YANG Jiale,FAN Guizhi,ZHANG Lixiang.Effects of Colchicine Mutagenesis on Growth and Active Ingredients in Hyphae of Phellinus igniarius[J].Northern Horticulture,2021,(07):110-116.[doi:10.11937/bfyy.20204446]
秋水仙素诱变对桑黄菌丝生长及活性成分的影响
- Title:
- Effects of Colchicine Mutagenesis on Growth and Active Ingredients in Hyphae of Phellinus igniarius
- Keywords:
- Phellinus igniarius; protoplast; colchicine; active ingredients
- 文献标志码:
- A
- 摘要:
- 以1.0%纤维素酶和0.5%蜗牛酶不同处理时间的桑黄菌丝为试材，采用0.1%秋水仙素诱变桑黄菌丝研究其诱变下桑黄菌丝再生菌株的生长速率以及多糖、黄酮和三萜等活性成分含量变化，以期获得生长快且活性成分含量高的菌种，以期为桑黄菌株选育、种质资源创新和应用奠定基础。结果表明：秋水仙素诱变后桑黄菌丝再生菌株的生长速率以及多糖、黄酮和三萜等活性成分含量基本呈增加趋势。其中，与对照相比，诱变酶解桑黄菌丝20 min获得的再生菌株20-1生长速率最快，增加了366.67%；三萜和多糖含量最高的再生菌株为10-5，分别增加了1 069.73%和281.85%；多酚含量最高的再生菌株为5-1，增加了530.56%；黄酮含量最高的再生菌株为30-1，增加了3 771.43%。
- Abstract:
- Phellinus igniarius hyphae treated with 1% cellulase and 0.5% snail enzyme for different time were used as materials.Growth rate and changes of active ingredients such as polysaccharides,flavonoids and triterpenes of Phellinus igniarius mycelial regeneration strains inducedby 0.1% colchicine were analyzed，in order to obtain strains with fast growth and high content of active ingredients and lay the foundation for the selection and breeding of Phellinus igniarius strains,innovation and application of germplasm resources.The results showed that the growth rate and the content of active ingredients such as polysaccharides,flavonoids and triterpenes were basically increased after colchicine treatment.Among them,compared with the control,the regenerated strain 20-1 obtained by mutagenizing the hyphae of Phellinus igniarius for 20 minutes had the fastest growth rate,which increased by 366.67%.The regenerated strains with the highest content of triterpenes and polysaccharides were 10-5,which increased by 1 069.73% and 281.85%,respectively.The regenerated strain with the highest polyphenol content was 5-1,which increased by 530.56%.The regenerated strain with the highest flavonoid content was 30-1,which increased by 3 771.43%.
参考文献/References:
[1]徐鑫,边勇,陈哲,等.基于DNA条形码的桑黄真菌分子鉴定[J].北京农学院学报,2019,34(1):24-31.[2]韩东岐,吴海涛,王铁杰,等.桑黄纤孔菌发酵液化学成分的研究[J].中成药,2018,40(1):126-129.[3]陈建华,孙捷,吕福娇,等.桑黄发酵液中凝集素SHL24的分离与生物活性研究[J].微生物学通报,2018,45(2):420-427.[4]ZHU H,SUN S,ZHANG S.Enhanced production of total flavones and exopolysaccharides via Vitreoscilla hemoglobin biosynthesis in Phellinus igniarius[J].Bioresource Technology,2011,102(2):1747-1751.[5]孙婷婷.桑黄鲨烯环氧酶基因原核表达及过表达载体的构建[J].中草药,2018(11):2632-2639.[6]何培新,吴双双,郑凯,等.杨树桑黄胞外多糖的分子结构及抗氧化活性[J].食品与生物技术学报,2018，37(9):49-57.[7]LUNG M Y,TSAI J C,HUANG P C.Antioxidant properties of edible basidiomycete Phellinus igniarius in submerged cultures[J].Journal of Food Science,2010,75(1):18-24.[8]ZHENG S,DENG S,HUANG Y,et al.Anti-diabetic activity of a polyphenol-rich extract from Phellinus igniarius in KK-Ay mice with spontaneous type 2 diabetes mellitus[J].Food & Function,2017,9(1):614-623.[9]邵伟,乐超银,李玲,等.富硒香菇菌种原生质体诱变选育[J].食用菌,2004,26(2):12-13.[10]林英,吕淑霞,张蓓蓓,等.绿色木霉原生质体诱变筛选纤维素酶高产菌株[J].生物技术,2006,16(2):50-51.[11]KHATTAB A A,BAZARAA W A.Screening，mutagenesis and protoplast fusion of Aspergillus niger for the enhancement of extracellular glucose oxidase production[J].Journal of Industrial Microbiology & Biotechnology,2005,32(7):289-94.[12]竹文坤,贺新生.红平菇化学诱变育种研究[J].食用菌,2008,30(4):17-19.[13]金凌云.蛹虫草原生质体诱变及主要活性物质提取工艺研究[D].杨凌:西北农林科技大学,2010.[14]白英豪,张晓丽,李明军.秋水仙素诱导丹参多倍体研究进展[J].江苏农业科学,2018,46(5):18-20.[15]陈于和,秦素平,张瑞圃.秋水仙素处理对黑麦根尖细胞的诱变效应[J].河北科技师范学院学报,2006,20(1):10-12.[16]刘璐璐,柴明良.秋水仙素在果树多倍体育种中的研究进展[J].北方园艺,2006(6):48-49.[17]许兴泽,赵桂琴,柴继宽,等.秋水仙素对二倍体燕麦的诱变效果[J].草业科学,2018,35(11):97-106.[18]乔永刚,马璐琳,赵晓明,等.秋水仙素诱导党参四倍体[J].核农学报,2009,23(4):566-571.[19]徐年生,罗信昌,艾德甫.香菇新菌株选育[J].菌物研究,2012,10(4):255-262.[20]黄丽洋,石卉,王雅婷,等.野生桑黄菌株的分离鉴定和次生代谢物分析[J].中草药,2013,44(23):3394-3399.[21]张志胜,谢利,萧爱兴,等.秋水仙素处理兰花原球茎对其生长和诱变效应的影响[J].核农学报,2005(1):19-23.[22]吴青青,胡小京,崔嵬,等.秋水仙素诱导百合黄精灵多倍体研究[J].种子,2019,38(11):96-100.[23]陈敏敏,周音,孙亿敬,等.秋水仙素诱导百合多倍体及流式细胞仪倍性鉴定研究[J].上海农业学报,2018,34(2):81-87.[24]周霞,张尧,张成霞,等.原生质体诱变选育高产多糖荷叶离褶伞菌株[J].江苏农业科学,2017,45(16):126-128.[25]汤佳鹏,汪建雄.紫外-硫酸二乙酯复合诱变高通量筛选虫草素高产蛹虫草突变株[J].食品工业科技,2018,39(3):97-100.[26]董玉玮,苗敬芝,曹泽虹,等.灵芝原生质体化学诱变育种[J].食品研究与开发,2012,33(6):174-178.[27]陈颖,贾艳菊,张翠茹.拟南芥叶肉细胞原生质体分离及影响因素[J].河北大学学报(自然科学版),2008(4):423-426.[28]江力,孔小卫,吴晓杰,等.烟草原生质体的分离纯化[J].安徽大学学报(自然科学版),2006(6):91-94.[29]孔瑶,冯娇娇,张政权,等.玉米根尖组织原生质体的分离和流式分析[J].农业生物技术学报,2018,26(12):2160-2167.[30]席海秀,艾可筠,佟少明.普通小麦幼苗叶肉细胞原生质体分离方法的优化[J].生物技术通报,2016,32(4):68-73.[31]徐国前,张振文,郭安鹊,等.植物多酚抗逆生态作用研究进展[J].西北植物学报,2011,31(2):423-430.[32]刘松.极端干旱环境下植物体内多酚类物质含量及其对逆境的响应研究[D].北京:北京林业大学,2007.
相似文献/References:
[1]张金鹏,杨 晶,韩玉珠,等.植物体细胞杂交的研究进展[J].北方园艺,2014,38(01):192.
ZHANG Jin-peng,YANG Jing,HAN Yu-zhu,et al.Research Progress of Plant Somatic Hybridization[J].Northern Horticulture,2014,38(07):192.
[2]邓红梅,马超,王颖.黄瓜幼苗子叶原生质体的分离纯化及融合条件优化研究[J].北方园艺,2013,37(11):6.
DENG Hong-mei,MA Chao,WANG Ying.Study on the Optimal of Separation,Purification and Fusion Conditions of Cucumber Seedlings Cotyledons Protoplast[J].Northern Horticulture,2013,37(07):6.
[3]徐琴,王晓军,王卉,等.苘麻叶肉原生质体的分离和培养研究[J].北方园艺,2013,37(07):170.
XU Qin,WANG Xiao-jun,WANG Hui,et al.Study on Isolation and Culture of the Mesophyll Protoplasts from Abutilon avicennae[J].Northern Horticulture,2013,37(07):170.
[4]王昱,王义,王康宇,等.灵芝原生质体的制备与再生研究[J].北方园艺,2013,37(16):184.
WANGYu,WANGYi,WANGKang-yu,et al.StudyonPreparationandRegenerationoftheProtoplastsfrom Ganodermalucidum[J].Northern Horticulture,2013,37(07):184.
[5]王谦,马旭光,刘敏,等.原生质体紫外诱变选育猴头菌株[J].北方园艺,2014,38(16):145.
WANG Qian,MA Xu-guang,LIU Min,et al.Selection for a Hericium erinaceus Mutant Strain in the Way of Protoplasts Being Exposed to the UV Radiation[J].Northern Horticulture,2014,38(07):145.
[6]王卉,宁慧霞,刘敏,等.莴苣原生质体的分离方法[J].北方园艺,2012,36(11):109.
WANGHui,NINGHui-xia,LIUMin,et al.StudyontheMethodofIsolationProtoplastsofLactucasativaL.[J].Northern Horticulture,2012,36(07):109.
[7]肖 望,涂红艳,邓崇会.红姜花胚性细胞悬浮体系的建立和原生质体培养[J].北方园艺,2015,39(05):104.[doi:10.11937/bfyy.201505032]
XIAO Wang,TU Hong-yan,DENG Chong-hui.Establishment of Embryogenic Cell Suspension and Protoplast Culture of Hedychium coccineum[J].Northern Horticulture,2015,39(07):104.[doi:10.11937/bfyy.201505032]
[8]姜 明,刘岩,王 伟 功,等.不同pH 值和培养基对桑黄菌丝生长的影响[J].北方园艺,2010,34(08):0.[doi:10.11937/bfyy.201008074]
JIANG Ming,LIU Yan,WANG Wei O gong,et al.Effect of Different pH Values and Culture Medium on Mycelial Growth of Phellinus[J].Northern Horticulture,2010,34(07):0.[doi:10.11937/bfyy.201008074]
[9]温 海 祥,肖 洪 东,黄 剑 波,等.草菇种内原生质体融合育种研究[J].北方园艺,2000,24(03):0.[doi:10.11937/bfyy.200003037]
[J].Northern Horticulture,2000,24(07):0.[doi:10.11937/bfyy.200003037]
[10]龚光禄,桂阳,雷震,等.药用真菌桑黄（I.sanghuang）母种培养基的筛选[J].北方园艺,2016,40(24):139.[doi:10.11937/bfyy.201624035]
GONG Guanglu,GUI Yang,LEI Zhen,et al.Selection of Mother Culture Media of Medicinal Fungus Inonotus sanghuang[J].Northern Horticulture,2016,40(07):139.[doi:10.11937/bfyy.201624035]
备注/Memo
第一作者简介：杨佳乐（1995-），男，硕士研究生，研究方向为植物资源利用。E-mail:269827921@qq.com.责任作者：张李香（1977-），女，博士，副教授，现主要从事植物资源利用等研究工作。E-mail:fjszhanglx@126.com.基金项目：哈尔滨市科技局资助项目（2014RFQXJ066）；黑龙江省高校基本科研业务费黑龙江大学专项资金资助项目（HDJCCX-201617）。收稿日期：2020-10-23