[1]钟灿,劳嘉,金剑,等.基于激素及其配比探讨多花黄精种子组培体系的建立[J].北方园艺,2020,44(13):118-123.[doi:10.11937/bfyy.20194082]
　ZHONG Can,LAO Jia,JIN Jian,et al.Effects of Hormones and Their Ratios on the Establishment of Tissue Culture System for the Seeds of Polygonatum cyrtonema Hua[J].Northern Horticulture,2020,44(13):118-123.[doi:10.11937/bfyy.20194082]
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基于激素及其配比探讨多花黄精种子组培体系的建立

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年13 页码: 118-123 栏目: 出版日期: 2020-07-15
Title:
Effects of Hormones and Their Ratios on the Establishment of Tissue Culture System for the Seeds of Polygonatum cyrtonema Hua
作者:
钟灿1; 劳嘉2; 金剑1; 贺炜2; 王维子1; 张水寒1
（1.湖南省中医药研究院 中药研究所，湖南 长沙 410013；2.绿之韵生物工程集团有限公司，湖南 长沙 410329）
Author(s):
ZHONG Can1; LAO Jia2; JIN Jian1; HE Wei2; WANG Weizi1; ZHANG Shuihan1
(1.Institute of Chinese Materia Medica,Hunan Academy of Chinese Medicine,Changsha,Hunan 410013;2.Resgreen Group International Inc.,Changsha,Hunan 410329)
关键词:
多花黄精; 细胞分裂素; 生长素; 种子; 消毒
Keywords:
Polygonatum cyrtonema Hua; cytokinin; auxin; seeds; sterilization
DOI:
10.11937/bfyy.20194082
文献标志码:
A
摘要:
以湖南大宗药材多花黄精种子为试材，研究不同消毒方法、单一激素对种子污染率和萌发率的影响，不同激素配比对种子芽生长和根系的诱导，以期建立多花黄精种子组培体系。结果表明：用8%NaClO消毒10~15 min配合0.1%HgCl2消毒10~15 min，污染率低，种子萌发率在55%~65%；温度为（26±2）℃，光照强度为2 000 lx条件下，每天光照12 h，当年采收的种子萌发率显著高于贮藏1年的种子，单一激素下种子的萌发率较低，且不同激素对种子萌发的诱导存在差异。激素配比能显著提高种子的萌发率，1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA培养基组合有利于芽的生长；1.0 mg?L-1 IBA+0.5 mg?L-1 NAA培养基组合最有利于生根，培育周期8~10个月。该研究为多花黄精的育苗和品种改良提供一定技术支持。
Abstract:
The seeds of Polygonatum cyrtonema Hua were used as test materials to establish a high-efficient in vitro regeneration system.Effect of sterilization,single hormone on seed germination,and hormone ratio on bud growth and root induction were studied .The results showed that the optimal sterilization was using 8% NaClO for 10-15 minutes and 0.1% HgCl2 for 10-15 minutes was good forreducing pollution rate and germination rate between 55%-65%.The germination rate of seeds harvested in the same year was significantly higher than that of seeds stored for one year.Single hormone was not good for seeds gemination.The combination of 1.0 mg?L-1 6-BA+0.5 mg?L-1 NAA benefited to the elongation of adventitious buds.And 1.0 mg?L-1 IBA+0.5 mg?L-1 NAA was the best combination of hormones for rooting,and the temperature was (26±2)℃ with 2 000 lx light intensity for 12 hours one day.The cultivation period was 8-10 months.This study provided a technical support for seedling and variety improvement of removal of Polygonatum cyrtonema Hua.

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备注/Memo

第一作者简介：钟灿（1986-），女，博士，助理研究员，研究方向为中药资源。E-mail:canzhong651@163.com.责任作者：张水寒（1966-），女，博士，研究员，博士生导师，研究方向为中药资源。E-mail:zhangshuihan0220@126.com.基金项目：特色中药资源综合开发与利用资助项目（2060302）；湖南省中医药研究院自主科研计划资助项目（201705）；2018年中医药公共卫生服务补助专项“全国中药资源普查”资助项目（财社[2018]43号）；2015年中医药部门公共卫生服务补助资金资助项目（财社[2015]78号）；2019年医疗服务与保障能力提升补助资金资助项目（财社[2019]39号）。收稿日期：2020-01-13

更新日期/Last Update: 2020-09-09