[1]范琳,朱晶桓,苏跃,等.黑龙江省马铃薯黑痣病菌遗传多样性分析[J].北方园艺,2020,44(14):42-49.[doi:10.11937/bfyy.20194134]
　FAN Lin,ZHU Jinghuan,SU Yue,et al.Genetic Diversity of Rhizoctonia solani From Potato in Heilongjiang Province[J].Northern Horticulture,2020,44(14):42-49.[doi:10.11937/bfyy.20194134]
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黑龙江省马铃薯黑痣病菌遗传多样性分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年14 页码: 42-49 栏目: 出版日期: 2020-07-30
Title:
Genetic Diversity of Rhizoctonia solani From Potato in Heilongjiang Province
作者:
范琳1; 朱晶桓2; 苏跃3; 杨明秀1; 宋爽1; 张俊华1
（1.东北农业大学 农学院，黑龙江 哈尔滨　150030；2.甘南县扶贫开发服务中心,黑龙江 齐齐哈尔 162100；3.山东科塞基农控股有限公司，山东 济南 250101）
Author(s):
FAN Lin1; ZHU Jinghuan2; SU Yue3; YANG Mingxiu1; SONG Shuang1; ZHANG Junhua1
(1.School of Agriculture，Northeast Agricultural University，Harbin，Heilongjiang 150030;2.Poverty Alleviation and Development Service Center of Gannan County,Qiqihaer，Heilongjiang 162100;3.Shandong Kesai Agrochem Holdings Co.Ltd.,Jinan,Shandong 250101)
关键词:
马铃薯; 遗传多样性; SSR
Keywords:
potato; genetic diversity; SSR
DOI:
10.11937/bfyy.20194134
文献标志码:
A
摘要:
以黑龙江省7个马铃薯种植区采集并分离得到的204个马铃薯黑痣病菌菌株为试材，采用SSR分子标记技术方法，研究了菌株遗传差异性，分析了黑龙江省马铃薯黑痣病菌遗传多样性。结果表明：使用选取的10对SSR引物对204个供试菌株进行扩增，其中10对引物均可对供试菌株扩增出多态性好、清晰和稳定的条带。通过聚类分析可知，遗传距离为2.577 09时划分为4个类群，各类群菌株分布复杂，表明黑龙江省马铃薯黑痣病病菌遗传类型复杂多样，菌株的遗传多样性与样品采集地无明显相关性。
Abstract:
204 Rhizoctonia solani Kuhn strains collected and isolated from seven potato growing areas in Heilongjiang Province were used as test materials，and SSR molecular marker technology was used to study the genetic differences of the strains in order to study the genetic diversity of Rhizoctonia solani Kuhn strains.The results of genetic diversity analysis showed that 10 pairs of SSR primers were used to amplify 204 tested strains，in which 10 pairs of primers could amplify polymorphic，clear and stable bands for the tested strains.According to the cluster analysis，it was found that 204 strains were divided into 4 groups when the genetic distance was 2.577 09 and the distribution of ever groups was complicated.The results showed that the genetic diversity of Rhizoctonia solani Kuhn from potato in Heilongjiang Province was complex and varied and the genetic diversity of the strain was also not significantly correlated with the sample collection.

参考文献/References:

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备注/Memo

第一作者简介：范琳（1994-），女，硕士研究生，研究方向为植物病理学。E-mail：872547808@qq.com.责任作者：张俊华（1973-），男，博士，教授，博士生导师，现主要从事植物病理学等研究工作。E-mail：1132919642@qq.com.基金项目：黑龙江省自然科学基金资助项目（c2017032）；中央引导地方科技发展专项资助项目（ZY16C06）。收稿日期：2019-04-05

更新日期/Last Update: 2020-09-15