LI Peng,XIANG Zhun,XIONG Xue,et al.Effects of Different Cultivated Substrates as Inducers on Laccase Activities of Auricularia cornea in Submerged Fermentation[J].Northern Horticulture,2021,(24):129-135.[doi:10.11937/bfyy.20211756]
不同栽培基质诱导对毛木耳产漆酶活性的影响
- Title:
- Effects of Different Cultivated Substrates as Inducers on Laccase Activities of Auricularia cornea in Submerged Fermentation
- 文献标志码:
- A
- 摘要:
- 以3个毛木耳菌株为试材，采用双因素方差分析，研究了木屑、玉米芯、棉籽壳和麦麸4种栽培基质诱导毛木耳菌株产漆酶活性的影响，以期为提高和改善毛木耳的产量和品质提供参考依据。结果表明：不同菌株产漆酶活性极显著不同（P<0.001），培养基质对漆酶活性也产生极显著的影响（P<0.001）。供试菌株分泌的漆酶活性依次为毛木耳781＞玉木耳01＞漳耳43-28，且毛木耳781和玉木耳01分泌的漆酶活性最大,分别为355.28、334.44 U·L-1，而漳耳43-28仅为92.11 U·L-1。此外，在含麦麸的基础培养基中3个毛木耳菌株漆酶活性普遍高于含棉籽壳、木屑或玉米芯的基础培养基，说明麦麸对不同毛木耳菌株产漆酶的诱导作用更强。该研究得到了毛木耳菌株高产漆酶的栽培基质。
- Abstract:
- Three kind of Auricularia cornea strains were used as test materials,and the two-factor analysis of variance was used to study the effects of wood chips,corn cobs,cotton seed husks and wheat bran on the laccase production of Auricularia cornea strains,in order to lay a theoretical foundation for increasing and improving the yield and quality of Auricularia cornea.The results showed that the laccase activity of different Auricularia cornea strains was extremely different (P<0.001),and the culture medium also had a significant impact on the laccase activity (P<0.001).The overall order of the laccase activity secreted by the tested strains was Auricularia cornea 781>new white Auricularia cornea 01>Zhang′er 43-28.The maximum activity of laccase secreted by Auricularia cornea 781 and new white Auricularia cornea 01 were 355.28 U·L-1,334.44 U·L-1,and the maximum activity of laccase secreted Zhang′er 43-28 was only 92.11 U·L-1.In addition,the laccase activity of the Auricularia corneastrains in the basic medium containing wheat bran was generally higher than that of the basic medium containing cotton seed hulls,wood chips or corn cobs,indicating that the wheat bran had a better induction on laccase production of different Auricularia corneastrains.
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备注/Memo
第一作者简介：李鹏（1987-），男，硕士，助理研究员，现主要从事食用菌资源应用与开发等研究工作。E-mail:761410952@qq.com.责任作者：向准（1976-），男，苗族，硕士，副研究员，现主要从事食用菌品种选育与栽培等研究工作。E-mail:564375391@qq.com.基金项目：贵州省科技计划资助项目(黔科合支撑[2017]2516号，黔科合支撑[2019]2332号)；黔科合重大专项资助项目([2019]3007号，[2019]3004-4号，ZWCQ[2019]3013-5号)；贵州省科技平台及人才团队计划资助项目(黔科合平台人才[2017]5720号)。收稿日期：2021-04-22