LU Min,ZHANG Xiaoying,HUANG Yaxin,et al.LU Min1,ZHANG Xiaoying1,HUANG Yaxin2,WU Sufang2,AN Huaming1[J].Northern Horticulture,2020,44(24):16-25.[doi:10.11937/bfyy.20200756]
‘贵长’猕猴桃幼果中脱落酸的分布及相关基因的表达
- Title:
- LU Min1,ZHANG Xiaoying1,HUANG Yaxin2,WU Sufang2,AN Huaming1
- Keywords:
- Actinidia deliciosa; abnormal fruit; abscisic acid; gene expression
- 文献标志码:
- A
- 摘要:
- 以‘贵长’猕猴桃幼果为试材，研究了正常果与畸形果中脱落酸的分布及相关基因的表达，以期探究‘贵长’猕猴桃畸形果发生的可能原因。结果表明：‘贵长’猕猴桃畸形果和正常果中脱落酸（ABA）总含量相当，差异在于其分布的不同；相对于正常果而言，畸形果中部的ABA含量显著升高，而两侧的ABA含量显著下降。对4个ABA合成相关基因进行qPCR分析，其中[STBX]ZEP1[STBZ]在正常果两侧表达量最高，[STBX]NCED2在正常果中部和畸形果两侧表达量高，NCED3在畸形果两侧表达量最高，ABA2[STBZ]则在正常果中部表达量最高。对4个ABA分解及信号转导相关基因进行qRT-PCR分析，其中[STBX]CYP707A2、PP2C1和PP2C6均在正常果中部表达量最高；而CYP707A2l[STBZ]则在正常果两侧表达量最高。相对于正常果而言，在4个ABA合成相关基因中，[STBX]ABA2和NCED2[STBZ]在畸形果中部基因表达显著下降，而在两侧显著上升；在4个ABA分解及信号转导相关基因中，[STBX]CYP707A2和PP2C1[STBZ]在畸形果中部和两侧表达量均显著下降。该研究结果可为揭示猕猴桃畸形果的形成提供参考依据。
- Abstract:
- In order to study the possible causes of malformation of kiwi fruit,young fruits of ‘Guichang’ were used as materials.The distribution of abscisic acid in normal fruits and abnormal fruits and the expression of related genes were analyzed.The results indicated that the total ABA content in the abnormal and normal kiwi fruits was similar,but the difference was the distribution.Compared with normal fruits,the ABA content in the middle of abnormal fruits increased significantly,while that in flanking sides decreased significantly.Four genes related to ABA synthesis were analyzed by qRT-PCR,in which the expression level of [STBX]ZEP1[STBZ] was the highest on flanking sides of normal fruit,[STBX]NCED2 was the highest in the middle of normal fruits and on flanking sides of abnormal fruits,NCED3 was the highest on flanking sides of abnormal fruits,and ABA2 was the highest in the middle of normal fruits.Four genes related to ABA catabolism and signal transduction were also analyzed by qRT-PCR.The expression levels of CYP707A2,PP2C1 and PP2C6 were the highest in the middle of normal fruits.However,the expression of CYP707A2l was the highest on flanking sides of the normal fruit.Compared with normal fruit,of the four genes related to ABA synthesis,ABA2 and NCED2 were significantly decreased in the middle of abnormal fruits and significantly increased on flanking sides.Among the four genes related to ABA catabolism and signal transduction,the expression levels of CYP707A2 and PP2C1[STBZ] were significantly decreased in the middle and flanking sides of abnormal fruits.The results provided theoretical basis for revealing the formation of abnormal kiwi fruits.
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备注/Memo
第一作者简介：鲁敏(1984-)，女，博士，副教授，现主要从事果树种质资源与遗传育种等研究工作。E-mail：48181266@qq.com.责任作者：安华明(1973-)，男，博士，教授，现主要从事果树生理与分子生物学等研究工作。E-mail：anhuaming@hotmail.com.基金项目：贵州省修文县猕猴桃产业发展资助项目（H180034）。收稿日期：2020-03-03