘要:
- 通过克隆番茄SlERFb2基因，并运用生物信息学对该基因的编码产物进行一级、二级、三级结构分析，并通过 qRT-PCR 技术检测SlERFb2基因在不同处理不同时期的表达量,以期为番茄对非生物胁迫的响应机制提供参考依据。结果表明：SlERFb2基因含有开放阅读框5个，共编码258个氨基酸，属于酸性蛋白；其二级结构以α-螺旋为主，预测蛋白定位于细胞核上；且含有39个磷酸化位点与15个糖基化位点；启动子区主要以I-box为主，且含有多个诱导基因表达的顺势作用元件；三级结构相对简单，且该转录因子在番茄中存在4个互作蛋白，系统发育树得番茄与马铃薯的亲缘关系最近；qRT-PCR显示该基因对低温的响应特别敏感。
- Abstract:
- The SlERFb2 gene of tomato was cloned,and the structure of the encoded protein was analyzed by using bioinformatics methods,and the expression level of the SlERFb2 was detected by qRT-PCR in different treatments and different periods, in order to provide some theoretical basis for the response mechanism of tomato to abiotic stress.The results showed that the SlERFb2 contained 5 openreading frames,encoding a total of 258 amino acids,which was an acidic protein;its secondary structure was mainly α-helix,and the predicted protein was located on the nucleus;and contained 39 phosphorylationsites with 15 glycosylation sites;the promoter region was mainly I-box,and contained multiple homeopathic elements that induce gene expression;the tertiary structure was relatively simple,and the transcription factor had 4 interactions in tomato protein,phylogenetic tree,the closest relationship between tomato and potato;qRT-PCR showed that the gene was particularly sensitive to low temperature response.
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备注/Memo
第一作者简介：金宝霞（1995-），女，硕士研究生，研究方向为樱桃番茄抗黄化曲叶病毒病。E-mail:3506451627@qq.com.责任作者：魏小红（1964-），女，博士，教授，博士生导师，现主要从事植物逆境生理与分子生理等研究工作。E-mail:weixh@gsau.edu.cn.基金项目：国家自然科学基金资助项目（32060401）；甘肃省教育厅优秀研究生“创新之星”资助项目（2021CXZX-001）。收稿日期：2021-06-08